Loading...
Publication
O microbioma associado à lesão de cárie radicular difere do microbioma oral recolhido no esmalte saudável do mesmo indivíduo?
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 3.79 MB | Adobe PDF |
Authors
Abstract(s)
Introdução: A cárie radicular é uma condição cada vez mais frequente em populações idosas, associada a fatores como idade, higiene oral, medicação e alterações na composição do microbioma oral. A compreensão da dinâmica bacteriana associada a estas lesões pode contribuir para estratégias preventivas mais eficazes. Objetivos: Comparar o perfil microbiológico de lesões de cárie radicular com o biofilme oral saudável do mesmo indivíduo, focando-se nas espécies Streptococcus mutans, Actinomyces naeslundii e Streptococcus mitis. Materiais e métodos: Foram recolhidas amostras de saliva, biofilme oral saudável e biofilme da lesão de cárie radicular em indivíduos com idade igual ou superior a 65 anos. A quantificação bacteriana foi realizada através de qRT-PCR, permitindo avaliar a carga relativa de cada espécie nos diferentes nichos. Resultados: Verificou-se uma variabilidade interindividual significativa. Algumas amostras apresentaram menor carga bacteriana nas lesões, enquanto noutras se observou maior concentração de espécies cariogénicas na zona afetada. S. mitis demonstrou uma presença mais estável entre os dois ambientes, sugerindo maior resiliência ecológica. Conclusão: Os resultados sugerem que o microbioma presente em lesões de cárie radicular pode diferir substancialmente do microbioma oral saudável, refletindo alterações locais ou sistémicas. Este estudo reforça a importância de considerar a dinâmica microbiana na avaliação do risco de cárie em populações envelhecidas.
Introduction: Root caries is an increasingly prevalent condition in older adults, associated with age-related factors such as oral hygiene, medication use, and changes in the oral microbiome. Understanding bacterial dynamics in these lesions may support more effective preventive strategies. Goal: To compare the microbial profile of root caries lesions with the healthy oral biofilm from the same individual, focusing on Streptococcus mutans, Actinomyces naeslundii, and Streptococcus mitis. Materials and methods: Samples of saliva, healthy oral biofilm, and root caries lesion biofilm were collected from individuals aged 65 years or older. Bacterial quantification was performed using qRT-PCR to assess the relative load of each species in the different ecological niches. Results: Substantial interindividual variability was observed. In some cases, lower bacterial loads were found in the lesions, while in others, higher concentrations of cariogenic species were detected. S. mitis showed a more consistent presence across both environments, suggesting greater ecological resilience. Conclusion: The microbiome associated with root caries may differ considerably from the healthy oral microbiome, reflecting potential local or systemic imbalances. This study highlights the importance of microbial dynamics in assessing caries risk, particularly in elderly populations.
Introduction: Root caries is an increasingly prevalent condition in older adults, associated with age-related factors such as oral hygiene, medication use, and changes in the oral microbiome. Understanding bacterial dynamics in these lesions may support more effective preventive strategies. Goal: To compare the microbial profile of root caries lesions with the healthy oral biofilm from the same individual, focusing on Streptococcus mutans, Actinomyces naeslundii, and Streptococcus mitis. Materials and methods: Samples of saliva, healthy oral biofilm, and root caries lesion biofilm were collected from individuals aged 65 years or older. Bacterial quantification was performed using qRT-PCR to assess the relative load of each species in the different ecological niches. Results: Substantial interindividual variability was observed. In some cases, lower bacterial loads were found in the lesions, while in others, higher concentrations of cariogenic species were detected. S. mitis showed a more consistent presence across both environments, suggesting greater ecological resilience. Conclusion: The microbiome associated with root caries may differ considerably from the healthy oral microbiome, reflecting potential local or systemic imbalances. This study highlights the importance of microbial dynamics in assessing caries risk, particularly in elderly populations.
Description
Keywords
Cárie radicular Idosos Microbioma Espécies cariogénicas Espécies carioprotetoras Root caries Elderly Microbiome Cariogenic species Carioprotective species
Pedagogical Context
Citation
Publisher
CC License
Without CC licence