Browsing by Author "Souza, Evandro Leite de"
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- Addition of probiotic bacteria in a semi-hard goat cheese (coalho): survival to simulated gastrointestinal conditions and inhibitory effect against pathogenic bacteriaPublication . Oliveira, Maria Elieidy Gomes de; Garcia, Estefânia Fernandes; Oliveira, Carlos Eduardo Vasconcelos de; Gomes, Ana Maria Pereira; Pintado, Maria Manuela Esteves; Madureira, Ana Raquel Mendes Ferreira; Conceição, Maria Lúcia da; Queiroga, Rita de Cássia Ramos do Egypto; Souza, Evandro Leite deIn this study, the survival of the probiotics Lactobacillus acidophilus (LA-5), Lactobacillus casei subsp. paracasei (L. casei 01) and Bifidobacterium lactis (BB12) incorporated in a Brazilian semi-hard goat cheese (coalho) when exposed to in vitro simulated conditions of digestion was assessed. The inhibitory effects of these probiotic bacteriawere also evaluated against Listeriamonocytogenes and Staphylococcus aureus in the goat coalho cheese during refrigerated storage. At the end of the in vitro digestion, all of the probiotic tested strains presented decreased (p b 0.05) viable cell counts (5.5–6.0 log cfu/g) with respect to those determined before exposure to the mouth conditions (7–8 log cfu/g). L. casei subsp. paracasei presented inhibition rate of 7.87% and 23.63% against S. aureus on the 14th and 21st day of storage at 10 °C, respectively; against L. monocytogenes these values were 12.96 and 32.99%. Positive inhibition rates of B. lactis toward S. aureus were found on the 1st, 14th and 21st days of storage (16.32%, 10.12% and 3.67%, respectively); and against L. monocytogenes only on the 1st day of storage (3.28%). From these results, goat coalho cheese could be an interesting carrier of probiotic strains of L. acidophilus, L. casei subsp. paracasei and B. lactis. Moreover, L. casei subsp. paracasei, could be used as protective culture for delaying the growth of S. aureus and L. monocytogenes in goat coalho cheese.
- Chitosan produced from Mucorales fungi using agroindustrial by-products and its efficacy to inhibit Colletotrichum speciesPublication . Bergera, Lúcia Raquel Ramos; Stamford, Thayza Christina Montenegro; Oliveira, Kataryne Árabe Rimáde; Pessoa, Adjanede Miranda Pereira; Lima, Marcos Antonio Barbosa de; Pintado, Maria Manuela Estevez; Câmara, Marcos Paz Saraiva; Franco, Lucianade Oliveira; Souza, Marciane Magnania Evandro Leite de; Souza, Evandro Leite deThis study evaluated corn steep liquor (CSL) and papaya peel juice (PPJ) in mixture as substrates for thecultivation (96 h, 28◦C, pH 5.6, 150 rpm) of Mucorales fungi for chitosan production, and determinedthe growth-inhibitory effect of the fungal chitosan (FuCS) obtained under optimized conditions againstphytopathogenic Colletotrichum species. All Mucorales fungi tested were capable of growing in CSL-PPJmedium, showing FuCS production in the range of 5.02 (Fennelomyces heterothalicus SIS 28) − 15.63 mg/g(Cunninghamella elegans SIS 41). Highest FuCS production (37.25 mg/g) was achieved when C. eleganswas cultivated in medium containing 9.43% CSL and 42.5% PPJ. FuCS obtained under these conditionsshowed a deacetylation degree of 86%, viscosity of 120 cP and molecular weight of 4.08 × 104g/mol.FuCS at 5000, 7500 and 10,000 ppm inhibited the growth of all Colletotrichum species tested. FuCS alsoinduced alterations in the morphology of C. fructicola hyphae. CSL-PPJ mixtures are suitable substratesfor the cultivation of Mucorales fungi for FuCS production. Chitosan from C. elegans cultivated in CSL-PPJmedium is effective in inhibiting phytopathogenic Colletotrichum species.
- Effect of supplementation with probiotic lactic acid bacteria, separately or combined, on acid and sugar production in goat ‘coalho’ cheesePublication . Bezerra, Taliana Kênia Alves; Arcanjo, Narciza Maria de Oliveira; Garcia, Estefânia Fernandes; Gomes, Ana Maria Pereira; Queiroga, Rita de Cássia Ramos do Egypto; Souza, Evandro Leite de; Madruga, Marta SuelyThis study analyzed the glycolytic effects of adding isolated and combined probiotic bacterial strains to goat ‘coalho’ cheese. The cheeses were: C-with added Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris; L-with added Lactobacillus acidophilus; P-with added Lactobacillus paracasei; B-with added Bifidobacterium lactis; and M, added a “mixed” culture with the three probiotic. Based on the results, the different probiotic lactic acid and the length of storage were verified as factors affecting the organic and fatty acids profiles. Probioticlactic acid bacteria exhibited higher glycolytic potential throughout the 28- day storage, leading to high sugar consumption and organic acid production, with the consequent formation of a more intense cheese aroma. When evaluating the fatty acid profile, the addition of the B. lactis culture and of the mixed culture was observed to have a positive effect, providing a fatty acid profile with better nutritional value, i.e., with a higher of conjugated linoleic acids and unsaturated fatty acids.
- Effects of added Lactobacillus acidophilus and Bifidobacterium lactis probiotics on the quality characteristics of goat ricotta and their survival under simulated gastrointestinal conditionsPublication . Meira, Quênia Gramile Silva; Magnan, Marciane; Medeiros Júnior, Francisco Cesino de; Queiroga, Rita de Cássia Ramos do Egito; Madruga, Marta Suely; Gullón, Beatriz; Gomes, Ana Maria; Pintado, Maria Manuela Estevez; Souza, Evandro Leite deThis study evaluated the effects of incorporating the probiotics Bifidobacterium animalis subsp. lactis Bb-12 (B. lactis) or Lactobacillus acidophilus La-05 (L. acidophilus) into goat ricotta on the technological, physicochemical, physical and sensory parameters of this product during refrigerated storage, as well as the protective effects of the goat ricotta on the survival of the tested probiotics during exposure to simulated gastrointestinal conditions. Incorporating the tested probiotics did not affect the yield or syneresis of the obtained goat ricotta. The counts of L. acidophilus and B. lactis during the chosen storage period were approximately 6 log CFU/g. The ricotta samples containing a probiotic strain presented smaller and greater amounts of lactose and lactic acid, respectively, and exhibited greater hardness and lower brightness after storage compared with the samples lacking a probiotic. No differences were observed in the fatty acid profiles of the goat ricotta containing or not containing a probiotic. All of the ricotta samples were described as a soft cheese with a homogeneous texture; however, the goat ricotta cheeses containing L acidophilus or B. lactis were described as having a more acidic flavor. At the end of a challenge using experimental human digestive conditions, the counts of each of the tested probiotic strains were approximately 6 log CFU/g if it had been incorporated into goat ricotta. These results demonstrated the feasibility of incorporating L. acidophilus or B. lactis into goat ricotta because these probiotics did not negatively affect the quality characteristics of this product and suggested that goat ricotta is an efficacious food matrix for maintaining the viability of these probiotics during storage and under the stressful conditions imposed by the human gastrointestinal tract. (C) 2015 Elsevier Ltd. All rights reserved.
- Impact of honey on quality characteristics of goat yogurt containing probiotic Lactobacillus acidophilusPublication . Machado, Tamires Alcântara Dourado Gomes; Oliveira, Maria Elieidy Gomes de; Campos, Maria Isabel Ferreira; Assis, Paloma Oliveira Antonino de; Souza, Evandro Leite de; Madruga, Marta Suely; Pacheco, Maria Teresa Bertoldo; Pintado, Maria Manuela Estevez; Queiroga, Rita de Cássia Ramos do EgyptoThis study aimed to analyze the influence of adding stingless bee honey (produced by Melipona scutellaris Latrelle - uruçu) on the technological, physicochemical and sensory characteristics of goat yogurt containing probiotic Lactobacillus acidophilus La-05 during 28 days of refrigerated storage. Four formulations of goat yogurt were prepared, each varying in the added stingless bee honey amount [(0%, 5%, 10% and 15% (v/v)], but all inoculated with the probiotic L. acidophilus La-05 (0.1 g/L of goat milk). The incorporation of stingless bee honey positively affected several characteristics in goat yogurt containing L. acidophilus La-05, namely the color, syneresis, viscosity, sensory acceptance and purchase intention. All yogurt formulations presented counts of L. acidophilus La-05 above 6.0 log cfu/g by 28 days of storage, but the presence of honey increased the counts (ca. 1 log cfu/g) of L. acidophilus La-05 and yogurt starter bacteria until 21 days of storage. The results of this study presented a successful incorporation of both the probiotic L. acidophilus La-05 and the honey produced by a native Brazilian stingless bee as ingredients of a new goat dairy product with satisfactory nutritional and sensory quality and added market value because of its potential functional properties.
- Ingredients from integral valorization of Isabel grape to formulate goat yogurt with stimulatory effects on probiotics and beneficial impacts on human colonic microbiota in vitroPublication . Silva, Francyeli Araújo; Queiroga, Rita de Cássia Ramos do Egypto; Souza, Evandro Leite de; Voss, Glenise Bierhalz; Pintado, Maria Manuela Estevez; Vasconcelos, Margarida Angélica da SilvaIsabel grape (IG) products have high contents of phenolic compounds and fiber recognized for their positive impacts on microorganisms associated with health benefits to host. This study evaluated the effects of goat yogurts formulated with ingredients from IG integral valorization on the growth and metabolism of different probiotic strains, as well as on the population of selected bacterial groups and metabolic activity of human colonic microbiota in vitro. Goat yogurts with IG ingredients (IGI) stimulated the growth of tested Lactobacillus and Bifidobacterium probiotic strains during a 48-h cultivation, as well as decreased the pH values and enhanced the organic acid production. Goat yogurts with IGI increased the population of Lactobacillus spp. and Bifidobacterium spp. during a 24-h in vitro colonic fermentation. A stable Firmicutes:Bacteroidetes ratio close to 1 was found in media with goat yogurt formulations during the colonic fermentation, being similar to the effect caused by fructooligosaccharides. Goat yogurt formulations with IGI caused increased production of short-chain fatty acids and sugar consumption during colonic fermentation. Goat yogurts with IGI should be a valuable strategy for development of novel added-value foods with beneficial effects on gut microbiota and human health.
- Nutritional, textural and sensory properties of Coalho cheese made of goats', cows' milk and their mixturePublication . Queiroga, Rita de Cássia Ramos do Egypto; Santos, Bárbara Melo; Gomes, Ana Maria Pereira; Monteiro, Maria João; Teixeira, Susana Maria; Souza, Evandro Leite de; Pereira, Carlos José Dias; Pintado, Maria Manuela EstevezThis study assesses and compares the nutritional, textural and sensory characteristics of Coalho cheese made from goat's (CGM) or cow's milk (CCM) and their mixture (CCGM) during cold storage for 28 days. Among the assessed physiochemical parameters, the type of milk used during production only influenced (P < 0.05) the moisture, fat and salt contents of the cheeses. CGM and CCGM showed higher (P < 0.05) content of short- and medium-chain fatty acids, such as C6, C8 and C10 and 02, and long-chain polyunsaturated fatty acids C18:2n6c, and lower content of 06 and C16:1. All cheeses presented satisfactory sensory characteristics for most of the assessed parameters. However the addition of cow's milk to goat's milk improved sensory acceptability, mainly through the reduction of goat's milk odor and flavor. Coalho cheese made with the mixture of cow's and goat's milk maintained particular positive nutritional characteristics of goat's cheese, especially with respect to the fatty acids profile, with improved acceptability. All the cheeses maintained, in general, their properties throughout storage time.
- Polyphenolic profile and antioxidant and antibacterial activities of monofloral honeys produced by Meliponini in the Brazilian semiarid regionPublication . Sousa, Janaína Maria; Souza, Evandro Leite de; Marques, Gilmardes; Meireles, Bruno; Cordeiro, Ângela Tribuzy de Magalhães; Gullón, Beatriz; Pintado, M. E.; Magnani, MarcianeThis study assessed the polyphenolic profile and the antioxidant and antibacterial activities ofmonofloral honeys produced by Meliponini in the Brazilian semiarid region. Honeys from Ziziphus joazeiro Mart. (juazeiro) and Croton heliotropiifolius Kunth (velame branco) showed the highest total phenolic contents (TPCs) and the greatest antioxidant activity in assays with DPPH and ABTS•+ radicals. Honeys from Mimosa quadrivalvis L. (malícia) presented the strongest anti-peroxyl activity in ORAC assay. Juazeiro honeys showed the highest quantities of trans-cinnamic, p-coumaric, ellagic and ferulic acids, as well as of catechin, rutin, hesperetin and chrysin when compared to the other honeys produced by the same bee species. Malícia honeys showed the greatest quantities of myricetin, quercetin and kaempferol among the studied honeys. Honeys with the highest TPCs presented the highest antimicrobial activity. The results showed the impact of the floral source on the polyphenolic profile as well as on the antioxidant and antimicrobial properties of Meliponini honeys.
- Potential prebiotic properties of cashew apple (Anacardium occidentale L.) agro-industrial byproduct on Lactobacillus speciesPublication . Duarte, Francisca Nayara Dantas; Rodrigues, Jéssica Bezerra; Lima, Maiara da Costa; Lima, Marcos dos Santos; Pacheco, Maria Teresa Bertoldo; Pintado, Maria Manuela Estevez; Aquino, Jailane de Souza; Souza, Evandro Leite deThe prebiotic effects of a cashew apple (Anacardium occidentale L.) agro-industrial byproduct powder (CAP) on different potentially probiotic Lactobacillus strains, namely Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Lactobacillus paracasei L-10, were assessed using in vitro experimental models. Accordingly, the growth of the Lactobacillus strains when cultivated in a broth containing CAP (20 or 30 g L-1 ), glucose (20 g L-1 ) or fructooligosaccharides (FOS) (20 g L-1 ) was monitored over 48 h; the prebiotic activity scores of CAP were determined; and the changes in pH values, production of organic acids and consumption of sugars in growth media were verified.
- Sugar profile, physicochemical and sensory aspects of monofloral honeys produced by different stingless bee species in Brazilian semi-arid regionPublication . Sousa, Janaína Maria Batista de; Souza, Evandro Leite de; Marques, Gilmardes; Benassi, Marta de Toledo; Gullón, Beatriz; Pintado, M. E.; Magnani, MarcianeMonofloral honeys produced by the stingless bee M. Subnida Duke and Melipona scutellaris Latrelle in a Brazilian semi-arid region were analyzed regarding their physicochemical (moisture, protein, proline, hydroxymethyl-furfural, color, electrical conductivity, pH, free acidity, and sugar profile) and sensory aspects (color, viscosity and flavor). The floral source influenced the color, acidity, sugar profile, ash and proline content in the honeys. The intensity of the acidic flavor, sweet taste and color in the honeys varied according to the floral source. No differences were perceived for honeys from the same floral source produced by different bee species. Principal component analysis revealed that most of the variability was defined by the water content, total acidity, glucose, sucrose and color, as well as by acid taste, acid flavor, honey flavor and sweet taste. These findings reveal that Brazilian monofloral honeys produced by stingless bees possess well-defined characteristics that are influenced by the floral source.