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Purification and characterization of a novel trypsin inhibitor from solanum tuberosum subsp. andigenum var. overa : study of the expression levels and preliminary evaluation of its antimicrobial activity

2020Journal article Restricted access
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dc.contributor.authorCisneros, José Sebastián
dc.contributor.authorCotabarren, Juliana
dc.contributor.authorParisi, Mónica Graciela
dc.contributor.authorVasconcelos, Marta
dc.contributor.authorObregón, Walter David
dc.date.accessioned2020-05-06T15:03:34Z
dc.date.available2020-05-06T15:03:34Z
dc.date.issued2020
dc.description.abstractProtease inhibitors (PIs) have been traditionally recognized by their potential biomedical application in events with exacerbation of endogenous proteases activity. Plant PIs have gained interest as naturally occurring molecules, which usually show lower environmental impact residual toxicity than synthetic compounds. In this work, we isolated, cloned, expressed and purified a novel trypsin inhibitor from S. tuberosum subsp. andigenum var overa, named oPTI. A significant over-expression of the oPTI coding gene after 48 h exposure of methyl jasmonate compared to the gene of reference. This inhibitor showed a molecular mass of 12 kDa and a Ki of 7.3 x10-7 M. Finally, we evaluated the antimicrobial activity of oPTI against different pathogenic microorganisms. The oPTI demonstrated inhibitory effect on the growth of Acinetobacter baumannii S-1, Acinetobacter baumannii R, Acinetobacter calcoaceticus R, Acinetobacter calcoaceticus S, Bacillus stearothermophilus, Escherichia coli, Pseudomonas aeruginosa, Salmonella braenderup, Salmonella enteritidis, Salmonella tiphymurium and Yersinia enterocolitica strains. This study represents the first report for the antimicrobial activity of a plant PI over a wide range of microorganisms. Our studies reinforce the importance of natural PIs as promising molecules for their potential application in the biomedical field and/or in the food industry as natural food preservatives.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCisneros, J. S., Cotabarren, J., Parisi, M. G., Vasconcelos, M. W., & Obregón, W. D. (2020). Purification and characterization of a novel trypsin inhibitor from solanum tuberosum subsp. andigenum var. overa : study of the expression levels and preliminary evaluation of its antimicrobial activity. International Journal of Biological Macromoleculespt_PT
dc.identifier.doi10.1016/j.ijbiomac.2020.04.217pt_PT
dc.identifier.eid85084808616
dc.identifier.eissn1879-0003
dc.identifier.issn0141-8130
dc.identifier.pmid32360201
dc.identifier.urihttp://hdl.handle.net/10400.14/30512
dc.identifier.wos000564490100011
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPICT-2016-4365pt_PT
dc.subjectSolanum tuberosumpt_PT
dc.subjectTrypsin inhibitorpt_PT
dc.subjectSerine protease inhibitorpt_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectAndean potatoespt_PT
dc.titlePurification and characterization of a novel trypsin inhibitor from solanum tuberosum subsp. andigenum var. overa : study of the expression levels and preliminary evaluation of its antimicrobial activitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleInternational Journal of Biological Macromoleculespt_PT
person.familyNameVasconcelos
person.givenNameMarta
person.identifier74696
person.identifier.orcid0000-0002-5110-7006
person.identifier.ridI-8166-2013
person.identifier.scopus-author-id14627797100
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationdf60ebb4-3b71-4733-a9e3-bfa4687f5576
relation.isAuthorOfPublication.latestForDiscoverydf60ebb4-3b71-4733-a9e3-bfa4687f5576

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