CBQF - Documentos de Conferências / Conference Objects
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- 2-Fluorophenol degradation by aerobic granules in a sequencing batch reactorPublication . Duque, A. F.; Bessa, V. S.; Carvalho, M. F.; De Kreuk, M. K.; Loosdrecht, M. C. M. van; Castro, P. M. L.
- 3D printed bioactive interference screw and PCL bio-filler for ligament fixationPublication . Rodrigues, Mafalda; Moreira, Rui; Silva, Inês V.; Duarte, Marta M.; Ribeiro, Viviana P.; Oliveira, Ana L.; Costa, João B.Musculoskeletal injuries, widespread across all ages, genders and sociodemographic groups, are prevalent in the knee joint and require a range of treatments - from conservative methods to surgical interventions, such as meniscal resection, repair, reconstruction or tissue engineering (TE) approaches. To address one of the most significant challenges in orthopedic procedures – long-term implant fixation – an innovative solution is being developed for knee ligaments and meniscus fixation. PLA screws are being developed through the combination of 3D printing, supercritical CO2 (scCO2) foaming and impregnation technologies, aiming to achieve a biodegradable and bioactive screw with improve bone integration ability. Additionally, to improve the anchor and fixation of the ligament treatments, PCL Bio-Fillers are being developed through the use of 3D printing, electrospinning and dipping methods with the final goal to induce the bone cells to reproduce itself (osteogenesis) and create a better grip between the ligaments and the bone. PLA screws were manufactured by 3D printing and further process to induce porosity by scCO2 foaming, followed by EPS impregnation through scCO2. Scanning electron microscopy (SEM) was used to evaluate microporosity and the EPS impregnation. The CO2 concentration, density and expansion ratio of the PLA screws were evaluated. FTIR (Fourier-transform infrared spectroscopy) was performed to evaluate chemical composition changes of the samples. DSC (Differential scanning calorimetry) was applied to analyze thermal stability both before and after treatment. PCL Bio-Fillers were produced by 3D printing, coated with PCL using electrospinning and dipped with bruxite. Then, to study the PCL fibers and bruxite dispersion, we have used the scanning electron microscopy (SEM) method. Results: Several conditions of foaming were tested (pressure, time, temperature and controlled expansion measures) and then, analyzed through SEM imaging. Samples with greater porosity were selected for further testing and analysis. The CO2 concentration results revealed that the saturation increase is proportional to the increase in pressure and inversely proportional to the increase in infill density. The expansion ratio results demonstrated that it typically decreases with increasing infill density and batch pressure. To optimize the 3D printed Bio-Fillers coated with PCL several parameters were adjusted (PCL concentration, flow rate, distance, potential difference, and nozzle size). Firstly, samples with apparent macroscopy uneven coating were remove with further analysis being performed via SEM analysis. The SEM analysis showed that increasing the potential difference and decreasing the flow rate produced more dispersed and thinner fibers. It also revealed that increase PCL concentration led to higher fiber density and size. In the end, the parameters that resulted in the better PCL fibers dispersion were with a concentration of 7,5% w/v of PCL, 20 μL/min flow rate, 10 cm of distance between the nozzle and the Bio-Filler, 23 kV of potential difference and 20 G of nozzle size. Further work is being performed to optimize the dipping process with bruxite and achieve an even coating. Conclusion: The sCO2 methodologies implemented were efficient in terms of generating porosity and EPS impregnation. EPS-induced bioactivity will be studied in the future.
- 4-fluorocinnamic acid biodegradation by a rhodococcus strainPublication . Amorim, Catarina L.; Carvalho, M. F.; Afonso, C. M. M.; Castro, Paula M. L.
- Ability of leuconostoc lactis RK18 to prevent adhesion and invasion of human intestinal cells by foodborne pathogen listeria monocytogenesPublication . Oliveira, Mónica; Barbosa, Joana; Albano, Helena; Teixeira, Paula
- ABioNet: rede de ciência e tecnologia em bioprodutos na ArméniaPublication . Oliveira, Leandro; Santos, Ana Sofia; Cardoso, Eduardo Luís
- Ação antimicrobiana de extratos fenólicos de cogumelos silvestres contra Neisseria gonorrhoeaePublication . Alves, Maria José; Marques, Azucena; Ferreira, Isabel C. F. R.; Martins, Anabela; Pintado, ManuelaInfeções pelo gonococo e por clamídia estão entre as doenças sexualmente transmissíveis mais comuns causadas por bactérias no mundo. Por outro lado, tem-se verificado um aumento de resistência da Neisseria gonorrhoeae aos diferentes antimicrobianos usados no mercado, sendo desta forma urgente a descoberta de novas soluções antimicrobianas no combate a esta infeção. Assim, o objetivo do presente trabalho foi avaliar a atividade antimicrobiana de extratos e respetivos compostos fenólicos puros de nove espécies de cogumelos silvestres, provenientes do Nordeste de Portugal, contra Neisseria gonorrhoeae obtida de exsudados da uretra no CHTMAD – Centro Hospitalar de Trás-os-Montes e Alto Douro. Para a determinação das concentrações mínimas inibitórias (CMI) de crescimento recorreu-se ao método da microdiluição e ao ensaio colorimétrico com cloreto de p-iodonitrotetrazólio (INT). De um modo geral, todos os extratos apresentaram atividade antimicrobiana sobre a Neisseria gonorrhoeae, e alguns compostos parecem apresentar boa atividade antimicrobiana para esta bactéria, tornando-se pertinente estudar o mecanismo de ação destes compostos na inibição da bactéria bem como a sua toxicidade celular no sentido de avaliar possíveis aplicações clínicas na terapêutica e mesmo na prevenção da gonorreia.
- Acceptability of reengineered Hibiscus drinks by Senegalese consumersPublication . Monteiro, M. J.; Cisse, M.; Fliedel, G.; Bechoff, A.; Bouche, M.; Pallet, D.; Tomlins, K.; Ayessou, A.; Costa, A. I. A.; Pintado, M. M.Bissap is a non-alcoholic drink commonly consumed in African countries, particularly in Senegal. It is made from Hibiscus sabdariffa L. - an herbaceous plant belonging to the Malvaceae, most often from its Ordinary/Kor (Senegal) and/or Vimto (Sudan) varieties. Past research has shown that Hibiscus drinks are generally rich in vitamins, minerals and bioactive compounds. These drinks are amongst the products investigated by AFTER, an EU FP7-funded research project aiming at the production of good quality (nutritional and sanitary) and extended shelf-life products of African tradition for local and European markets. A previous AFTER study on the acceptability of four traditional Hibiscus drinks by a sample of Senegalese consumers uncovered significant effects of plant variety and processing method. This highlighted the importance of harmonizing the sensory profile of these drinks as part of the product re-engineering process, and re-assessing their acceptability amongst the Senegalese population. In view of this, three new Hibiscus (50% Kor and 50% Vinto) drinks – an infusion, a syrup and a vacuum-concentrate – were developed by AFTER researchers. Their sensory quality was evaluated, alongside that of a traditional infusion (baseline), by a sample of 156 Senegalese in Dakar in October-November of 2013. Consumer profiling techniques based on hedonic acceptance, Just-About-Right intensity evaluation of specific descriptors (JAR) and Check-All- That-Apply questions with sensory and emotional descriptors (CATA) were used to establish sensory profiles and preference maps. Descriptors and other relevant evaluative information were obtained through two exploratory focus groups.Results show that the new Hibiscus drinks all had better acceptability than the baseline. Moreover, three distinct types of Senegalese consumers were identified: baseline dislikers, who liked all drinks except traditional one; overall likers; new Infusion dislikers. Finally, multiple factor analysis of overall liking scores, JAR ratings and CATA answers yield highly convergent results for all the drinks evaluated.
- Acceptance of exotic beverages with health benefits in Europe: a crosscountry comparison of hibiscus productsPublication . Monteiro, M. J. P.; Costa, A. I. A.; Fliedel, G.; Cisse, M.; Tomlins, K.; Pintado, M. E.
- Achieving efficient viability of akkermansia muciniphila during aerobic storage and gastrointestinal passage through calcium-alginate encapsulationPublication . Machado, Daniela; Fonseca, Mariana; Vedor, Rita; Barbosa, Joana Cristina; Gomes, Ana Maria
- Acorn flours: a novel source of bioactives and nutritional benefitsPublication . Rodrigues, Cristina V.; Maia, Maria Luz; Babo, Pedro; Pintado, ManuelaIntroduction: In Portugal, acorns (Quercus spp.) are highly abundant, being produced around 401,585 tonnes annually. Yet, about 55% remains in fields, leading to the waste of this biomass and promoting potential environmental issues (e.g. soil degradation) (Castro et al., 2022, Zhou and Wang, 2020). This challenge is further exacerbated by the increasing global population, which exerts mounting pressure on our food systems. However, acorn by-products hold great promise as a source of bioactive ingredients, offering solutions to maximize food utilization, promote upcycling, and introduce innovative nutritional and health benefits (Mahmud et al., 2022) . This potential is especially evident in acorns’ kernel, which is rich in valuable bioactive compounds suitable for the development of value-added products. The acorn kernel is highly abundant in phenolic compounds (e.g. tannins, quercetin, gallic acid), oil rich in fatty acids (e.g. oleic acid, linoleic acid), tocopherols, carotenoids (e.g. β-carotene), and phytosterols (e.g. β-sitosterol), which hold potential antioxidant, anti-inflammatory and anti-microbial effects (Szabłowska and Tańska, 2024). Moreover, starch recovered from the acorns’ kernel can be applied as a thickening and stabilizer agent in food formulations (Castro et al., 2024), and can hold promising characteristics, which can lead to the improvement of the gut microbiome. Objectives: The present work aimed to characterize the kernel flour from two acorn species: Quercus pyrenaica and Quercus rotundifolia, provided by LandraTech, in a way to valorize them, through potential food applications, promoting circular economy within the industry. Results and main conclusions: Overall, the valorization of the acorn raw materials, exemplified by the flours obtained from its kernel, not only offers promising ways for sustainable and novel food applications, but also underlines the importance of turning waste into valuable resources in the global food industry, as a circular economy approach.