Purification and characterization of a novel trypsin inhibitor from solanum tuberosum subsp. andigenum var. overa : study of the expression levels and preliminary evaluation of its antimicrobial activity

Cisneros, José Sebastián; Cotabarren, Juliana; Parisi, Mónica Graciela; Vasconcelos, Marta; Obregón, Walter David

http://hdl.handle.net/10400.14/30512

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Authors

Cisneros, José Sebastián

Cotabarren, Juliana

Parisi, Mónica Graciela

Vasconcelos, Marta

Obregón, Walter David

Abstract(s)

Protease inhibitors (PIs) have been traditionally recognized by their potential biomedical application in events with exacerbation of endogenous proteases activity. Plant PIs have gained interest as naturally occurring molecules, which usually show lower environmental impact residual toxicity than synthetic compounds. In this work, we isolated, cloned, expressed and purified a novel trypsin inhibitor from S. tuberosum subsp. andigenum var overa, named oPTI. A significant over-expression of the oPTI coding gene after 48 h exposure of methyl jasmonate compared to the gene of reference. This inhibitor showed a molecular mass of 12 kDa and a Ki of 7.3 x10-7 M. Finally, we evaluated the antimicrobial activity of oPTI against different pathogenic microorganisms. The oPTI demonstrated inhibitory effect on the growth of Acinetobacter baumannii S-1, Acinetobacter baumannii R, Acinetobacter calcoaceticus R, Acinetobacter calcoaceticus S, Bacillus stearothermophilus, Escherichia coli, Pseudomonas aeruginosa, Salmonella braenderup, Salmonella enteritidis, Salmonella tiphymurium and Yersinia enterocolitica strains. This study represents the first report for the antimicrobial activity of a plant PI over a wide range of microorganisms. Our studies reinforce the importance of natural PIs as promising molecules for their potential application in the biomedical field and/or in the food industry as natural food preservatives.

Keywords

Solanum tuberosum Trypsin inhibitor Serine protease inhibitor Antimicrobial activity Andean potatoes

URI

http://hdl.handle.net/10400.14/30512

Citation

Cisneros, J. S., Cotabarren, J., Parisi, M. G., Vasconcelos, M. W., & Obregón, W. D. (2020). Purification and characterization of a novel trypsin inhibitor from solanum tuberosum subsp. andigenum var. overa : study of the expression levels and preliminary evaluation of its antimicrobial activity. International Journal of Biological Macromolecules