Browsing by resource type "conference object"
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- 1,3-Propanediol continuous production by Clostridium butyricum VP1 1718: effect of dilution rate and substrate concentrationPublication . Gonzalez-Pajuelo, M.; Ribeiro-Cruz, A. P.; Sousa- Monagas, C. M.; Andrade, J. C.; Vasconcelos, I.
- #113 A estabilidade da cor dos bulk fill pode ser influenciada pelo sistema adesivo?Publication . Belchior, Ana Sofia Leitão; Almeida, Carlos Ferreira de; Rodrigues, Cláudia Alexandra da Silva; Correia, André; Noites, Rita
- 2-Fluorophenol degradation by aerobic granules in a sequencing batch reactorPublication . Duque, A. F.; Bessa, V. S.; Carvalho, M. F.; De Kreuk, M. K.; Loosdrecht, M. C. M. van; Castro, P. M. L.
- 2015: O Ano em que se dá um passo em frente como quem dá três para trásPublication . Azevedo, Joaquim
- A 3d cell culture model of the tuberculosis granuloma that can be applied for host genetic studies in the context of a multicellular immunologic response to infectionPublication . David, Susana; Mandal, Manoj; Anes, Elsa; Pires, DavidIntroduction: The granuloma is an inflammatory infiltrate of mononuclear cells. Some bacterial infections are characterized by the formation of granulomas as part of the immune response to contain the infection. Granuloma models have contributed valuable insights into the genetic basis of granuloma formation during infection. For example, IFNGR1 and IFNGR2 variants have been found to disrupt the immune response, resulting in impaired granuloma formation and increased susceptibility to diseases by Mycobacterium sp. More easily implemented comprehensive models would facilitate the study of the different immune mechanisms and help identify new diseaseassociated genes. Our objective is to generate an in vitro 3D cell culture model using human primary cells and microspheres to generate a stratified granuloma model for future use in genetic, immunological and drug discovery studies. Methods: A commercial system was used to encapsulate human peripheral blood mononuclear cells (PBMC) infected with GFP-expressing M. tuberculosis and maintained in culture for several weeks. The cellular constituents of these granulomas and their organization were characterized by fluorescence microscopy and flow cytometry as well as the viability of the cells and the extent of bacterial replication in factor of time. Results: The results demonstrate a ready recruitment of cells towards infected macrophages, leading to the formation of densely populated aggregates. These aggregates maintained cell viability for several weeks and displayed an enhanced control of bacterial replication compared to the more common monolayer infection models. Moreover, the capsules can be easily disrupted when required to isolate genetic material for further analysis. Conclusion: The proposed 3D model resembles some structural and cellular characteristics of the tuberculosis granuloma and maintains its stability beyond more common 2D models of infection. These preliminary results demonstrate that this model can be used to further explore the determinants of granuloma formation and host response to infection.
- 3D printing objects as installation art: standing humanity: 3D yan characterPublication . Estadieu, Gérald; Abreu, Filipa Martins de; Barbosa, Alvaro3D printing technologies have been known for several decades and have been already used in arts, most often as accessories rather than as core material of a tangible installation art. We describe "Standing Humanity: 3D Yan Character" a collaborative and participative installation art based on 3D printing objects and we are analyzing participants' engagement and the playability of the installation. We analyze different types of interaction and conclude that 3D printing objects have a high potential to engage curiosity and open to interactions between visitors and the objects themselves. We finally suggest further investigations to explore 3D printing within the context of collaborative and interactive installation.
- 4-fluorocinnamic acid biodegradation by a rhodococcus strainPublication . Amorim, Catarina L.; Carvalho, M. F.; Afonso, C. M. M.; Castro, Paula M. L.
- À conversa sobre saúde mental, pobreza e exclusão socialPublication . Palha, Filipa
- À quarta…família com arte: como potenciar o envolvimento parental no ensino artístico especializado da músicaPublication . Orvalho, Luísa; Duarte, HelenaEste artigo apresenta o resultado do projeto de intervenção pedagógica denominado “À quarta…família com arte - como potenciar o envolvimento parental no ensino artístico especializado da música”, realizado no Conservatório Regional do Algarve Maria Campina, em 2022.O projeto foi materializado numa série de 10 de aulas de grupo temáticas, nas quais participaram6 alunos de Iniciação ao Violino e respetivos pais/encarregados de educação. Perceber o impacto do envolvimento parental ativo e regulado no desenvolvimento de competências técnico-artísticas e socio emocionais nos alunos participantes foi o objeto de estudo desta investigação. A pesquisa, do tipo naturalista, seguiu uma abordagem metodológica de aproximação à Investigação-Ação (I-A). Como técnicas de recolha e produção de dados recorreu-se à observação direta e participante e notas de campo, registos digitais e audiovisuais, ao feedback qualitativo e aos inquéritos por questionário aplicados aos alunos e encarregados de educação no início e no fim da intervenção pedagógica. A análise estatística e de conteúdo de todos os dados e registos recolhidos pelos diferentes instrumentos e técnicas evidenciaram que, no contexto da iniciação instrumental, o envolvimento parental contribui de forma direta para o desenvolvimento de competências transversais nos alunos, na medida em que estes se sentem mais apoiados e motivados na aprendizagem e, não menos importante, os pais sentem-se valorizados pelos seus filhos na ajuda que se tornaram capazes de dar no estudo em casa.
- a* values to follow lycopene concentration during ripening and storage of tomato (cv. Caruso)Publication . Rocha, A.; Schauwers, A.; Morais, A.