Browsing by Issue Date, starting with "2023-11-03"
Now showing 1 - 7 of 7
Results Per Page
Sort Options
- Bodycams e segurança pública : a restrição legal de direitos fundamentais e o princípioPublication . Guedes, Patrícia Pereira dos Santos; Calvão, Maria Filipa Pires Urbano da CostaExiste um equilíbrio entre a nova Lei portuguesa sobre bodycams e direitos fundamentais, como a privacidade e a liberdade? Esta é a questão à qual pretendemos responder no final da investigação presente nesta tese. Apesar dos muitos benefícios associados às câmaras corporais, a verdade é que, alguns obstáculos continuam a ser trazidos para discussão no que diz respeito a esta nova categoria de vigilância. As próximas páginas são o resultado de uma investigação que tem por base casos reais, legislação nacional e diferentes pontos de vista, enquanto forma de perceber se a concreta utilização das bodycams, e as últimas medidas adotadas para vigiar cidadãos e agentes responsáveis pela aplicação da lei, são de facto, providas de proporcionalidade.
- Evaluation of microbial behavior in selective culture media for the microbiological analysis for fruit preparationsPublication . Gonçalves, Beatriz Maria Teixeira Costa; Teixeira, Paula Cristina MaiaIn the food industry, when a microbiological analysis is performed, it is generally based on the detection of microorganisms in immunological, visual, or biochemical ways. Nowadays, it is possible to cultivate microorganisms and induce the proliferation of cells by adding sufficient nutrients to the culture media. The study developed had the main objective of developing an optimum culture media for the growth of yeasts, bacteria and moulds. These culture media are intended to mimic the fruit preparations and guarantee that if the samples are contaminated, the microorganism will grow in the media and grow faster and more significantly to identify the microorganism quickly. To achieve this first was made an overview by adding different components to simpler culture media, like NA and PDA, to understand how each component affected the growth of the different microorganisms, being the microorganisms used throughout the study Bacillus cereus, Escherichia coli, Leuconostoc mesenteroides, Pseudomonas aeruginosa, Saccharomyces cerevisiae, Pichia fermentans, Candida intermedia e Neosartorya fischeri. Later, a confirmation was performed to see if, at a statistical and qualitative level, the components negatively or positively affected the growth of the microorganisms. Finally, the components with better results were mixed in the media. The culture media that better enhanced the bacteria growth were 201 and 203, where both sucrose and lysine were added, 201 magnesium and 203 magnesium and iron, once three different bacteria had positive outcomes in their three inoculums for both culture media. For yeasts, of the six media tested, only one stood out, being 601, where fructose, lysine, biotin thiamine, magnesium and irom were incorporated into the medium. This media had a positive result for two yeasts, S. cerevisiae and P. fermentans, and the yeast C. intermedia. The media may not have had a positive outcome but did not have a negative result, which means that it does not negatively compromise the growth of the yeast. The only mould tested was N. fischeri, and it was possible to perceive that it grows better in PDA than in the tested media, so it is possible to assume that simpler culture media, like PDA, are better for growth for this mould. Then was possible to conclude that the combination of certain components, when added to simpler media, made the bacteria and yeasts colonies to grow larger. The opposite was observed in the case of the mould, in which its colonies grew larger in simpler media.
- The National Institute of Child Health and Human Development ProtocolPublication . Myklebust, Trond; La Rooy, David J.; Peixtoto, Carlos E.
- Responsabilidade dos administradores e dever de diligência em matéria de sustentabilidadePublication . Afonso, Maria Inês Belo Grácio; Antunes, Ana Filipa dos Santos MoraisA presente dissertação incide sobre a responsabilidade empresarial e o dever de diligência, tendo por referência a Resolução do Parlamento Europeu, de 10 de março de 2021, em matéria de responsabilidade empresarial e dever de diligência das empresas, bem como a Proposta de Diretiva, de 23 de fevereiro de 2022, relativa ao dever de diligência das empresas em matéria de sustentabilidade e, ainda, a Orientação Geral do Conselho da União Europeia, adotada em 1 de dezembro de 2022, sobre a Proposta de Diretiva do Parlamento Europeu e do Conselho, relativa ao dever de diligência das empresas em matéria de sustentabilidade. Pretendemos incidir na Orientação Geral do Conselho da União Europeia, procurando refletir sobre o respetivo âmbito de aplicação e estabelecer uma ponte com os artigos 64.º e 72.º do Código das Sociedades Comerciais (CSC), que regulam os deveres fundamentais dos administradores e a responsabilidade de membros da administração para com a sociedade, respetivamente. Começaremos por estudar o regime europeu proposto enquanto modelo de conduta, obrigatório e harmonizado, aprofundando o panorama de um “caminho europeu sustentável” para as empresas. De seguida, propomo-nos analisar os preceitos nacionais supramencionados. Primeiramente, cumpre confrontar o dever de diligência previsto na Orientação Geral do Conselho Europeu da União Europeia com aquele que é já estatuído no texto do artigo 64.º do CSC. A mesma linha de raciocínio servirá para comparar a responsabilidade prevista na iniciativa europeia com o preceituado no artigo. 72.º, n.º 2 do CSC. Finalmente, analisaremos a questão que se impõe: Será este modelo europeu tão inovador comparativamente ao regime previsto a nível nacional?
- Sugarcane light-colored lignin: a renewable resource for sustainable beautyPublication . Mota, Inês F.; Antunes, Filipa; Fangueiro, Joana F.; Costa, Carina A. E.; Rodrigues, Alírio E.; Pintado, Manuela E.; Costa, Patrícia S.Lignin has emerged as a promising eco-friendly multifunctional ingredient for cosmetic applications, due to its ability to protect against ultraviolet radiation and its antioxidant and antimicrobial properties. However, its typical dark color and low water solubility limit its application in cosmetics. This study presents a simple process for obtaining light-colored lignin (LCLig) from sugarcane bagasse (SCB) alkaline black liquor, involving an oxidation treatment with hydrogen peroxide, followed by precipitation with sulfuric acid. The physico-chemical characterization, antioxidant and emulsifying potential of LCLig, and determination of its safety and stability in an oil-in-water emulsion were performed. A high-purity lignin (81.6%) with improved water solubility was obtained, as a result of the balance between the total aromatic phenolic units and the carboxylic acids. In addition, the antioxidant and emulsifying capacities of the obtained LCLig were demonstrated. The color reduction treatment did not compromise the safety of lignin for topical cosmetic applications. The emulsion was stable in terms of organoleptic properties (color, pH, and viscosity) and antioxidant activity over 3 months at 4, 25, and 40 °C.
- Characterization of the toxigenic potential of bacillus cereus sensu lato isolated from raw berries and their productsPublication . Oliveira, Márcia; Carvalho, Marta; Teixeira, PaulaBacillus cereus is estimated to be responsible for 1.4–12% of all food poisoning outbreaks worldwide. The objective of this study was to investigate the toxigenic potential of 181 isolates of B. cereus previously recovered from different types of berries and berry products (strawberries, raspberries, blackberries, and blueberries) by assessing the presence of enterotoxin genes (hblA, hblC, hblD, nheA, nheB, nheC, and cytK) and an emetic toxin cereulide synthetase gene (ces). The cytotoxic activity on Caco-2 cells was also evaluated for the two isolates containing the gene cytK. Twenty-three toxigenic profiles were found. The nheABC (91.7%) and hblACD (89.0%) complexes were the most prevalent among the isolates, while the cytK and ces genes were detected in low percentages, 1.1% and 3.3%, respectively. In addition, the nheABC/hblACD complex and ces genes were detected in isolates recovered throughout the production process of blackberries and strawberries. The cytotoxic activity on Caco-2 cells was also observed to be greater than 60% for isolates containing the cytK gene.
- Factors influencing interactions with recruitment chatbotsPublication . Rodelo Diaz, Daniela Cristina; Elmashhara, Maher Georges; Silva, Susana Cristina Lima da Costa eChatbots are a rapidly developing service technology that can be used in various services, such as recruitment. However, little is known about how chatbots might affect candidates' user experience, and several gaps warrant further research. This paper aims to examine the effects of ease to use, usefulness, social presence, trust, and enjoyment on user attitude, satisfaction, and recruiter credibility. To test the proposed model, we designed a recruitment chatbot and conducted a survey-based study. The results of this study suggest that trust and enjoyment are the main drivers of recruitment chatbot use. The findings also show that attitude mediates the relationship between trust and enjoyment on the one hand, and satisfaction and both dimensions of credibility (trustworthiness and attractiveness) on the other. Based on these findings, contributions to theory and practice are discussed.