Browsing by Author "Ribeiro, Alessandra Braga"
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- Advances in antitumor effects using liposomal citrinin in induced breast cancer modelPublication . Moura, Michely Laiany Vieira; Menezes, Ag-Anne Pereira Melo de; Filho, José Williams Gomes de Oliveira; Nascimento, Maria Luiza Lima Barreto do; Reis, Antonielly Campinho dos; Ribeiro, Alessandra Braga; Silva, Felipe Cavalcanti Carneiro da; Nunes, Adriana Maria Viana; Rolim, Hercília Maria Lins; Cavalcante, Ana Amélia de Carvalho Melo; Sousa, João Marcelo de Castro eThe study aimed to evaluate the antitumor and toxicogenetic effects of liposomal nanoformulations containing citrinin in animal breast carcinoma induced by 7,12-dimethylbenzanthracene (DMBA). Mus musculus virgin females were divided into six groups treated with (1) olive oil (10 mL/kg); (2) 7,12-DMBA (6 mg/kg); (3) citrinin, CIT (2 mg/kg), (4) cyclophosphamide, CPA (25 mg/kg), (5) liposomal citrinin, LP-CIT (2 μg/kg), and (6) LP-CIT (6 µg/kg). Metabolic, behavioral, hematological, biochemical, histopathological, and toxicogenetic tests were performed. DMBA and cyclophosphamide induced behavioral changes, not observed for free and liposomal citrinin. No hematological or biochemical changes were observed for LP-CIT. However, free citrinin reduced monocytes and caused hepatotoxicity. During treatment, significant differences were observed regarding the weight of the right and left breasts treated with DMBA compared to negative controls. Treatment with CPA, CIT, and LP-CIT reduced the weight of both breasts, with better results for liposomal citrinin. Furthermore, CPA, CIT, and LP-CIT presented genotoxic effects for tumor, blood, bone marrow, and liver cells, although less DNA damage was observed for LP-CIT compared to CIT and CPA. Healthy cell damage induced by LP-CIT was repaired during treatment, unlike CPA, which caused clastogenic effects. Thus, LP-CIT showed advantages for its use as a model of nanosystems for antitumor studies.
- Antibacterial and healing effect of chicha gum hydrogel (sterculia striata) with nerolidolPublication . Lima, Idglan Sá de; Ferreira, Maria Onaira Gonçalves; Barros, Esmeralda Maria Lustosa; Rizzo, Marcia dos Santos; Santos, Jailson de Araújo; Ribeiro, Alessandra Braga; Furtini, Josy Anteveli Osajima; Silva-Filho, Edson C.; Estevinho, Leticia M.Chicha gum is a natural polymer obtained from the Sterculia striata plant. The hydroxyl groups of its structure have a chemical affinity to form hydrogels, which favors the association with biologically active molecules, such as nerolidol. This association improves the biological properties and allows the material to be used in drug delivery systems. Chicha gum hydrogels associated with nerolidol were produced at two concentrations: 0.01 and 0.02 g mL−1. Then, the hydrogels were characterized by thermogravimetry (TG), Fourier Transform Infrared spectroscopy (FTIR), and rheological analysis. The antibacterial activity was tested against Staphylococcus aureus and Escherichia coli. The cytotoxicity was evaluated against Artemia salina. Finally, an in vivo healing assay was carried out. The infrared characterization indicated that interactions were formed during the gel reticulation. This implies the presence of nerolidol in the regions at 3100–3550 cm−1. The rheological properties changed with an increasing concentration of nerolidol, which resulted in less viscous materials. An antibacterial 83.6% growth inhibition effect was observed using the hydrogel with 0.02 g mL−1 nerolidol. The in vivo healing assay showed the practical activity of the hydrogels in the wound treatment, as the materials promoted efficient re-epithelialization. Therefore, it was concluded that the chicha hydrogels have the potential to be used as wound-healing products.
- Are structurally modified galactomannan derivatives biologically active?Publication . Geronço, Maurycyo Silva; Ramos, Igor Frederico da Silveira; Silva Filho, Edson Cavalcanti da; Rizzo, Márcia dos Santos; Ribeiro, Alessandra Braga; Costa, Marcilia Pinheiro daGalactomannans are versatile macromolecules with broad industrial potential. The influence of changes in the chemical structures and respective bioactivities of these polysaccharides have been extensively studied. The derivatives obtained by sulfation, complexation, and phosphorylation are the most studied biological properties in galactomannans. The derivatives obtained have shown several pharmacological activities such as antiviral, antimicrobial, anticoagulant, fibrinolytic, chemopreventive, anticancer, antioxidant, chondroprotective, analgesic, immunomodulatory, and antileishmanial. Considering the relevance of these studies, we aim to provide an overview of studies that apply galactomannan modification or derivatization strategies to improve their properties for applications in the biomedical area. We identified the success of most modified galactomannans for pharmacological purposes. However, some studies found loss of bioactivity of the original polysaccharide after chemical changes to its original structures.
- Avaliação da qualidade microbiológica de gelados comestíveis comercializados por ambulantes em refeitórios de um campus universitário da cidade de Teresina-PiauíPublication . Cruz, Andreson Félix da; Mendes, Ana Victória da Silva; Santos, Andressa Amorim dos; Barros, Izabel Costa; Ribeiro, Alessandra Braga; Albuquerque, Waleska Ferreira deO dindin é um alimento gelado e refrescante, que pode ser classificado quanto a origem da matéria-prima utilizada na produção como natural ou artificial e de acordo com a RDC nº 12, de 02 de janeiro de 2001, a qual estabelece parâmetros microbiológicos para o controle sanitário na área de alimentos, este alimento é classificado como gelado comestível. Este trabalho teve por objetivo avaliar a qualidade microbiológica de gelados comestíveis comercializados em refeitórios de um campus universitário da cidade de Teresina-Piauí. Para tanto, foram coletadas 23 amostras adquiridas de 05 ambulantes, no período de outubro de 2019 a março de 2020. Após a coleta, as amostras foram preparadas para análise pelo método de diluição decimal seriada e forma submetidas em seguida a testes presuntivos e confirmatórios para enumeração de coliformes a 35 °C, de coliformes a 45 °C e pesquisa de Escherichia coli; realizou-se a pesquisa de estafilococos coagulase positiva e teste de presença ou ausência de Salmonella sp. Os resultados sugerem que 56,50% das amostras indicaram contaminação por coliformes a 35 °C e que 43,50% das amostras encontravam-se com contagem de coliformes a 45 °C superior ao estabelecido pela legislação e que não houve indicativo da presença de E. coli em 100% das amostras analisadas. A pesquisa de estafilococos coagulase positiva e Salmonella sp, apresentaram resultados em conformidade com a legislação. Parte das amostras apresentam qualidade microbiológica insatisfatória, sendo necessária a revisão das etapas de produção e armazenamento do produto final quanto ao aspecto sanitário.
- Biocompatible gels of chitosan–buriti oil for potential wound healing applicationsPublication . Ferreira, Maria Onaira Gonçalves; Lima, Idglan Sá; Ribeiro, Alessandra Braga; Lobo, Anderson O.; Rizzo, Marcia S.; Osajima, Josy Anteveli; Silva-Filho, Edson C.The buriti oil (Mauritia flexuosa L.) can be associated with polymeric matrices for biomedical applications. This study aimed to evaluate the e ect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent. The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis. Biological tests including antimicrobial, antioxidant, anti-inflammatory and healing e ects were also investigated. Buriti oil is composed of oleic and palmitic acids, and the main volatile compounds were identified. The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be e cient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL. Similar behavior was observed for antioxidant activity, determined by the -carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals. Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction. The results indicated that the gel chitosan–buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
- Biopolymer from water kefir as a potential clean-label ingredient for health applications: evaluation of new propertiesPublication . Lucena, Monalisa de Alencar; Ramos, Igor Frederico da Silveira; Geronço, Maurycyo Silva; Araújo, Ricardo de; Filho, Francisco Lopes da Silva; Silva, Luís Manuel Lopes Rodrigues da; Sousa, Rayran Walter Ramos de; Ferreira, Paulo Michel Pinheiro; Osajima, Josy Anteveli; Silva-Filho, Edson Cavalcanti; Rizzo, Márcia dos Santos; Ribeiro, Alessandra Braga; Costa, Marcilia Pinheiro daThe present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 x 10(5) Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 degrees C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.
- Biopolymeric materials used as nonviral vectors: a reviewPublication . Santos, Jailson de Araújo; Liarte, Daniel Barbosa; Ribeiro, Alessandra Braga; Rizzo, Marcia dos Santos; Costa, Marcília Pinheiro da; Osajima, Josy A.; Silva-Filho, Edson C.Bacterial transformation and gene transfection can be understood as being the results of introducing specific genetic material into cells, resulting in gene expression, and adding a new genetic trait to the host cell. Many studies have been carried out to investigate different types of lipids and cationic polymers as promising nonviral vectors for DNA transfer. The present study aimed to carry out a systematic review on the use of biopolymeric materials as nonviral vectors. The methodology was carried out based on searches of scientific articles and applications for patents published or deposited from 2006 to 2020 in different databases for patents (EPO, USPTO, and INPI) and articles (Scopus, Web of Science, and Scielo). The results showed that there are some deposits of patents regarding the use of chitosan as a gene carrier. The 16 analyzed articles allowed us to infer that the use of biopolymers as nonviral vectors is limited due to the low diversity of biopolymers used for these purposes. It was also observed that the use of different materials as nonviral vectors is based on chemical structure modifications of the material, mainly by the addition of cationic groups. Thus, the use of biopolymers as nonviral vectors is still limited to only a few polysaccharide types, emphasizing the need for further studies involving the use of different biopolymers in processes of gene transfer.
- Chia seed mucilage (Salvia hispanica L.): an emerging biopolymer for industrial applicationPublication . Ramos, Igor Frederico da Silveira; Lima Fernandes, Valesca; Lucena, Monalisa de Alencar; Geronço, Maurycyo Silva; Costa, Marcília Pinheiro da; Rizzo, Márcia dos Santos; Ribeiro, Alessandra BragaBiopolymers are widely distributed in nature and have a wide variety of biological properties and applications. Chia (Salvia hispanica L.) is an herbaceous plant that belongs to the Lamiaceae family, which when in contact with water exude the fraction of soluble fiber in the form of mucilage. In view of the above, this study aims to provide an overview of the characteristics and applications of chia mucilage that make it a biopolymer of industrial interest. The data collection was carried out in the databases: SciELO (Scientific Electronic Library Online), Science Direct, MDPI, Wiley Online Library, Springer and Periodical capes using the following descriptors: “Chia Seed Mucilage”, “Technological Properties”, “Biological Activities”, and “Applications” in alone and in association. After the critical analysis of the selected articles, it was possible to conclude that the chia mucilage has numerous and unique physical-chemical, chemical and technological properties. Applications in the food field are among the most used in view of the industrial perspective. However, the infinite potential that this biomaterial has for the pharmaceutical and agricultural industries is undeniable.
- Cytotoxic and antibacterial activity of chichá gum hydrogel associated with nerolidolPublication . Lima, Idglan Sá; Gonçalves, Maria Onaira Ferreira; Silva, Albert Santos; Ribeiro, Alessandra Braga; Barreto, Humberto Medeiros; Furtini, Josy Anteveli Osajima; Filho, Edson Cavalcanti da SilvaChichá gum is a polysaccharide from the Sterculia striata plant, with a chemical structure composed of hydroxyl groups, which favor the formation of hydrogels, enabling the association with other materials to improve their biological properties and as drug delivery systems. Hydrogels were produced from chichá gum associated with nerolidol in concentrations of 1 and 2%, cytotoxicity was evaluated against Artemia salina. The hydrogels were tested against the strains Staphylococcus aureus and Escherichia coli, by the direct contact test. the material showed no toxic activity and an antibacterial effect was observed with 83.6% growth inhibition with the use of the hydrogel with 2% nerolidol. It concludes that the materials produced have the potential to be used in the future as products with good antibacterial properties.
- Emerging applications of Sterculia striata gum in medical, pharmaceutical, and environmental fields: prospects and challengesPublication . Freitas, Alessandra Ribeiro; Ribeiro, António José; Santos, Pauline Sousa dos; Oliveira, Thaísa Cardoso de; Oliveira, Jabson Herber Profiro de; Osajima, Josy A.; Ribeiro, Alessandra Braga; Veiga, Francisco; Bezerra, Roosevelt D.S.; Silva, Albert S.; Soares-Sobrinho, José Lamartine; Muniz, Edvani Curti; Silva-Filho, Edson C.Trees of the genus Sterculia produce acidic polysaccharides with high viscosity and the ability to form gels in water. This work emphasized the species Sterculia striata and the uses of its plant derivatives, such as seeds, fruits, stem bark, and exudates. The species showed prospects for industrial application (seeds with high protein content, fruits with antioxidant potential, and stem bark with anti-inflammatory, antinociceptive, and gastroprotective potential). From a physicochemical and rheological point of view, the exudate has a high molar mass value; it contains uronic acid, galactose, rhamnose, and xylose. Sterculia striata gum (SSG) is highly viscous in solution and can form thermoreversible gels where gelation conditions depend on the purification method, acetyl groups, and presence of salt. Due to the presence of carboxylic acid groups, polyanionic behaving SSG allow the formation of polyelectrolyte complexes, which have been used in drug delivery systems (DDS) to encapsulate drugs such as antimalarial drugs, improve essential oil stability and obtain films for antimicrobial purposes. This review contributes to the understanding of the use of this biomaterial, providing a basis for new research on its different applications and industrial use, with a focus on the pharmaceutical, medical, food, and environmental fields.