Veritati

Institutional Repository of the Universidade Católica Portuguesa

The Institutional Repository (Veritati) is a reference platform for those who want to access scientific production, as well as master's dissertations and doctoral theses, developed within the scope of the teaching and research activities of the Universidade Católica Portuguesa (UCP).

As part of the RCAAP project (Repositórios Científicos de Acesso Aberto de Portugal), it aims to preserve and disseminate scientific production, thus increasing its visibility and impact.

The Institutional Repository is also integrated into the UCP science management ecosystem, which includes, namely, the Ciência-UCP platform and the Scientific Journals of Universidade Católica Portuguesa portal

Universidade Católica Portuguesa Resources

Email repositorio@ucp.pt

Recent Submissions

European seabass response to air exposure: impact of dietary inclusion of bioactive blood hydrolysates

Publication . Resende, Daniela; Pereira, Ricardo; Velasco, Cristina; Domínguez, David; Pereira, Miguel; Pereira, Carlos; Marques, Bianca; Rocha, Cristina; Lima, Rui Costa; Pintado, Manuela; Valente, Luísa M. P.

In aquaculture farms, fish are subjected to stress situations, which induce oxidative stress through an imbalance between the generation of reactive oxygen species (ROS) and the scavenging activity of antioxidants. Diet supplementation with functional ingredients to modulate oxidative stress after air exposure was investigated, using bioactive peptides from swine blood hydrolysates (BH), within a circular economy context. Three swine BH were obtained by autohydrolysis (AH) or enzymatically. The enzymatically produced BH were further submitted to a micro- (MF) or nanofiltration (NF). Five isolipidic and isoproteic diets for European seabass were developed: a fishmeal (FM) based diet (positive control, PC), a commercial-based diet where 50% of FM was replaced by vegetable proteins (negative control, NC) and three diets where 3% of each BH was added to the NC. Diets were assigned to triplicate groups of 71 European seabass juveniles (initial weight 12.3 ± 1.4 g), and fed to apparent satiation in a recirculating saltwater system. After 12 weeks, 9 fish per treatment were either immediately sampled or air-exposed for 1 minute and let to recover for 6 hours prior to sampling, in which plasma and liver were collected. Plasmatic cortisol and lactate were elevated for all diets after the air exposure, without differences among diets. Glucose levels were unaffected by diet or stress. Regarding liver oxidative stress markers, lipid peroxidation (LPO) tended to increase after air exposure. Considering the non-stressed fish, MF diet led to the lowest LPO, being significantly lower than the AH, and similar to the remaining diets. Protein oxidation decreased in stressed fish, but did not differ among diets. Liver catalase was significantly lower in NC, NF and AH groups compared to PC and increased after stress. Superoxide dismutase activity was lower for AH and PC diets than for the MF, and it was reduced in stressed fish. The stress response triggered by air exposure involved increased cortisol levels, followed by an increment in plasma lactate. However, none of the BH improved plasma stress response. The stress challenge increased liver LPO through ROS accumulation under oxidative stress. Carbonyls decreased post-stress, likely due to a feedback interaction with the LPO radicals, which reduced protein oxidation. The NF, composed of smaller peptides than the other BH, may modulate European seabass antioxidant defences by lowering catalase levels without increasing LPO, suggesting it could provide exogenous antioxidants to counteract ROS-induced oxidative stress.

2021-09Conference object

Open access

Effect of the dietary inclusion of blood hydrolysates on European seabass response to stress

Publication . Resende, Daniela; Pereira, Ricardo; Velasco, Cristina; Domínguez, David; Pereira, Miguel; Pereira, Carlos; Marques, Bianca; Rocha, Cristina; Pintado, Manuela; Valente, Luísa M. P.

2021-10Conference object

Open access

Avaliação das dimensões dos dentes ântero-superiores numa amostra populacional de Viseu com recurso a imagens de scanner intraoral

Publication . Nikulina, Miroslava Stefanova; Salgado, Helena Cristina Morais Coelho Teixeira; Araújo, Filipe Miguel Soares Framegas de

INTRODUÇÃO: A Medicina Dentária tem vindo a integrar, de forma crescente, componentes estéticos na sua prática e aplicação clínica, refletindo a valorização do sorriso na imagem pessoal. A Reabilitação Oral deixou de ter como único objetivo a restituição da função, passando a exigir resultados estéticos que atendam às expectativas dos pacientes. Neste contexto, os dentes ântero-superiores assumem um papel dominante no sorriso, estando intrinsecamente ligados à sua harmonia. O principal objetivo deste trabalho é analisar as caraterísticas antropométricas e a existência de Proporção Dourada entre os dentes ântero-superiores na amostra em estudo. MATERIAIS E MÉTODOS: Foram analisados modelos digitais da maxila, obtidos através do scanner intraoral de 82 indivíduos. Após importação no programa Blender, foram realizadas as medições das dimensões dentárias, nomeadamente a largura e a altura das coroas dos 6 dentes ântero-superiores. Os dados obtidos foram sujeitos a uma análise estatística descritiva e inferencial, com um limiar de significância de p<0,05. RESULTADOS: A análise antropométrica demonstrou diferenças estatisticamente significativas em todas as variáveis das dimensões dentárias entre os sexos biológicos (p<0,05). Foram igualmente demonstradas assimetrias dimensionais entre dentes contralaterais, confirmadas pelos testes T de amostras emparelhadas. Os valores da proporção altura-largura das coroas dentárias foram superiores ao Golden Standard, que preconiza valores entre 75% e 80%. Foram relatadas diferenças estatisticamente significativas entre o valor real e o valor esperado das larguras aparentes das coroas dentárias em todos os dentes analisados, o que demonstra a ausência de Proporção Dourada. CONCLUSÕES: A análise antropométrica permitiu confirmar a existência de dimorfismo sexual e de assimetrias entre os dentes contralaterais. Apesar da estética dentária estar relacionada com padrões estéticos pré-definidos, como são exemplo o Golden Standard e a Proporção Dourada, estes não foram verificados na amostra em estudo.

2025-07-04Master thesis

Embargoed access

Overview of generative processes in the work of Brian Eno

Publication . Gradim, Rita; Pestana, Pedro Duarte

Brian Eno is a famous a musician, producer and artist known for his endeavors in rock music who has devoted the latter part of his career to ambient and generative work. There are a few publications regarding his work (Albiez & Pattie, 2016; Sheppard, 2009; Tamm, 1988), but they do not focus exclusively on his generative processes. The only paper that details Eno’s generative music only addresses the concepts of generative music and describes Eno’s apps (Marshall & Loydell, 2017). The paper focuses more on his apps and it does not present a detailed correlation between his background/ influences to his work. This paper bridges that specific gap, by detailing Eno’s school background, his influences, generative processes and how he moved on from traditional to more technological approaches.

2021-10-12Conference paper not in proceedings

Open access

Metagenomic assessment of the microbial diversity of swine carcasses

Publication . Carvalho, Marta; Moura-Alves, Márcio; Saraiva, Cristina; Esteves, Alexandra; Ribeiro, Deise; Leonardo, Inês Carvalho; Gaspar, Frédéric Bustos; Crespo, Maria Teresa Barreto; Teixeira, Paula

Contamination, cross-contamination, or carcass recontamination can mainly occur along the slaughtering processes due to contact between animals during their transportation, steps of slaughter carcass preparation, slaughterhouse environment, clothing, and poor slaughter and hygiene practices [1]. Evaluating the microbiological profile is important to verify hygiene conditions during the slaughter processes and ensure the food safety of the final product [2]. The purpose of this study was to characterize the microbial community of swine carcasses and environmental samples - knives and drains - using a metagenomic approach. Gauze swabs were used to collect samples from drains and from five animals before and after evisceration, and water from the knives sterilizer was collected in sterile tubes before and after eviscerations. Samples were prepared for Illumina Sequencing by 16S rRNA gene amplification of the bacterial community. All 16S rRNA data were analysed with Kraken v1 using the pre-built MiniKraken 8Gb database (https://ccb.jhu.edu/software/kraken/) with default parameters. Twenty-five genera with abundance above 1% were found. The most abundant genus on carcasses was Anoxybacillus (52% of the total microbiome) and on environmental samples Oenococcus (15%). Enterobacteriaceae were detected in 13 collected points (prevalence ranging from 0.001% to 0.02%), with different genera found: Citrobacter (18.0%), Klebsiella (14.0%), Erwinia (12.1%), Enterobacter (9.99%), Trabulsiella (5.99%), Providencia (4.0%), Serratia (4.0%), Salmonella (4.0%) and Morganella (1.99%). It is important to highlight that Salmonella spp. was found in only two samples, one from the carcass and the other from the drain sampled on the same day. Other genera of interest in food microbiology were also detected, namely, Pseudomonas, Lactobacillus, Campylobacter, Clostridium, Bacillus, Staphylococcus, and Lactococcus. Such diversity suggests possible interventions to extend shelf life hampered by the microbial consortia with pork products. Otherwise, it may be useful for developing microbial traceability to a slaughterhouse.

2021-11-18Conference object

Open access