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Sumário: A Gengivite é uma condição inflamatória reversível do periodonto causada
por uma disbiose no biofilme bacteriano que estimula mecanismos imunológicos do
hospedeiro. A inflamação é fisiológica nos estados inicial e precoce, mas a interação
dos fatores de virulência bacterianos com as células imunológicas convertem-na
numa condição patológica irreversível, a Periodontite. A quantificação de
marcadores do biofluido salivar de pacientes com Gengivite poderá elucidar os
mecanismos que levam a esta alteração. As quimiocinas CCL3 e CCL13 são
moléculas mediadoras da resposta imune, pois recrutam células de defesa no
periodonto, e estão propostas enquanto biomarcadores de risco preditivos da
doença periodontal.
Objetivo: Quantificar as CCL3 e CCL13 in vivo em pacientes com Gengivite e
pacientes saudáveis. Avaliar se estes biomarcadores identificam a presença de
inflamação periodontal, a sua extensão, o potencial de progressão da doença e se
permitem o diagnóstico salivar diferencial da Doença Periodontal em pacientes com
Gengivite.
Material e Métodos: 48 indivíduos foram sujeitos a exame clínico intra-oral, e
consoante os parâmetros clínicos Periodontais foram agrupados em dois grupos (S
e G). Foram sujeitos ao preenchimento de um questionário sobre historial médico,
hábitos alimentares e qualidade de vida para melhor caracterização dos grupos de
estudo. Foram posteriormente submetidos a recolha de amostras de saliva para
quantificação das CCL3 e CCL13 por tecnologia Multiplex.
Resultados: As CCL3 e CCL13 foram detectadas na saliva em baixas
concentrações, e os valores no grupo G foram maiores do que no grupo S, não
tendo sido as diferenças estatisticamente significativas (p>0,05). Ao correlacionar os
valores de BOP e IP dos grupos S e G com a concentração das CCL, os valores
encontrados não foram estatisticamente diferentes (p>0,05).
Conclusões: Os resultados obtidos não permitem propor a CCL3 e CCL13 como
bons biomarcadores para avaliar o potencial de progressão da Doença Periodontal
em pacientes com Gengivite.
Summary: Gingivitis is a reversible inflammatory condition of the periodontium caused by a dysbiosis in bacterial biofilms, which stimulates the host immune mechanisms. The inflammatory condition is physiological in the initial and premature states, but the interaction between bacterial virulence factors and immune cells convert it in an irreversible pathological condition, Periodontitis. The quantification of salivary biofluid markers of patients with Gingivitis can elucidate the mechanisms that lead to this change. The chemokine CCL3 and CCL13 are mediator molecules of the immune response, as they recruit the periodontium defense cells, and are proposed as biomarkers of predictive risk for Periodontal Disease. Objective: Quantifying CCL3 and CCL13 in vivo in patients with Gingivitis and in healthy patients. Evaluate if those biomarkers identify the presence of periodontal inflammation, its extension, the potential of disease progression and also if they allow salivary differential diagnosis of Periodontal Disease in patients with Gingivitis. Material and Methods: 48 individuals were subjected to an intra-oral clinical exam, and according with periodontal clinical parameters, were grouped in two groups (S and G). They were subjected to a questionnaire about their medical history, eating habits and life quality, to achieve a better characterization of the study groups. They were subsequently submitted to collection of saliva samples for quantification of CCL3 and CCL 13,using Multiplex technology. Results: CCL3 and CCL13 were detected in low concentrations on saliva, and the values registered in the G group were higher than the values registered in the S group, without statistically significant difference (p>0,05). By correlating the values of BOP and PI of the S and G groups, with the CCL concentration, the values identified were not statistically different (p>0,05). Conclusions: The results do not allow the suggestion of CCL3 and CCL13 as biomarkers to evaluate the progression potential of Periodontal Disease in patients with Gingivitis
Summary: Gingivitis is a reversible inflammatory condition of the periodontium caused by a dysbiosis in bacterial biofilms, which stimulates the host immune mechanisms. The inflammatory condition is physiological in the initial and premature states, but the interaction between bacterial virulence factors and immune cells convert it in an irreversible pathological condition, Periodontitis. The quantification of salivary biofluid markers of patients with Gingivitis can elucidate the mechanisms that lead to this change. The chemokine CCL3 and CCL13 are mediator molecules of the immune response, as they recruit the periodontium defense cells, and are proposed as biomarkers of predictive risk for Periodontal Disease. Objective: Quantifying CCL3 and CCL13 in vivo in patients with Gingivitis and in healthy patients. Evaluate if those biomarkers identify the presence of periodontal inflammation, its extension, the potential of disease progression and also if they allow salivary differential diagnosis of Periodontal Disease in patients with Gingivitis. Material and Methods: 48 individuals were subjected to an intra-oral clinical exam, and according with periodontal clinical parameters, were grouped in two groups (S and G). They were subjected to a questionnaire about their medical history, eating habits and life quality, to achieve a better characterization of the study groups. They were subsequently submitted to collection of saliva samples for quantification of CCL3 and CCL 13,using Multiplex technology. Results: CCL3 and CCL13 were detected in low concentrations on saliva, and the values registered in the G group were higher than the values registered in the S group, without statistically significant difference (p>0,05). By correlating the values of BOP and PI of the S and G groups, with the CCL concentration, the values identified were not statistically different (p>0,05). Conclusions: The results do not allow the suggestion of CCL3 and CCL13 as biomarkers to evaluate the progression potential of Periodontal Disease in patients with Gingivitis
Descrição
Palavras-chave
Doença periodontal Gengivite Resposta inflamatória Quimiocinas Saliva Biomarcadores salivares Diagnóstico salivar Multiplex Periodontal disease Gingivitis Inflammatory response Chemokine Salivary biomarkers Salivary diagnosis Multiplex