Percorrer por autor "Silva, Marta Nunes da"
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- Antimicrobial potential of six plant essential oils against Pseudomonas syringae pv. actinidiae: in vitro activity and in planta efficacy do not always alignPublication . Silva, Marta Nunes da; Santos, Miguel G.; Vasconcelos, Marta W.; Carvalho, Susana M. P.Plant essential oils (EOs) are attracting interest as ecofriendly alternatives to antibiotics and copper-based control of kiwifruit bacterial canker (KBC), caused by Pseudomonas syringae pv. actinidiae (Psa). This study chemically profiled six EOs (anise, basil, cardamom, cumin, fennel, and laurel) and evaluated their antimicrobial activity both in vitro and in planta. The in vitro assay targeted four strains, two of Psa and two of the low-virulent P. syringae pv. actinidifoliorum (Pfm), whereas the in planta assay focused on the highly virulent Psa7286 strain, assessed under preventive and curative application regimes (i.e., 14 days pre- or post-inoculation, respectively). Cumin, with cuminaldehyde as its major component (48%), was the most effective EO in vitro, significantly inhibiting growth at 5–10% concentration, whereas anise, rich in anethole (89%), was consistently the least effective one. However, the in planta application of the EOs produced antimicrobial effects that differed markedly from in vitro results and showed strong dependence on the timing of application. Preventive treatment significantly reduced Psa endophytic populations in basil (70%), anise (54%), laurel (42%), and cumin (35%) compared to untreated plants. In contrast, when the EOs were applied post-inoculation (curative treatment), a significant decrease in Psa colonization was observed in laurel (81%), cardamon (70%), cumin (31%) and fennel (29%). Although plant EOs are gaining momentum in the control of Psa and other diseases, translation from in vitro to in planta efficacy is not direct and is strongly timing-dependent, which underscores the need to perform validation trials in planta and to fine-tune application schedules for the integrated management of KBC.
- Assessing the effectiveness of different Pseudomonas syringae pv. actinidiae pre-inoculation procedures to promoting disease visual symptoms in adult kiwifruit plantsPublication . Santos, Miguel G.; Silva, Marta Nunes da; Gallego, Pedro P.; Vasconcelos, Marta W.; Carvalho, Susana M. P.
- Cancro bacteriano do kiwi: novas estratégias de mitigação para um velho problemaPublication . Silva, Marta Nunes daEste trabalho, realizado no âmbito de uma tese de doutoramento levada a cabo na Faculdade de Ciências da Universidade do Porto, conclui que a fertilização azotada à base de nitrato pode ser uma ferramenta útil para aumentar a tolerância das plantas de kiwi contra a bactéria Pseudomonas syringae pv. Actinidiae (Psa) e que alguns óleos essenciais de plantas têm potencial para inclusão numa estratégia mais sustentável de gestão da doença.
- Chitosan increases Pinus pinaster tolerance to the pinewood nematode (Bursaphelenchus xylophilus) by promoting plant antioxidative metabolismPublication . Silva, Marta Nunes da; Santos, Carla S.; Cruz, Ana; López-Villamor, Adrián; Vasconcelos, Marta W.The pine wilt disease (PWD), for which no effective treatment is available at the moment, is a constant threat to Pinus spp. plantations worldwide, being responsible for significant economic and environmental losses every year. It has been demonstrated that elicitation with chitosan increases plant tolerance to the pinewood nematode (PWN) Bursaphelenchus xylophilus, the causal agent of the PWD, but the biochemical and genetic aspects underlying this response have not been explored. To understand the influence of chitosan in Pinus pinaster tolerance against PWN, a low-molecular-weight (327 kDa) chitosan was applied to mock- and PWN-inoculated plants. Nematode population, malondialdehyde (MDA), catalase, carotenoids, anthocyanins, phenolic compounds, lignin and gene expression related to oxidative stress (thioredoxin 1, TRX) and plant defence (defensin, DEF, and a-farnesene synthase, AFS), were analysed at 1, 7, 14, 21 and 28 days post-inoculation (dpi). At 28 dpi, PWN-infected plants elicited with chitosan showed a sixfold lower nematode population when compared to non-elicited plants. Higher levels of MDA, catalase, carotenoids, anthocyanins, phenolic compounds, and lignin were detected in chitosan-elicited plants following infection. The expression levels of DEF gene were higher in elicited plants, while TRX and AFS expression was lower, possibly due to the disease containment-effect of chitosan. Combined, we conclude that chitosan induces pine defences against PWD via modulation of metabolic and transcriptomic mechanisms related with plant antioxidant system.
- Common bean (phaseolus vulgaris l.) mineral profile is negatively affected by elevated atmospheric carbon dioxide (CO2)Publication . Duarte, Rafael D. C.; Silva, Marta Nunes da; Quirós-Vargas, Juan; Muller, Onno; Vasconcelos, Marta W.
- Common bean (Phaseolus vulgaris L.) nutritional profile & soil microbiome community is affected by elevated atmospheric carbon dioxide (CO2)Publication . Duarte, Rafael D. C.; Silva, Marta Nunes da; Quirós-Vargas, Juan; Muller, Onno; Vasconcelos, Marta W.
- Comparative environmental and nutritional sustainability analysis of kabuli and desi chickpea (cicer arietinum l.) types at the farm and product levelPublication . Silva, Beatriz Q.; Silva, Marta Nunes da; Smetana, Sergiy; Vasconcelos, Marta W.Life Cycle Assessment (LCA) was conducted to evaluate the environmental impact of cultivating black (Desi type) and white (Kabuli type) chickpeas in Europe under both organic and conventional farming systems. The LCA on a mass basis (1 kg of product) showed that Kabuli chickpea cultivation had a lower global warming potential, water use, and land occupation due to higher yields and more established farming practices for this variety in conventional systems. This study also examined the environmental impact of producing hummus derived from these two raw materials. The LCA on a mass basis (1 kg of product) showed that hummus production generally had a lower environmental impact when using Kabuli chickpeas primarily due to higher yield efficiency and optimised farming practices for the Kabuli type. However, when nutritional LCA is considered, using Nutrient Density as a functional unit (NDU), Desi hummus demonstrates lower environmental impacts in most categories, particularly regarding eutrophication and acidification potential. The study highlights the trade-offs between environmental impacts and nutritional benefits while comparing organic and conventional farming systems, suggesting that incorporating underutilised types such as Desi chickpeas could contribute to a more sustainable and nutritionally diverse food system.
- Correction to: resistance of Pinus pinea to Bursaphelenchus xylophilus explained by the dynamic response of phytohormones, antioxidant activity, and stress-related gene expression (Trees, (2025), 39, 1, (21), 10.1007/s00468-024-02594-7)Publication . Silva, Marta Nunes da; Santos, Carla S.; Solla, Alejandro; Gamir, Jordi; Flors, Victor; Sampedro, Luis; Zas, Rafael; Vasconcelos, Marta W.The corresponding author name has been incorrectly swapped in the original publication. The complete correct name should read as follows. Marta Nunes da Silva In addition, the funding information has been revised. The complete correct funding information is given below. The study was performed within the ‘Characterization of the pinewood nematode/Pinus system: a phytochemical and histopatho-logical approach’ project (PTDC/AGR-CFL/120184/2010), funded by Fundação para a Ciência e a Tecnologia (FCT). Additional financial support came from the Spanish National Research Agency FENOPIN (AGL2012-40151), RESILPINE (RTI2018-094691-B-C33) grants, Intramural-201640I030, OTR07700, IN607A2021/03, and from the FCT’s bilateral action Spain-Portugal PRIAIBPT-2011-1152 (NEMARES), UIDB/50016/2020 R&D Unit, and 2022.01903.CEECIND and 2023.06124.CEECIND programs. The original article has been corrected.
- Development of a healthy lentil-based food productPublication . Geraldo, Rafaela A. F.; Santos, Carla S.; Silva, Marta Nunes da; Pinto, Elisabete; Vasconcelos, Marta W.
- Differential responses of Kabuli and Desi chickpeas (Cicer arietinum) to low water provision and their mineral profilingPublication . Medeiros, Janaína; Silva, Marta Nunes da; Santos, Carla S.; Vasconcelos, Marta W.Legume grains are of great importance for agriculture and the environment due to their ability to symbiotically fix atmospheric nitrogen and provide protein, minerals, vitamins, and other bioactive nutrients (Geraldo et al., 2022). Chickpea (Cicer arietinum) is one of the most consumed legumes worldwide and it has gained even more importance in recent decades. Production levels have increased, their incorporation as intercrops has been promoted, and they have been used in the formulation of novel food products (Saget et al., 2020). Nonetheless, the exploitation of traditional chickpea varieties, such as the Desi type (black coloured chickpea), has been overlooked, and the recovery of under-exploited traditional varieties could contribute to fostering biodiversity, and promoting environmental sustainability and diversifying diets. However, current knowledge on the nutritional profile of commercial and traditional chickpea varieties and their resilience degree to environmental stresses, such as water scarcity, is limited, thus being the focus of this work.