Browsing by Author "Pinto, R."
Now showing 1 - 6 of 6
Results Per Page
Sort Options
- Assessing preschoolers interactive behaviour: a validation study of the “Coding System for Mother–Child Interaction”Publication . Baiao, Rita; Baptista, Joana; Carneiro, A.; Pinto, R.; Toscano, C.; Fearon, P.; Soares, Isabel; Mesquita, A.R.Background: The preschool years are a period of great developmental achievements, which impact critically on a child's interactive skills. Having valid and reliable measures to assess interactive behaviour at this stage is therefore crucial. The aim of this study was to describe the adaptation and validation of the child coding of the Coding System for Mother–Child Interactions and discuss its applications and implications in future research and practice. Methods: Two hundred twenty Portuguese preschoolers and their mothers were videotaped during a structured task. Child and mother interactive behaviours were coded based on the task. Maternal reports on the child's temperament and emotional and behaviour problems were also collected, along with family psychosocial information. Results: Interrater agreement was confirmed. The use of child Cooperation, Enthusiasm, and Negativity as subscales was supported by their correlations across tasks. Moreover, these subscales were correlated with each other, which supports the use of a global child interactive behaviour score. Convergent validity with a measure of emotional and behavioural problems (Child Behaviour Checklist 1 ½–5) was established, as well as divergent validity with a measure of temperament (Children's Behaviour Questionnaire–Short Form). Regarding associations with family variables, child interactive behaviour was only associated with maternal behaviour. Conclusions: Findings suggest that this coding system is a valid and reliable measure for assessing child interactive behaviour in preschool age children. It therefore represents an important alternative to this area of research and practice, with reduced costs and with more flexible training requirements. Attention should be given in future research to expanding this work to clinical populations and different age groups.
- Caracterização hemorreológica, bioquímica e cardiovascular num modelo de doença renal crónica moderada em ratoPublication . Garrido, P.; Costa, E.; Teixeira-Lemos, E.; Parada, B.; Teixeira, M.; Santos, P.; Piloto, N.; Sereno, J.; Alves, R.; Pinto, R.; Rocha-Pereira, P.; Figueiredo, A.; Nunes, S.; Romão, A. M.; Carvalho, L.; Couceiro, P.; Belo, L.; Santos-Silva, A.; Teixeira, F.; Reis, F.Chronic kidney disease (CKD) is a major public health problem throughout the world. The major outcomes include a rapid progression, with development of anaemia and serious complications, namely thromboembol ic and cardiovascular events. The pathophysiological alterations depend on the CKD degree, which will also determine the moment to initiate hemodialysis and recombinant erythropoietin (rhEPO) therapies Thus, the cardio-renal complication might be better prevented or delayed if CKD patients are earlier identified and treated for the associated anaemia, which will depend on a better characterization of moderate stages of CKD. This study aimed to characterization an animal of model of moderate CKD induced by partial (%) nephrectomy, by evaluating hemorheological, biochemical and cardiovascular profiles. Blood samples from control and CKD rats were collected at 0, 3, 9 and 15 weeks in order to evaluate: renal function, hemorheological parameters, iron metabolism, blood lipids, peripheral sympathetic and serotonergic systems, redox state and inflammatory markers. BP, tissues uophism indexes and kidney histomorphology were also assessed. Our data is consistent with a sustained moderate degree of CKD with a quickly compensated modest anaemia, though presenting iron metabolism disturbances. Despite the reasonable degree of functionality of the remnant kidney, as suggested by the anaemia correction and by the kidney hypertrophy, several important cardiovascular modifications were developed. Our model presented hypertension, dyslipidaemia, erythropoietic disturbances, sympathetic activation and oxidative stress. This model might be a good tool to study the cellular/molecularmechanisms underlying moderate stages of CKD and to evaluate the therapeutics efficacy for prevention, treatment/correction of cardiorenal anaemia syndromes and complications in early stages.
- Effect of recombinant human erythropoietin in a rat model of moderate chronic renal failure – focus on inflammation, oxidative Stress and function/renoprotectionPublication . Garrido, P.; Reis, F.; Costa, E.; Almeida, A.; Parada, B.; Teixeira-Lemos, E.; Santos, P.; Alves, R.; Sereno, J.; Pinto, R.; Tavares, C.A.; Figueiredo, A.; Rocha-Pereira, P.; Belo, L.; Santos-Silva, A.; Teixeira, F.Background/Aims: Chronic renal failure (CRF) patients develop anaemia, thus promoting cardiovascular complications, which seems to be favoured by the low kidney erythropoietin (EPO) production. The renal insufficiency degree might determine the moment to start recombinant human EPO (rhEPO) therapy. It has been attributed important non-hematopoietic effects to rhEPO, which might underlie cardio and renoprotection. This work aimed to evaluate the effect of rhEPO in a rat model of moderate CRF, focusing on inflammation, oxidative stress and function/renoprotection. Methods: Four groups (n=7) of male Wistar rats were evaluated during a 15 week follow-up period: control (without treatment); rhEPO (50 IU/Kg/wk Recormon®); CRF and CRF+rhEPO. Blood samples were collected at the beginning and 3, 9 and 12 weeks after 3/4 nephrectomy, in order to evaluate: renal function, haematological parameters, iron metabolism and serum proliferative (TGF-B1), inflammatory (TNF-a, CRP, IL-2 and IL-1B) and redox status (MDA, TAS and 3-NT) markers. Kidney gene expression of Il2, Vegf, Nos2 and Nos3 were assessed by real-time PCR. Blood pressure, heart rate and tissues trophy indexes were also estimated. Results: Our data are consistent with a sustained moderate degree of CRF with development of moderate and corrected anaemia and hypertension. The remnant kidney showed a proliferative profile, with increased mass (hypertrophism), upregulated tissue Vegf gene expression, accompanied by increased levels of serum TGF-B1. Serum 3-NT was augmented, suggesting oxidative stress, which was accompanied by a trend to higher kidney Nos gene expression of both isoforms. rhEPO treatment was able to partially attenuate renal function markers, totally correct anaemia, also demonstrating a proliferative and antioxidant action, suggesting renoprotection. Conclusion: This study suggests that rhEPO therapy might be recommended in moderate CRF stages in order to efficiently correct not only the anaemia but also the underlying deleterious mechanisms, due to a proliferative and antioxidant action on the remnant kidney.
- Erythropoietin doping as cause of sudden death in athletes: an experimental studyPublication . Piloto, N.; Teixeira, H.M.; Teixeira-Lemos, E.; Parada, B.; Garrido, P.; Sereno, J.; F. Pinto, A.F.; Costa, Elísio; Belo, L.; Santos-Silva, A.; Pinto, R.; Couceiro, P.; Neto, P.; Xavier, F.; Carvalho, L.; Teixeira, F.; Reis, F.Aims: To evaluate the cardiovascular (CV) effects of rhEPO treatment in rats under chronic aerobic exercise and to assess the probable cause of sudden death in one rat. Protocol: Male Wistar rats: control - sedentary; rhEPO - 50 IU/Kg/3xwk; swimming (EX) -1 hr, 3x/wk; EX+EPO. Hematology, catecholamines and serotonin, redox status and inflammation, were assessed. One rat of EX+EPO group suffered a sudden death episode. Results: rhEPO treatment in trained rats promoted several markers of increased CV risk. The sudden death rat tissues presented: lungs without signs of drowning; brain with vascular congestion; LV hypertrpphy and deregulations of cardiac fibers, together with a "cardiac liver", suggesting the hypothesis of heart failure as cause of death. Conclusion: The sudden death of a EX+EPO rat, due to a cardiac episode, together with the increased CV risk profile, strongly suggest a high life risk associated to the continuous rhEPO doping. The anatomo-pathological studies were determinant to establish the cause of death.
- Recombinant human erythropoietin therapy has beneffical cardio-renal effects on moderate stages of chronic renal failure in the ratPublication . Garrido, P.; Reis, F.; Costa, Elísio; Parada, B.; Piloto, N.; Sereno, J.; Teixeira, A.; Pinto, R.; Figueiredo, A.; Alves, R.; Rocha-Pereira, P.; Belo, L.; Santos-Silva, A.; Teixeira, F.This study aimed to assess the cardio-renal effects of rhEPO therapy on an animal model of moderate chronic renal failure (CRF). Four groups (n =7) of male rat were evaluated during a 12-week follow up period: control; rhEPO: 50 IU/Kg/wk; CRF: two-stage 3/, nephrectomy; CRF+ rhEPO (start after the 3'd wk of surgery). Renal function, haematology and serum inflammation and redox status were assessed. rhEPO treatment was able to partially attenuate renal function markers, totally correct anaemia, also showing a proliferative and antioxidant action, due to increased serum TGF-13I and decreased 3-NT. In conclusion, rhEPO therapy might be recommended in moderate CRF stages in order to efficiently correct not only the underlying anaemia but also the deleterious cardio-renal effects, due to a proliferative and antioxidant renoprotective action.
- The effect of the addition of fresh and dried starter cultures on microbiological and chemical parameters of a smoked sausage “Alheira”Publication . Barros, D.; Velho, M. Vaz; Pinto, R.; Pinheiro, R.; Fonseca, S.; Macieira, A.; Albano, Helena; Morais, A. M.; Teixeira, P.