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- Development of a microfluidic paper-based sensor for aluminium assessment in water for domestic consumptionPublication . Aguiar, Juliana I. S.; Rangel, António O. S. S.; Mesquita, Raquel B. R.
- Empowering rural communities: a novel paper-based sensor for monitoring aluminium in water used for domestic consumptionPublication . Aguiar, Juliana I. S.; Rangel, António O. S. S.; Mesquita, Raquel B. R.Ensuring the safety of domestic water sources is critical for public health, particularly in rural areas where well water is commonly used for household consumption. Aluminium, a naturally occurring element, can leach into groundwater, and long-term exposure to high aluminium concentrations has been linked to neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases. So, the aim was to develop a microfluidic paper-based analytical device (μPAD) for the accurate quantification of aluminium levels in well water, providing an affordable, portable, and user-friendly tool for well owners to monitor their water source quality. The μPAD was designed to attain aluminium determination using the colourimetric reaction with chrome azurol S (CAS). The device comprises two layers of paper, where the first layer is impregnated with a solution with three reagents: CAS, ascorbic acid, and acetate buffer solution. The CAS reagent reacts with aluminium(III) to produce a distinct colour product; ascorbic acid is used to eliminate interference from iron(III), and the acetate buffer solution ensures the appropriate reaction pH. The second layer of filter paper is empty to ensure a vertical flow approach as well as being a sample/standard reservoir. Several physical and chemical parameters of the device were optimised to attain the highest sensitivity for aluminium determination. Among the performed studies were the number of layers in the μPAD, the type and porosity of filter paper used, sample and reagent volumes, and reagents concentrations. The aluminium determination with the developed μPAD was validated using certified water samples and comparing the results obtained for several well-water with both the μPAD and AAS (atomic absorption spectrophotometry). After demonstrating its accuracy and robustness, the developed μPAD complies with the World Health Organization guidelines (having below 0.20 mg/L of aluminium in drinking water), enabling aluminium determination in the range of 0.10 to 1.00 mg/L. This μPAD provides a practical solution for regular monitoring of water quality, empowering well owners to take proactive steps in managing their water resources and ensuring their family’s health and safety.
- Effect of a chitosan-enriched biofertilizer on induced resistance against the pinewood nematode, bursaphelenchus xylophilusPublication . Nunes, Marta Nunes da; Carvalho, Vinícius G. B.; Sarmento, Bruno; Stamford, Newton P.; Pintado, Manuela E.; Vasconcelos, Marta W.
- A bi-parametric paper-based sensor for Al(III) and Fe(III) monitoring in well watersPublication . Aguiar, Juliana I. S.; Rangel, António O. S. S.; Mesquita, Raquel B. R.
- Morphology-dependent antimicrobial and migration properties of ZnO nanoparticles in bio-based packaging applied to melon fruitPublication . Mendes, Ana Rita; Silva, Francisco A. G. Soares; Mena, Cristina; Silva, M. Fátima; Silva, Cristina L. M.; Teixeira, Paula; Poças, Fátima
- PARAFISH: a cross-platform graphical interface for reproducible metabarcoding analysis in aquatic parasite monitoringPublication . Faria, João; Rito, Teresa; Carneiro, João
- Integrating 3D structural modelling and probe design for enhanced environmental pathogen detectionPublication . Pereira, Beatriz; Fernandes, Romeu; Soares, Pedro; Pratas, Diogo; Sousa, Sérgio F.; Carneiro, João
- ViruScope: an integrated pipeline for literature-driven and in silico viral primer designPublication . Lima, Ana; Sousa, Sérgio F.; Sá, Vítor; Pratas, Diogo; Carneiro, João
- Monitoring stress levels with salivary alpha-amylase determination through a paper-based microfluidic analytical devicePublication . Melo, Maria M. P.; Rangel, António O. S. S.; Mesquita, Raquel B. R.
- Metabolite profiling of post-biotics obtained from fish by-productsPublication . Silva, Sara; Costa, Eduardo M.; Machado, ManuelaThe FERDINAND project aims to valorize fish by-products as a sustainable source of bioactive compounds for applications in cardiometabolic health, focusing on the development and characterization of innovative post-biotic fractions. Post-biotic metabolite analysis provides critical insight into the biochemical transformations occurring during microbial fermentation and supports the valorization of fish by-products into high-value functional ingredients. In this study, fish-derived biomass is fermented using selected microbial strains, generating a complex mixture of post-biotics formed during the breakdown of proteins, lipids, and residual organic matter. A comprehensive analytical framework combining chromatographic and mass spectrometry-based techniques is applied to characterize the resulting metabolites, including short-chain fatty acids, organic acids, and peptide fragments. Targeted quantification is performed using GC-FID and HPLC-RI, while LC-MS/MS and untargeted metabolomics enable in-depth profiling of low-molecular-weight compounds. Integrating targeted and untargeted data provides a holistic understanding of post-biotic composition, facilitating the identification of safe, stable, and bioactive fractions derived from fish by-products with potential applications in food, nutraceutical, and biomedical sectors.