Percorrer por autor "Figueiredo, A."
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- Acção de sensibilização na Escola Secundária Alves Martins, ViseuPublication . Gonçalves, J. C.; Bogalheiro, M.; Melo, J.; Alves, M.; Figueiredo, A.; Veiga, N.
- Avaliação da saúde oral numa população ruralPublication . Barata, C.; Veiga, N.; Figueiredo, A.; Santos, L.
- Caracterização hemorreológica, bioquímica e cardiovascular num modelo de doença renal crónica moderada em ratoPublication . Garrido, P.; Costa, E.; Teixeira-Lemos, E.; Parada, B.; Teixeira, M.; Santos, P.; Piloto, N.; Sereno, J.; Alves, R.; Pinto, R.; Rocha-Pereira, P.; Figueiredo, A.; Nunes, S.; Romão, A. M.; Carvalho, L.; Couceiro, P.; Belo, L.; Santos-Silva, A.; Teixeira, F.; Reis, F.Chronic kidney disease (CKD) is a major public health problem throughout the world. The major outcomes include a rapid progression, with development of anaemia and serious complications, namely thromboembol ic and cardiovascular events. The pathophysiological alterations depend on the CKD degree, which will also determine the moment to initiate hemodialysis and recombinant erythropoietin (rhEPO) therapies Thus, the cardio-renal complication might be better prevented or delayed if CKD patients are earlier identified and treated for the associated anaemia, which will depend on a better characterization of moderate stages of CKD. This study aimed to characterization an animal of model of moderate CKD induced by partial (%) nephrectomy, by evaluating hemorheological, biochemical and cardiovascular profiles. Blood samples from control and CKD rats were collected at 0, 3, 9 and 15 weeks in order to evaluate: renal function, hemorheological parameters, iron metabolism, blood lipids, peripheral sympathetic and serotonergic systems, redox state and inflammatory markers. BP, tissues uophism indexes and kidney histomorphology were also assessed. Our data is consistent with a sustained moderate degree of CKD with a quickly compensated modest anaemia, though presenting iron metabolism disturbances. Despite the reasonable degree of functionality of the remnant kidney, as suggested by the anaemia correction and by the kidney hypertrophy, several important cardiovascular modifications were developed. Our model presented hypertension, dyslipidaemia, erythropoietic disturbances, sympathetic activation and oxidative stress. This model might be a good tool to study the cellular/molecularmechanisms underlying moderate stages of CKD and to evaluate the therapeutics efficacy for prevention, treatment/correction of cardiorenal anaemia syndromes and complications in early stages.
- Characterization of L. monocytogenes in a portuguese cheese production plantPublication . Almeida, G.; Carneiro, L.; Figueiredo, A.; Hogg, T.; Teixeira, P.
- Effect of recombinant human erythropoietin in a rat model of moderate chronic renal failure – focus on inflammation, oxidative Stress and function/renoprotectionPublication . Garrido, P.; Reis, F.; Costa, E.; Almeida, A.; Parada, B.; Teixeira-Lemos, E.; Santos, P.; Alves, R.; Sereno, J.; Pinto, R.; Tavares, C.A.; Figueiredo, A.; Rocha-Pereira, P.; Belo, L.; Santos-Silva, A.; Teixeira, F.Background/Aims: Chronic renal failure (CRF) patients develop anaemia, thus promoting cardiovascular complications, which seems to be favoured by the low kidney erythropoietin (EPO) production. The renal insufficiency degree might determine the moment to start recombinant human EPO (rhEPO) therapy. It has been attributed important non-hematopoietic effects to rhEPO, which might underlie cardio and renoprotection. This work aimed to evaluate the effect of rhEPO in a rat model of moderate CRF, focusing on inflammation, oxidative stress and function/renoprotection. Methods: Four groups (n=7) of male Wistar rats were evaluated during a 15 week follow-up period: control (without treatment); rhEPO (50 IU/Kg/wk Recormon®); CRF and CRF+rhEPO. Blood samples were collected at the beginning and 3, 9 and 12 weeks after 3/4 nephrectomy, in order to evaluate: renal function, haematological parameters, iron metabolism and serum proliferative (TGF-B1), inflammatory (TNF-a, CRP, IL-2 and IL-1B) and redox status (MDA, TAS and 3-NT) markers. Kidney gene expression of Il2, Vegf, Nos2 and Nos3 were assessed by real-time PCR. Blood pressure, heart rate and tissues trophy indexes were also estimated. Results: Our data are consistent with a sustained moderate degree of CRF with development of moderate and corrected anaemia and hypertension. The remnant kidney showed a proliferative profile, with increased mass (hypertrophism), upregulated tissue Vegf gene expression, accompanied by increased levels of serum TGF-B1. Serum 3-NT was augmented, suggesting oxidative stress, which was accompanied by a trend to higher kidney Nos gene expression of both isoforms. rhEPO treatment was able to partially attenuate renal function markers, totally correct anaemia, also demonstrating a proliferative and antioxidant action, suggesting renoprotection. Conclusion: This study suggests that rhEPO therapy might be recommended in moderate CRF stages in order to efficiently correct not only the anaemia but also the underlying deleterious mechanisms, due to a proliferative and antioxidant action on the remnant kidney.
- Hypertension induced by immunosuppressive drugs: a comparative analysis between sirolimus and cyclosporinePublication . Reis, F.; Parada, B.; Lemos, E. Teixeira de; Garrido, P.; Dias, A.; Piloto, N.; Baptista, S.; Sereno, J.; Eufrásio, P.; Costa, Elísio; Rocha-Pereira, P.; Santos-Silva, A.; Figueiredo, A.; Mota, A.; Teixeira, F.The purpose of this study was to compare the effects of sirolimus (SRL) vs cyclosporine (CsA) concerning the cardiovascular mechanisms hypothetically contributing to hypertension development. Three rat groups were studied: control (vehicle), CsA (5 mg/kg/d), and SRL (1 mg/kg/d). The following parameters were evaluated after 7 weeks of treatment: blood pressured (BP) and heart rate (HR; tail cuff), lipid profile, hematology, plasma and platelet 5-HT and catecholamines (HPLC-ECD), and oxidative equilibrium (serum malondialdehyde [MDA] and total antioxidant status [TAS]). Systolic (SBP) and diastolic blood pressure (DBP) values were higher (P < .001) in both the CsA (146.2 +/- 4.5 and 124.9 +/- 4.5 mm Hg) and SRL (148.9 +/- 4.8 and 126.4 +/- 6.0 mm Hg) groups vs the controls (115.9 +/- 3.3 and 99.1 +/- 2.0 mm Hg). However, HR values were elevated in CsA but not SRL animals. The dyslipidemic pattern of CsA was even more enhanced in the SRL group, with significantly higher low-density lipoprotein cholesterol (LDL-c) and triglyceride (TG) levels vs CsA (P<.05); red blood cells, hematocrit, hemoglobin concentration, mean platelet volume, and platelet distribution width were significantly (P<.05) higher in the SRL vs CsA group. The pro-oxidative profile (increased MDA/TAS) in the CsA group was not reproduced in the SRL cohort. While plasma and platelet 5-HT were elevated in SRL rats, catecholamine content was higher in CsA animals. In conclusion, this study demonstrated that CsA and SRL produce identical hypertensive effects. However, while CsA promotes oxidative stress and sympathetic activation, SRL mainly interferes with lipid profile and hematological parameters. Thus, the hypertensive effects of CsA, a calcineurin inhibitor, and of SRL, an mTOR inhibitor, are associated with impairment of distinct cardiovascular pathways.
- Rapid correction of anterior crossbite using a bonded compomer slopePublication . Alves, J.; Seabra, M.; Figueiredo, A.An anterior crossbite can hardly be corrected spontaneously. When diagnosed in a mixed dentition or in an early permanent dentition, an interceptive orthodontic treatment becomes imperative. The objective of this paper is to report a rapid, cheap and effective interceptive orthodontic treatment of an anterior crossbite in a pediatric patient in mixed dentition. A male patient aged 10 years old reported an important aesthetic embarrassment of his smile as well as, functional problems. An anterior crossbite of tooth 2.1 was diagnosed, hiding almost the entire clinical crown. Dental crossbite was corrected by bonding a compomer slope to the incisal edge of the mandibular incisor with an angle of about 45° to the longitudinal axis of the tooth. Correction was achieved within 3 weeks of treatment, with no damage to the tooth or periodontal tissue. As a conclusion, a simple bonded compomer slope, done in just one clinical appointment can solve both aesthetic and functional problems in a very simple way. It is cheap, cost-effective and very easy for patients to accept. The technique is so elementary, that not only pediatric dentistry experts but every general dentist is able to perform it. If we are able to treat an anterior crossbite at the exact moment of the diagnosis, then, the need for long term orthodontic treatment can reduced, thereby, giving the kids a chance for growing up in a more healthy way.
- Recombinant human erythropoietin therapy has beneffical cardio-renal effects on moderate stages of chronic renal failure in the ratPublication . Garrido, P.; Reis, F.; Costa, Elísio; Parada, B.; Piloto, N.; Sereno, J.; Teixeira, A.; Pinto, R.; Figueiredo, A.; Alves, R.; Rocha-Pereira, P.; Belo, L.; Santos-Silva, A.; Teixeira, F.This study aimed to assess the cardio-renal effects of rhEPO therapy on an animal model of moderate chronic renal failure (CRF). Four groups (n =7) of male rat were evaluated during a 12-week follow up period: control; rhEPO: 50 IU/Kg/wk; CRF: two-stage 3/, nephrectomy; CRF+ rhEPO (start after the 3'd wk of surgery). Renal function, haematology and serum inflammation and redox status were assessed. rhEPO treatment was able to partially attenuate renal function markers, totally correct anaemia, also showing a proliferative and antioxidant action, due to increased serum TGF-13I and decreased 3-NT. In conclusion, rhEPO therapy might be recommended in moderate CRF stages in order to efficiently correct not only the underlying anaemia but also the deleterious cardio-renal effects, due to a proliferative and antioxidant renoprotective action.
- Recommendations for the implementation of a national lung cancer screening program in Portugal - a consensus statementPublication . Fernandes, M. G. O.; Dias, M.; Santos, R.; Ravara, S.; Fernandes, P.; Firmino-Machado, J.; Antunes, J. P.; Fernandes, O.; Madureira, A.; Hespanhol, V.; Rodrigues, C.; Vicente, C. A.; Alves, S.; Mendes, G.; Ilgenfritz, R.; Pinto, B. S.; Alves, J.; Saraiva, I.; Bárbara, C.; Cipriano, M. A.; Figueiredo, A.; Uva, M. S.; Jacinto, N.; Curvo-Semedo, L.; Morais, A.Lung cancer (LC) is a leading cause of cancer-related mortality worldwide. Lung Cancer Screening (LCS) programs that use low-dose computed tomography (LDCT) have been shown to reduce LC mortality by up to 25 % and are considered cost-effective. The European Health Union has encouraged its Member States to explore the feasibility of LCS implementation in their respective countries. The task force conducted a comprehensive literature review and engaged in extensive discussions to provide recommendations. These recommendations encompass the essential components required to initiate pilot LCS programs following the guidelines established by the World Health Organization. They were tailored to align with the specific context of the Portuguese healthcare system. The document addresses critical aspects, including the eligible population, methods for issuing invitations, radiological prerequisites, procedures for reporting results, referral processes, diagnostic strategies, program implementation, and ongoing monitoring. Furthermore, the task force emphasized that pairing LCS with evidence-based smoking cessation should be the standard of care for a high-quality screening program. This document also identifies areas for further research. These recommendations aim to guarantee that the implementation of a Portuguese LCS program ensures high-quality standards, consistency, and uniformity across centres.
- Serotypes of Listeria monocytogenes Isolated from Human Cases of Listeriosis in PortugalPublication . Almeida, G.; Magalhães, R.; Figueiredo, A.; Carneiro, L.; Teixeira, P.; Hogg, T.