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- Biotechnological tools to fight against bursaphelenchus xilophilus on p. pinasterPublication . Vasconcelos, Marta W.
- Sargassum muticum and Osmundea pinnatifida enzymatic extracts: chemical, structural, and cytotoxic characterizationPublication . Rodrigues, Dina; Costa-Pinto, Ana R.; Sousa, Sérgio; Vasconcelos, Marta W.; Pintado, Manuela M.; Pereira, Leonel; Rocha-Santos, Teresa A. P.; Costa, João P. da; Silva, Artur M. S.; Duarte, Armando C.; Gomes, Ana M. P.; Freitas, Ana C.Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts. Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts. Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2–27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4–2.7 g/100gprotein) characterized both extracts. FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida. No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts. The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.
- Produção diferencial de compostos voláteis em caules de Pinus pinaster inoculados com o nemátode da madeira do pinheiro (Bursaphelenchus xylophilus)Publication . Lima, Marta R. M.; Ferreira, António C. Silva; Vasconcelos, Marta W.
- Novos projetos para melhorar a qualidade e aumentar o consumo das leguminosas na EuropaPublication . Vasconcelos, Marta W.
- Morpho-physiological parameters affecting iron deficiency chlorosis in soybean (Glycine max L.)Publication . Vasconcelos, Marta; Grusak, Michael A.Background and aims Iron deficiency chlorosis (IDC) leads to severe leaf chlorosis, low photosynthetic rates, and yield reductions of several million metric tonnes each year. In order to devise breeding and genetic transformation programs that aim at generating high-yielding and IDC-tolerant soybean lines, it is necessary to better understand the mechanisms that enable tolerant plants to survive under Fe-limiting conditions. Methods An in silico analysis in the USDA soybean collection allowed the identification of a set of novel efficient and inefficient soybean cultivars which can be used in future studies concerning IDC response. Plants were grown in iron deficient and iron sufficient conditions using a bicarbonate system and several IDCrelated aspects were studied. Results A new set of efficient and inefficient soybean lines were identified in silico, and their tolerance to IDC was confirmed under laboratorial conditions. New plant traits that are highly correlated to IDC scoring were identified: a negative correlation was found between SPADvalues and stemweight,weight of the unifoliolates and iron concentration of the first unifoliolates was found; higher SPAD values were correlated with the amount of iron in the first trifoliate leaves. Our data also show that having higher concentrations of iron in the seeds provides increased resistance to IDC. No correlation was found between root iron reductase activity and chlorosis. Conclusions Soybean differential chlorosis susceptibility between different accessions is linked to specific morpho-physiological parameters such as unifoliolate leaf size, stem weigh, concentration of iron in the seeds, and tissue iron partitioning.
- Como produzir alimentos transgénicos com maior valor nutritivo?Publication . Vasconcelos, Marta
- Nutrição nas leguminosas através da biofortificaçãoPublication . Santos, Cátia; Vasconcelos, Marta W.
- For the love of wise agronomy: transition paths to legume supported food- and feed-systems in EuropePublication . Iannetta, Pietro; Balázs, Bálint; Davies, Chantel; Hamann, Karen; Debeljak, Marko; Hamann, Karen; Williams, Michael; Vasconcelos, Marta
- Physiological and phytochemical quality of ready-to-eat rocket leaves as affected by processing, modified atmosphere and storage temperaturePublication . Amaro, Ana Luísa; Pereira, Maria João; Carvalho, Susana; Vasconcelos, Marta; Pintado, Maria ManuelaRocket leaves are widely consumed as a ready-to-eat (RTE) leafy vegetable, valued by its wide range of health promoting phytonutrients, including vitamin C and phenolic compounds. Processing and storage conditions are known to affect quality of RTE rocket leaves, with package atmosphere and low temperature as key factors in quality maintenance throughout processing to consumption. However, information on rocket leaves nutritional quality as affected by modified atmosphere and storage temperature is scarce. In order to generate recommendations to optimize phytochemical preservation of rocket leaves during processing, a nutritional audit of the processing line of a fresh-cut vegetables enterprise was performed. Samples were collected at each step of the production flowchart of RTE rocket-leaves: at rocket leaves reception, after washing and decontamination, after centrifugation and after packaging. All samples were analyzed for nutritional characterization. Furthermore, the effect of modified atmosphere and temperature conditions during storage on physiological and phytochemical quality of RTE rocket leaves was evaluated and the relative changes determined. To study modified atmosphere effects on quality, freshly harvested rocket leaves were processed, packaged in low oxygen permeability film bags, flushed with different oxygen concentrations (2.5; 5; 10 and 20% O2), thermo sealed with a vacuum packing machine and to analyze the effect of temperature on quality, freshly harvested rocket leaves were processed, packaged and stored at different temperatures (0, 5 and 10 °C) for 14 days. All rocket leaves samples were assessed for respiration rate, chlorophyll content and phytochemical composition during storage. Processing, modified atmosphere and storage temperature significantly affected respiration rate, chlorophyll content and phytochemical composition of RTE rocket leaves. The integration of these results contributes to the understanding of the effects of processing and storage conditions on overall quality of RTE vegetables and provide useful information for developing processes aimed at the modulation of nutritional quality and shelf-life extension.
- Phenotypic evaluation of the IDC profile of soybean plants after inoculation with plant growth-promoting bacteriaPublication . Castro, Paula M. L.; Carvalho, Susana M. P.; Vasconcelos, Marta W.; Roriz, Mariana