Caracterização hemorreológica, bioquímica e cardiovascular num modelo de doença renal crónica moderada em rato

Chronic kidney disease (CKD) is a major public health problem throughout the world. The major outcomes include a rapid progression, with development of anaemia and serious complications, namely thromboembol ic and cardiovascular events. The pathophysiological alterations depend on the CKD degree, which will also determine the moment to initiate hemodialysis and recombinant erythropoietin (rhEPO) therapies Thus, the cardio-renal complication might be better prevented or delayed if CKD patients are earlier identified and treated for the associated anaemia, which will depend on a better characterization of moderate stages of CKD. This study aimed to characterization an animal of model of moderate CKD induced by partial (%) nephrectomy, by evaluating hemorheological, biochemical and cardiovascular profiles. Blood samples from control and CKD rats were collected at 0, 3, 9 and 15 weeks in order to evaluate: renal function, hemorheological parameters, iron metabolism, blood lipids, peripheral sympathetic and serotonergic systems, redox state and inflammatory markers. BP, tissues uophism indexes and kidney histomorphology were also assessed. Our data is consistent with a sustained moderate degree of CKD with a quickly compensated modest anaemia, though presenting iron metabolism disturbances. Despite the reasonable degree of functionality of the remnant kidney, as suggested by the anaemia correction and by the kidney hypertrophy, several important cardiovascular modifications were developed. Our model presented hypertension, dyslipidaemia, erythropoietic disturbances, sympathetic activation and oxidative stress. This model might be a good tool to study the cellular/molecularmechanisms underlying moderate stages of CKD and to evaluate the therapeutics efficacy for prevention, treatment/correction of cardiorenal anaemia syndromes and complications in early stages.

Keywords

Moderate chronic kidney disease Partial nephrectomy Rat model Hemorheological data, Renal function Iron metabolism Cardiovascular profile

URI

http://hdl.handle.net/10400.14/4428

Citation

GARRIDO, P. ... [et al.] - Caracterização hemorreológica, bioquímica e cardiovascular num modelo de doença renal crónica moderada em rato. Boletim Sociedade da Portuguesa de Hemorreologia e Microcirculação. ISSN 0872-4938. Vol 25, n.º2 (2010), p. 9-22