CBQF - Documentos de Conferências / Conference Objects
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- Morphology-dependent antimicrobial and migration properties of ZnO nanoparticles in bio-based packaging applied to melon fruitPublication . Mendes, Ana Rita; Silva, Francisco A. G. Soares; Mena, Cristina; Silva, M. Fátima; Silva, Cristina L. M.; Teixeira, Paula; Poças, Fátima
- PARAFISH: a cross-platform graphical interface for reproducible metabarcoding analysis in aquatic parasite monitoringPublication . Faria, João; Rito, Teresa; Carneiro, João
- Integrating 3D structural modelling and probe design for enhanced environmental pathogen detectionPublication . Pereira, Beatriz; Fernandes, Romeu; Soares, Pedro; Pratas, Diogo; Sousa, Sérgio F.; Carneiro, João
- ViruScope: an integrated pipeline for literature-driven and in silico viral primer designPublication . Lima, Ana; Sousa, Sérgio F.; Sá, Vítor; Pratas, Diogo; Carneiro, João
- Monitoring stress levels with salivary alpha-amylase determination through a paper-based microfluidic analytical devicePublication . Melo, Maria M. P.; Rangel, António O. S. S.; Mesquita, Raquel B. R.
- Metabolite profiling of post-biotics obtained from fish by-productsPublication . Silva, Sara; Costa, Eduardo M.; Machado, ManuelaThe FERDINAND project aims to valorize fish by-products as a sustainable source of bioactive compounds for applications in cardiometabolic health, focusing on the development and characterization of innovative post-biotic fractions. Post-biotic metabolite analysis provides critical insight into the biochemical transformations occurring during microbial fermentation and supports the valorization of fish by-products into high-value functional ingredients. In this study, fish-derived biomass is fermented using selected microbial strains, generating a complex mixture of post-biotics formed during the breakdown of proteins, lipids, and residual organic matter. A comprehensive analytical framework combining chromatographic and mass spectrometry-based techniques is applied to characterize the resulting metabolites, including short-chain fatty acids, organic acids, and peptide fragments. Targeted quantification is performed using GC-FID and HPLC-RI, while LC-MS/MS and untargeted metabolomics enable in-depth profiling of low-molecular-weight compounds. Integrating targeted and untargeted data provides a holistic understanding of post-biotic composition, facilitating the identification of safe, stable, and bioactive fractions derived from fish by-products with potential applications in food, nutraceutical, and biomedical sectors.
- Bioactive phenolics from aromatic plants: chemical signatures and anti-inflammatory effects on intestinal cellsPublication . Machado, Manuela; Silva, Sara; Costa, Eduardo M.Aromatic plants constitute a rich and largely underexplored reservoir of bioactive phenolic compounds — flavonoids, hydroxycinnamic acids, and polyphenolic derivatives — whose structural diversity underlies a broad spectrum of bbiological activities. Their distinct chemical signatures, shaped by hydroxylation patterns, glycosylation states, and conjugation with organic acids, are key determinants of their bioavailability and therapeutic potency. These secondary metabolites have been shown to modulate critical inflammatory pathways in intestinal epithelial and immune cells, notably through inhibition of NF-?B signaling, downregulation of pro-inflammatory cytokines (IL-6, IL- 1?, TNF-?), and suppression of COX-2 and iNOS activity. Beyond direct anti-inflammatory action, phenolic compounds can reinforce epithelial barrier integrity and positively modulate gut microbiota composition, adding further relevance to their role in intestinal health. Inflammatory bowel conditions, characterized by chronic mucosal inflammation and significant impact on quality of life, remain a major therapeutic challenge — making the search for effective and safe natural alternatives increasingly urgent.
- Comparative proximate and phenolic characterization of winemaking sedimentsPublication . Costa, Eduardo M.; Vieira, Sofia; Machado, Manuela; Castro, Luís M.; Barros, Ana N.; Silva, SaraThe wine industry generates a wide range of solid residues throughout vinification, many of which remain underexploited despite their potential as sources of bioactive compounds. While byproducts such as pomace and conventional lees have been widely studied, less attention has been given to specific sediment fractions collected at defined stages of the process.In particular, sediments recovered after fermentation and prior to bottling represent a distinct and poorly characterised matrix. Unlike primary lees or pomace, these materials result from prolonged settling and transformation processes, potentially leading to unique compositional profiles shaped by fermentation dynamics, microbial activity, and chemical ageing.These sediments are expected to concentrate structural nutrients (e.g. proteins, fibres, minerals) alongside a diverse range of polyphenols, including phenolic acids, flavonoids, and stilbenes, compounds associated with antioxidant and health-promoting properties. However, their nutritional composition and phenolic signature remain largely unexplored, limiting their integration into valorisation strategies.
- Core–shell hydrogel based on supercritical CO2-decellularized pancreatic ECM for functional ?-cell encapsulation and immune protectionPublication . Pazmino, Carlos; Sá, Simone; Amorim, Sara; Oliveira, Ana L.Type 1 Diabetes Mellitus (T1DM) affects over 9 million people worldwide and is characterized by autoimmune destruction of insulin-producing pancreatic ?-cells. While exogenous insulin remains the primary treatment, inaccurate dosing often leads to poor glycaemic control. Pancreatic islet transplantation offers a potential curative approach; however, long-term success is hindered by immune rejection and poor graft vascularization. To address these limitations, we developed a permeable, pro-angiogenic, immune-isolating core–shell hydrogel that replicates native pancreatic microenvironment. The construct, a decellularized porcine pancreatic extracellular matrix (dECM) core, repopulated with insulin- producing ?-cells, and an alginate shell. Supercritical carbon dioxide (scCO?) was employed for tissue decellularization due to its capacity to efficiently penetrate the tissue and remove cellular components while preserving ECM biochemical integrity. Decellularization efficacy was confirmed by DNA quantification (Grisp GRS kit), endotoxin analysis (Pierce chromogenic kit), and assessment of dECM components (GAGs and collagen-Blyscan kits). The resulting dECM was enzymatically digested with pepsin and photocrosslinked using ruthenium/sodium persulfate (Ru/SPS) under visible light to form a stable hydrogel. Rheological analysis was performed to assess mechanical properties. To enhance stability and immune protection, the dECM-Ru core was encapsulated within a CaCl?-crosslinked alginate shell, generating the optimized dECM-Ru/Alg core–shell construct. Encapsulated ?-cells displayed high viability (Alamar Blue), proliferation (BrdU incorporation), and insulin secretion (immunostaining), confirming functional maintenance within the hydrogel. The construct demonstrated tuneable mechanical properties, nutrient permeability, and potential for vascular integration. This dECM-Ru/Alg core–shell hydrogel provides a biomimetic, immune-isolating platform supporting ?-cell survival and function, offering a promising strategy for cell-based therapies in T1DM.
- Effectiveness of three disinfectants on the removal of Listeria monocytogenes biofilmsPublication . Carvalho, Marta; Ribeiro, Deise Helena Baggio; Teixeira, Paula