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- Isolation of several bacterial species from seafood and their inhibition by different natural edible compoundsPublication . Rezende, Lourenço; Barbosa, Joana; Teixeira, PaulaSeafood is a great source of nutrients, indispensable to a healthy diet, ranging from proteins and fatty acids to vitamins and minerals. Due to their nutritional characteristics and composition, seafood products are highly perishable foods. The application of non-toxic, natural, and edible preservatives as a coating to extend the shelf life and inhibit bacterial proliferation of several foods has been a hot topic. This work aimed to perform the microbiological characterization of squids and shrimps skewers during their shelf-life and to evaluate the susceptibility of isolated microorganisms to several natural edible compounds. One hundred bacterial isolates were identified through rRNA 16s sequencing resulting in the detection of several Enterobacteriaceae (Serratia spp., Klebsiella spp., Hafnia spp., Rahnella spp., among others), Pseudomonas spp. and Lactic Acid Bacteria (Lactococcus spp., Leuconostoc spp. and Weissella spp.). No Escherichia coli or Listeria monocytogenes were detected on the analyzed samples. The antibacterial activity of Carvacrol, Olive Leaf Extract, Limonene, different Chitosans, Ethanolic Propolis Extracts, and Citrox® was evaluated by the agar diffusion method against the 100 isolates identified. Minimum inhibitory concentration was determined only for Citrox® (1.69% v/v) since only this compound could inhibit all the isolates. Although preliminary, this study showed evidence that Citrox® has the potential to be used as a natural preservative in these seafood products, improving food safety while reducing waste. However, further studies are required, such as the development of a Citrox®-based coating and its application on this matrix to validate its antimicrobial effect.
- Serious game design and clinical improvement in physical rehabilitation: systematic reviewPublication . Vieira, Catarina; Pais-Vieira, Carla Ferreira da Silva; Novais, João; Perrotta, AndréBackground: Serious video games have now been used and assessed in clinical protocols, with several studies reporting patient improvement and engagement with this type of therapy. Even though some literature reviews have approached this topic from a game perspective and presented a broad overview of the types of video games that have been used in this context, there is still a need to better understand how different game characteristics and development strategies might impact and relate to clinical outcomes. Objective: This review assessed the relationship between the characteristics of serious games (SGs) and their relationship with the clinical outcomes of studies that use this type of therapy in motor impairment rehabilitation of patients with stroke, multiple sclerosis, or cerebral palsy. The purpose was to take a closer look at video game design features described in the literature (game genre [GG], game nature [GN], and game development strategy [GDS]) and assess how they may contribute toward improving health outcomes. Additionally, this review attempted to bring together medical and game development perspectives to facilitate communication between clinicians and game developers, therefore easing the process of choosing the video games to be used for physical rehabilitation. Methods: We analyzed the main features of SG design to obtain significant clinical outcomes when applied to physical rehabilitation of patients recovering from motor impairments resulting from stroke, multiple sclerosis, and cerebral palsy. We implemented a PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) database-adjusted electronic search strategy for the PubMed, IEEE Xplore, and Cochrane databases. Results: We screened 623 related papers from 2010-2021 and identified 12 that presented results compatible with our inclusion criteria. A total of 512 participants with stroke (8 studies, 417 participants), cerebral palsy (1 study, 8 participants), and multiple sclerosis (2 studies, 46 participants) were included; 1 study targeting the elderly (41 participants) was also included. All studies assessed motor, sensory, and functional functions, while some also measured general health outcomes. Interventions with games were used for upper-limb motor rehabilitation. Of the 12 studies, 8 presented significant improvements in at least one clinical measurement, of which 6 presented games from the casual GG, 1 combined the casual, simulation, and exergaming GGs, and 2 combined the sports and simulation GGs. Conclusions: Of the possible combinations of game design features (GG, GN, and GDS) described, custom-made casual games that resort to the first-person perspective, do not feature a visible player character, are played in single-player mode, and use nonimmersive virtual reality attain the best results in terms of positive clinical outcomes. In addition, the use of custom-made games versus commercial off-the-shelf games tends to give better clinical results, although the latter are perceived as more motivating and engaging.
- Aplicação de uma estratégia omnichannel na Parques de SintraPublication . Carvalho, Sara Maria Pinheiro de; Celeste, Pedro Manuel Amador RodriguesEm 2020 a crise pandémica que varreu o mundo acelerou a presença das marcas e dos consumidores no digital. A implementação de estratégias omnichannel, com integração dos canais de venda e comunicação para uma experiência de consumo contínua e uniforme, determina mais do que nunca o futuro de muitas empresas. Para além de ir ao encontro das expectativas do consumidor e de isso ter um impacto directo nas vendas, a implementação de uma estratégia omnichannel permite a recolha de dados fundamentais para as decisões de gestão. Tal é válido nas mais variadas áreas de actividade e o turismo cultural, em que se enquadra a Parques de Sintra, não é excepção. Assim, neste trabalho de investigação e dissertação analisa-se a aplicabilidade de uma estratégia omnichannel na Parques de Sintra.
- A responsabilidade civil das agências de notação de risco de critério perante terceiros investidoresPublication . Gonzalez, Susana Raquel Marques; Gomes, José João Ferreira
- Reiniciar a escola para a era digitalPublication . Andrade, António
- The INCREASE project: intelligent collections of food-legume genetic resources for European agrofood systemsPublication . Bellucci, Elisa; Aguilar, Orlando Mario; Alseekh, Saleh; Bett, Kirstin; Brezeanu, Creola; Cook, Douglas; Rosa, Lucía de la; Delledonne, Massimo; Dostatny, Denise F.; Ferreira, Juan J.; Geffroy, Valérie; Ghitarrini, Sofia; Kroc, Magdalena; Agrawal, Shiv Kumar; Logozzo, Giuseppina; Marino, Mario; Mary-Huard, Tristan; McClean, Phil; Meglic, Vladimir; Messer, Tamara; Muel, Frédéric; Nanni, Laura; Neumann, Kerstin; Servalli, Filippo; Strajeru, Silvia; Varshney, Rajeev K.; Vasconcelos, Marta W.; Zaccardelli, Massimo; Zavarzin, Aleksei; Bitocchi, Elena; Frontoni, Emanuele; Fernie, Alisdair R.; Gioia, Tania; Graner, Andreas; Guasch, Luis; Prochnow, Lena; Oppermann, Markus; Susek, Karolina; Tenaillon, Maud; Papa, RobertoFood legumes are crucial for all agriculture-related societal challenges, including climate change mitigation, agrobiodiversity conservation, sustainable agriculture, food security and human health. The transition to plant-based diets, largely based on food legumes, could present major opportunities for adaptation and mitigation, generating significant co-benefits for human health. The characterization, maintenance and exploitation of food-legume genetic resources, to date largely unexploited, form the core development of both sustainable agriculture and a healthy food system. INCREASE will implement, on chickpea (Cicer arietinum), common bean (Phaseolus vulgaris), lentil (Lens culinaris) and lupin (Lupinus albus and L. mutabilis), a new approach to conserve, manage and characterize genetic resources. Intelligent Collections, consisting of nested core collections composed of single-seed descent-purified accessions (i.e., inbred lines), will be developed, exploiting germplasm available both from genebanks and on-farm and subjected to different levels of genotypic and phenotypic characterization. Phenotyping and gene discovery activities will meet, via a participatory approach, the needs of various actors, including breeders, scientists, farmers and agri-food and non-food industries, exploiting also the power of massive metabolomics and transcriptomics and of artificial intelligence and smart tools. Moreover, INCREASE will test, with a citizen science experiment, an innovative system of conservation and use of genetic resources based on a decentralized approach for data management and dynamic conservation. By promoting the use of food legumes, improving their quality, adaptation and yield and boosting the competitiveness of the agriculture and food sector, the INCREASE strategy will have a major impact on economy and society and represents a case study of integrative and participatory approaches towards conservation and exploitation of crop genetic resources.
- Isolation of several bacterial species from seafood and their inhibition by different natural edible compoundsPublication . Rezende, Lourenço; Barbosa, Joana; Teixeira, Paula
- Judicialization of economic and monetary union: between a rock and a soft place?Publication . Matos, Nuno AlbuquerqueThe European Central Bank has been active since the sovereign debt crisis that struck European Union Member States by putting in place several asset-purchasing programmes such as Outright Monetary Transactions and Public Sector Purchase Programme. As much as these decisions have proven the pivotal importance of this institution within the monetary union, they have also spurred controversy on potentially having exceeded the competences attributed to the Union. The german federal constitutional court heard challenges to both and requested the Court of Justice to decide on their validity within the framework of a preliminary ruling. The decision of the former court to declare the Public Sector Purchase Programme ultra vires -in this way countering the preliminary ruling decision- as well as its argumentation could produce many institutional consequences to both the European Central Bank and Court of Justice of the European Union. Finally, it has shown the limits of European Union integration and will inevitably propel discussions on which way to go in the future: It is time for this discussion to come out from courtrooms into the public sphere.
- O problema do tipo legal de violência doméstica : a exigência de coabitação em caso de vítima vulnerável em razão da idade avançadaPublication . Castro, Inês Rocha de; Ferreira, Maria Elisabete da CostaA presente Dissertação tem como objetivo o estudo do enquadramento legal das pessoas idosas vítimas do crime de violência doméstica, através de uma análise crítica ao requisito necessário de coabitação entre a vítima idosa e o agente do crime. Ao procurarmos definir, num primeiro momento, o que é ser especialmente vulnerável em razão da idade avançada aos olhos da lei penal em Portugal, passamos a analisar a al. d) do nº1 do art. 152.º do CP, nomeadamente a obrigatoriedade de coabitação entre o idoso e o seu agressor. Pretendemos, com este estudo, demonstrar a ainda diminuta proteção penal dos nossos idosos vítimas de violência doméstica, partindo do requisito da coabitação, exigido apenas no caso de vítimas particularmente indefesas, como um pressuposto criador de fragilidades para os idosos vítimas de violência pelos seus familiares, conduzindo-os a uma dupla vitimização.
- Pornografia de menores : o conceito de representação realista de menor e a (i)legitimidade da incriminaçãoPublication . Rodrigues, Cristiano Filipe Silva; Freitas, Pedro Miguel FernandesA presente dissertação pretende promover a reflexão crítica sobre um dos temas mais complexos do Direito Penal: os limites à incriminação da pornografia de menores. Primeiro, relembramos todos os conceitos inerentes às finalidades da intervenção penal, desde a noção de bem jurídico à relação entre política-criminal e Constituição. Depois, procedemos a uma evolução histórica da legislação em matéria de criminalidade sexual, uma vez que aqui se deram as mais importantes transformações a respeito do bem jurídico objeto de tutela. Uma vez compreendido o enquadramento atual, analisamos o crime de pornografia de menores. Trataremos o conceito de pornografia infantil e as controvérsias geradas por algumas das condutas típicas. Por fim, dedicamos uma especial atenção à pseudopedopornografia, onde se questiona um possível retorno à moral sexual e os eventuais conflitos com outros direitos fundamentais.