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Abstract(s)
A doença periodontal constitui uma doença de elevada prevalência no mundo, pelo que o seu diagnóstico e prevenção se tornaram essenciais para a manutenção de um estado de saúde periodontal e para evitar em última instância a perda dentária. A origem da evolução da gengivite para periodontite é um fenómeno que ainda não obteve consenso, não sendo possível prever a progressão da doença.
O glico-OralOma da doença periodontal integra o conjunto de proteínas humanas glicosiladas existentes na cavidade oral associadas à doença periodontal. O presente estudo tem como finalidade elucidar os mecanismos moleculares e respetivas vias de sinalização que estão comprometidas nesta doença através da identificação das proteínas que participam na instauração da doença periodontal.
O estudo do proteoma salivar da doença periodontal permitiu a identificação de duas proteínas importantes que podem explicar a evolução da doença, e que se encontram alteradas, apresentando regulação aumentada. Uma delas é a proteína glicosilada zinc-alfa-2-glicoprotein (P25311), considerada um biomarcador tumoral e associada à obesidade. A sua inibição por TNF-alpha e diminuição da quantidade de expressão na periodontite crónica (-8,5) e o aumento significativo na periodontite agressiva (2,2), poderá torná-la num marcador da doença periodontal a considerar. A outra proteína potencialmente envolvida na doença periodontal é a prostaglandin E synthase (O14864), exclusiva da periodontite crónica, cuja produção é controlada por TNF-alpha e uma vez estimulada, pela COX-2, induz a produção de PGE2.
Os resultados obtidos provam o potencial dos estudos in silico permitindo, a partir da análise do proteoma salivar, estabelecer novos alvos para futuros estudos experimentais e identificação de potenciais biomarcadores salivares, visando a intervenção terapêutica, clínica ou de diagnóstico da doença periodontal.
Periodontal disease is a disease with high prevalence in the world, so it´s diagnosis and prevention have become essential for the maintenance of periodontal health status and ultimately to prevent tooth loss. The origin of the development of gingivitis to periodontitis is a phenomenon that has not yet reached consensus and it is not possible to predict disease progression. The glyco-OralOme of periodontal disease includes the set of glycosylated human proteins existing in the oral cavity associated with periodontal disease. The present study aims to elucidate the molecular mechanisms and respective signaling pathways that are compromised in this disease through the identification of proteins involved in the initiation of periodontal disease. The study of the salivary proteome of periodontal disease allowed the identification of two important proteins that can explain the evolution of the disease, and are altered with increased regulation. One is zinc-alpha 2- glicoprotein (P25311), a glycoprotein that is considered a tumor-associated obesity biomarker. Its downregulation by TNF - alpha and decreased amount of expression in chronic periodontitis (-8,5) and significant increase in aggressive periodontitis (2,2) can make it a marker for periodontal disease to consider. The other protein potentially involved in periodontitis is prostaglandin E synthase (O14864), exclusive of chronic periodontitis, whose production is controlled by TNF-alpha and once stimulated by COX-2, it induces the production of PGE2 . The obtained results prove the potential of in silico studies allowing, from the salivary proteome analysis, establish new targets for future experimental studies and identification of potential biomarkers salivary aiming at therapy intervention or medical diagnosis of periodontal disease.
Periodontal disease is a disease with high prevalence in the world, so it´s diagnosis and prevention have become essential for the maintenance of periodontal health status and ultimately to prevent tooth loss. The origin of the development of gingivitis to periodontitis is a phenomenon that has not yet reached consensus and it is not possible to predict disease progression. The glyco-OralOme of periodontal disease includes the set of glycosylated human proteins existing in the oral cavity associated with periodontal disease. The present study aims to elucidate the molecular mechanisms and respective signaling pathways that are compromised in this disease through the identification of proteins involved in the initiation of periodontal disease. The study of the salivary proteome of periodontal disease allowed the identification of two important proteins that can explain the evolution of the disease, and are altered with increased regulation. One is zinc-alpha 2- glicoprotein (P25311), a glycoprotein that is considered a tumor-associated obesity biomarker. Its downregulation by TNF - alpha and decreased amount of expression in chronic periodontitis (-8,5) and significant increase in aggressive periodontitis (2,2) can make it a marker for periodontal disease to consider. The other protein potentially involved in periodontitis is prostaglandin E synthase (O14864), exclusive of chronic periodontitis, whose production is controlled by TNF-alpha and once stimulated by COX-2, it induces the production of PGE2 . The obtained results prove the potential of in silico studies allowing, from the salivary proteome analysis, establish new targets for future experimental studies and identification of potential biomarkers salivary aiming at therapy intervention or medical diagnosis of periodontal disease.
Description
Keywords
Doença periodontal Gengivite Periodontite Biomarcadores Glico-Oraloma Periodontal disease Gingivitis Periodontitis Biomarkers Glico OralOme