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Authors
Advisor(s)
Abstract(s)
A cárie dentária é uma doença crónica de etiologia multi-fatorial, causada pela
complexa interação entre os ácidos produzidos pela placa bacteriana, os hidratos de
carbono fermentáveis provenientes da dieta e fatores do hospedeiro que incluem a saliva.
Dada a elevada prevalência da doença a nível mundial e uma base cientifica que suporta a
existência de fatores de suscetibilidade individual para o desenvolvimento da mesma, urge
a necessidade de desenvolver meios de identificação precoce de suscetibilidade para que
medidas preventivas eficazes possam ser tomadas. Assim, com este estudo objetivou-se
verificar a relação existente entre a presença de determinadas proteínas salivares e a
presença de cárie nos indivíduos analisados, com possível identificação de potenciais
marcadores de suscetibilidade à doença.
A catalogação das proteínas salivares em pacientes com cárie dentária, permitiu
adicionar 28 proteínas à base de dados do OralCard (entre as quais 4 microbianas e 24
humanas). A caracterização funcional do OralOma de cárie permitiu identificar alterações
estatisticamente significativas (p≤0,05) em determinados processos biológicos e funções
moleculares das proteínas salivares de cárie dentária, nomeadamente no que respeita à ação
antioxidante da saliva, especificamente por ação do sistema da peroxidase salivar. A análise
interatómica realizada permitiu destacar 4 proteínas (transcobalamin-1, peptidylprolyl cistransisomeraseB,
small proline-rich protein 3 e histone 4) com elevados graus de interação
com o OralOma total e com potencial influência no desenvolvimento de lesões de cárie
dentária.
Verificou-se uma carência de estudos experimentais dirigidos para o proteoma
microbiano em cárie dentária, no entanto a identificação dos seus fatores de virulência pode
constituir um passo na análise da suscetibilidade de um indivíduo ao desenvolvimento da
doença. A análise do proteoma oral realizada permitiu compreender alguns aspetos dos
mecanismos moleculares envolvidos no processo de cárie dentária, permitindo estabelecer
potenciais biomarcadores de suscetibilidade à doença, a serem avaliados em estudos
experimentais no futuro.
Dental carie is a chronic disease of multifactorial etiology caused by a complex interaction between the biofilm produced acids, fermentable carbohydrates from the diet and several host factors including the saliva composition. Due to the worldwide high prevalence of the disease and a large scientific base that supports the existence of individual susceptibility factors for its development, it urges the need to develop early susceptibility assessment methods in a way that preventive measures can be taken urges. Therefore, this study aimes at verifying the relationship between the presence of certain salivary proteins and the presence of dental caries in the analyzed individuals with the identification of potential biomarkers of susceptibility to disease. The listing of patients with salivary proteins in dental caries allowed to add 28 proteins to OralCard database (including 4 microbial and 24 human proteins). Functional characterization of caries OralOme allowed the identification of statistically significant changes (p≤0,05) in certain biological processes and molecular functions of salivary proteins involved in tooth decay, particularly with regard to saliva antioxidant activity by specific action of salivary peroxidase system. The inter-atomic analysis has highlighted 4 proteins (Transcobalamin-1, Peptidylprolyl cis-transisomeraseB, Small proline-rich protein 3 histone 4) with high levels of interaction with the total OralOme and potential influence in dental caries development and progress. A lack of experimental studies directed to the microbial proteome of dental caries was noted; however the identification of virulence factors may be a step to the analysis of individual susceptibility to disease development. The analysis of the oral proteome allowed the understanding of some molecular mechanisms involved in dental caries process, enabling the establishment of potential biomarkers for disease susceptibility, to be evaluated in future experimental studies.
Dental carie is a chronic disease of multifactorial etiology caused by a complex interaction between the biofilm produced acids, fermentable carbohydrates from the diet and several host factors including the saliva composition. Due to the worldwide high prevalence of the disease and a large scientific base that supports the existence of individual susceptibility factors for its development, it urges the need to develop early susceptibility assessment methods in a way that preventive measures can be taken urges. Therefore, this study aimes at verifying the relationship between the presence of certain salivary proteins and the presence of dental caries in the analyzed individuals with the identification of potential biomarkers of susceptibility to disease. The listing of patients with salivary proteins in dental caries allowed to add 28 proteins to OralCard database (including 4 microbial and 24 human proteins). Functional characterization of caries OralOme allowed the identification of statistically significant changes (p≤0,05) in certain biological processes and molecular functions of salivary proteins involved in tooth decay, particularly with regard to saliva antioxidant activity by specific action of salivary peroxidase system. The inter-atomic analysis has highlighted 4 proteins (Transcobalamin-1, Peptidylprolyl cis-transisomeraseB, Small proline-rich protein 3 histone 4) with high levels of interaction with the total OralOme and potential influence in dental caries development and progress. A lack of experimental studies directed to the microbial proteome of dental caries was noted; however the identification of virulence factors may be a step to the analysis of individual susceptibility to disease development. The analysis of the oral proteome allowed the understanding of some molecular mechanisms involved in dental caries process, enabling the establishment of potential biomarkers for disease susceptibility, to be evaluated in future experimental studies.
Description
Keywords
Cárie dentária Suscetibilidade à cárie Proteínas salivares Biomarcadores Proteómica Dental caries Dental caries susceptibility Salivary proteins Biomarkers proteomics