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Objetivo: realizar análise histomorfométrica do tecido conjuntivo retirado do palato pela técnica de Harris (lâmina dupla). Materiais e Métodos: Foram submetidos a cirurgia de recobrimento radicular, 14 pacientes entre homens e mulheres, de 25 a 50 anos de idade, com recessões gengivais classes I eII de Miller. A amostra de tecido conjuntivo foi colhida da região compreendida entre distal de 1º molar superior e distal de canino superior, sendo obtidas de acordo com a técnica avaliada no estudo. Foi removido um tamanho além do necessário de tecido conjuntivo na cirurgia utilizada na pesquisa. Tecido epitelial e parte de tecido conjuntivo não utilizados foram armazenados em eppendorf com substância de formol a 10% para a análise histomorfométrica. No total, foram avaliadas 11 amostras. Todas as análises morfológicas foram realizadas por dois avaliadores independentes e calibrados, de maneira cega, sendo os dados compilados e avaliados estatisticamente por um terceiro avaliador independente. Resultados: Observamos que seis amostras não apresentaram restos de tecido epitelial, cinco apresentaram restos de tecido epitelial em uma fração da superfície do tecido conjuntivo, sendo que duas apresentaram restos de tecido epitelial ao longo de quase toda a extensão do tecido conjuntivo. Conclusão: Nem sempre há total eliminação do tecido epitelial após a remoção de enxerto de tecido conjuntivo subepitelial pela técnica de Harris com utilização de bisturi de lâmina dupla.
Objective: Perform a histomorphometric analysis of the connective tissue removed from the palate using the Harris technique (double blade). Materials and methods: 14 patients between men and women, 25 to 50 years of age, with Miller class I and II gingival recessions underwent root covering surgery. The connective tissue sample was collected from the region between the distal upper 1st molar and the distal upper canine, being obtained according to the technique evaluated in the study. An excess size of connective tissue was removed in the surgery used in the research. Unused epithelial tissue and connective tissue were stored in eppendorf with 10% formaldehyde for histomorphometric analysis. In total, 11 samples were evaluated. All morphological analyzes were performed by two independent and blindly calibrated evaluators, the data being compiled and evaluated statistically by a third independent evaluator. Results: We observed that six samples did not present remnants of epithelial tissue, five had remnants of epithelial tissue on a fraction of the surface of the connective tissue, and two had remnants of epithelial tissue along almost the entire length of the connective tissue. Conclusion: There is not always complete elimination of epithelial tissue after subepithelial connective tissue graft removal using the Harris technique using a double-blade scalpel.
Objective: Perform a histomorphometric analysis of the connective tissue removed from the palate using the Harris technique (double blade). Materials and methods: 14 patients between men and women, 25 to 50 years of age, with Miller class I and II gingival recessions underwent root covering surgery. The connective tissue sample was collected from the region between the distal upper 1st molar and the distal upper canine, being obtained according to the technique evaluated in the study. An excess size of connective tissue was removed in the surgery used in the research. Unused epithelial tissue and connective tissue were stored in eppendorf with 10% formaldehyde for histomorphometric analysis. In total, 11 samples were evaluated. All morphological analyzes were performed by two independent and blindly calibrated evaluators, the data being compiled and evaluated statistically by a third independent evaluator. Results: We observed that six samples did not present remnants of epithelial tissue, five had remnants of epithelial tissue on a fraction of the surface of the connective tissue, and two had remnants of epithelial tissue along almost the entire length of the connective tissue. Conclusion: There is not always complete elimination of epithelial tissue after subepithelial connective tissue graft removal using the Harris technique using a double-blade scalpel.
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Recobrimento radicular Enxerto tecido conjuntivo Plástica periodontal Histomorfometria de conjuntivo Morfologia de conjuntivo Root coverage Graft tissue Periodontal plastic surgery Connective tissue Histomorphometry Connective morphology