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- Biotechnological tools to fight against bursaphelenchus xilophilus on p. pinasterPublication . Vasconcelos, Marta W.
- Sargassum muticum and Osmundea pinnatifida enzymatic extracts: chemical, structural, and cytotoxic characterizationPublication . Rodrigues, Dina; Costa-Pinto, Ana R.; Sousa, Sérgio; Vasconcelos, Marta W.; Pintado, Manuela M.; Pereira, Leonel; Rocha-Santos, Teresa A. P.; Costa, João P. da; Silva, Artur M. S.; Duarte, Armando C.; Gomes, Ana M. P.; Freitas, Ana C.Seaweeds, which have been widely used for human consumption, are considered a potential source of biological compounds, where enzyme-assisted extraction can be an efficient method to obtain multifunctional extracts. Chemical characterization of Sargassum muticum and Osmundea pinnatifida extracts obtained by Alcalase and Viscozyme assisted extraction, respectively, showed an increment of macro/micro elements in comparison to the corresponding dry seaweeds, while the ratio of Na/K decreased in both extracts. Galactose, mannose, xylose, fucose, and glucuronic acid were the main monosaccharides (3.2–27.3 mg/glyophilized extract) present in variable molar ratios, whereas low free amino acids content and diversity (1.4–2.7 g/100gprotein) characterized both extracts. FTIR-ATR and 1H NMR spectra confirmed the presence of important polysaccharide structures in the extracts, namely fucoidans from S. muticum or agarans as sulfated polysaccharides from O. pinnatifida. No cytotoxicity against normal mammalian cells was observed from 0 to 4 mglyophilized extract/mL for both extracts. The comprehensive characterization of the composition and safety of these two extracts fulfils an important step towards their authorized application for nutritional and/or nutraceutical purposes.
- Produção diferencial de compostos voláteis em caules de Pinus pinaster inoculados com o nemátode da madeira do pinheiro (Bursaphelenchus xylophilus)Publication . Lima, Marta R. M.; Ferreira, António C. Silva; Vasconcelos, Marta W.
- Novos projetos para melhorar a qualidade e aumentar o consumo das leguminosas na EuropaPublication . Vasconcelos, Marta W.
- Como produzir alimentos transgénicos com maior valor nutritivo?Publication . Vasconcelos, Marta
- Nutrição nas leguminosas através da biofortificaçãoPublication . Santos, Cátia; Vasconcelos, Marta W.
- For the love of wise agronomy: transition paths to legume supported food- and feed-systems in EuropePublication . Iannetta, Pietro; Balázs, Bálint; Davies, Chantel; Hamann, Karen; Debeljak, Marko; Hamann, Karen; Williams, Michael; Vasconcelos, Marta
- Physiological and phytochemical quality of ready-to-eat rocket leaves as affected by processing, modified atmosphere and storage temperaturePublication . Amaro, Ana Luísa; Pereira, Maria João; Carvalho, Susana; Vasconcelos, Marta; Pintado, Maria ManuelaRocket leaves are widely consumed as a ready-to-eat (RTE) leafy vegetable, valued by its wide range of health promoting phytonutrients, including vitamin C and phenolic compounds. Processing and storage conditions are known to affect quality of RTE rocket leaves, with package atmosphere and low temperature as key factors in quality maintenance throughout processing to consumption. However, information on rocket leaves nutritional quality as affected by modified atmosphere and storage temperature is scarce. In order to generate recommendations to optimize phytochemical preservation of rocket leaves during processing, a nutritional audit of the processing line of a fresh-cut vegetables enterprise was performed. Samples were collected at each step of the production flowchart of RTE rocket-leaves: at rocket leaves reception, after washing and decontamination, after centrifugation and after packaging. All samples were analyzed for nutritional characterization. Furthermore, the effect of modified atmosphere and temperature conditions during storage on physiological and phytochemical quality of RTE rocket leaves was evaluated and the relative changes determined. To study modified atmosphere effects on quality, freshly harvested rocket leaves were processed, packaged in low oxygen permeability film bags, flushed with different oxygen concentrations (2.5; 5; 10 and 20% O2), thermo sealed with a vacuum packing machine and to analyze the effect of temperature on quality, freshly harvested rocket leaves were processed, packaged and stored at different temperatures (0, 5 and 10 °C) for 14 days. All rocket leaves samples were assessed for respiration rate, chlorophyll content and phytochemical composition during storage. Processing, modified atmosphere and storage temperature significantly affected respiration rate, chlorophyll content and phytochemical composition of RTE rocket leaves. The integration of these results contributes to the understanding of the effects of processing and storage conditions on overall quality of RTE vegetables and provide useful information for developing processes aimed at the modulation of nutritional quality and shelf-life extension.
- Phenotypic evaluation of the IDC profile of soybean plants after inoculation with plant growth-promoting bacteriaPublication . Castro, Paula M. L.; Carvalho, Susana M. P.; Vasconcelos, Marta W.; Roriz, Mariana
- The effect of sprouting in lentil (lens culinaris) nutritional and microbiological profilePublication . Santos, Carla S.; Silva, Beatriz; Valente, Luisa M. P.; Gruber, Sabine; Vasconcelos, Marta W.Biological and vegetarian raw food products, in particular based on legume sprouts, are an increasing food trend, due to their improved nutritional value when compared to seeds. Herein, protein and mineral profiles were studied in 12 lentil varieties, with varieties Du Puy, Kleine Schwarze, Rosana, Flora, Große Rote and Kleine Späths II demonstrating the highest protein percentages. After sprouting, protein percentages increased significantly in 10 of the 12 varieties, with the highest increases ranging between 20–23% in Dunkelgrün Marmorierte, Du Puy, Große Rote and Kleine Späths II varieties. While Fe concentration was significantly decreased in three varieties (Samos, Große Rote and Kleine Späths II), Zn and Mn were positively impacted by sprouting (p ≤ 0.05). Magnesium concentration was not affected by sprouting, while Ca and K had percentage increases between 41% and 58%, and 28% and 30%, respectively, in the best performing varieties (Kleine Schwarze, Dunkelgrün Marmorierte, Samos and Rosana). Regardless of the associated nutritional benefits, issues pertaining to sprouts microbiological safety must be ensured. The best results for the disinfection protocols were obtained when combining the seed treatment with SDS reagent followed by an Amukine application on the sprouts, which did not affect germination rates or sprout length. The increasing levels of sprout consumption throughout the world require efficient implementation of safety measures, as well as a knowledge-based selection for the nutritional quality of the seeds.