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Authors
Abstract(s)
Objetivos: O presente estudo teve como objetivo comparar as propriedades
mecânicas de resistência à tração e estrutura entre as membranas produzidas
por A-PRF (Advanced- Platelet Rich Fibrin) e A-PRF+ (Advanced-Platelet Rich
Fibrin+).
Materiais e métodos: Recorrendo à colheita de sangue de um dador saudável
sem história de uso de anticoagulantes ou imunossupressores, realizou-se a
preparação das membranas seguindo as indicações protocolares definidas na
literatura para A-PRF e A-PRF+. De um N=16/grupo, 13 membranas de A-PRF
e 12 de A-PRF+ foram submetidas ao teste de tração, para a obtenção de valores
referentes à tração máxima e tração média. Os dados obtidos foram analisados
estatisticamente com o teste t não pareado. Após avaliação desta variável, as
membranas foram observadas em MEV (Microscopia Eletrónica de Varredura).
Resultados: Foram obtidos em relação à tração máxima, 0.0020 N.mm-2 para o
A-PRF e 0.0022 N.mm-2 para A-PRF+. Relativamente à tração média, A-PRF
obteve 0.0012 N.mm-2
, enquanto o A-PRF+ obteve 0.0015 N.mm-2
(p˂0,01). Nas
observações de superfície com MEV, A-PRF+ mostrou ser o concentrado
plaquetário mais poroso, com maior abundância de fibras e preservação celular.
Conclusão: Este estudo permitiu concluir que o protocolo A-PRF+ foi capaz de
produzir membranas com valores de tração máxima superiores aos obtidos pelo
A-PRF, sendo os resultados indicativo de que o protocolo apresentou
membranas com maior resistência e capacidade elástica ao serem tracionadas
por duas forças opostas. A este fenómeno acrescenta-se a arquitetura
demonstrada na matriz de A-PRF+ e as propriedades biológicas otimizadas
descritas na literatura.
Purpose: This study aimed to formulate a comparison of the mechanical properties of tensile strength and structural organization between membranes produced by A-PRF (Advanced Platelet-Rich Fibrin) and A-PRF+ (Advanced Platelet-Rich Fibrin+). Materials and Methods: Blood was collected from a healthy donor with no history of anticoagulant or immunosuppressant use, the membranes were prepared following the protocol indications defined in the literature for A-PRF and A-PRF+. From an N=16 for each protocol, 13 membranes of A-PRF and 12 of APRF+ were submitted to the traction test, evaluating maximum and average traction. Data was statistically analyzed using the unpaired t test. Membranes were then carefully observed in SEM (Scanning Electron Microscopy). Results: For maximum traction were obtained 0.0020 for A-PRF and 0.0022 for A-PRF+. Regarding the average traction, A-PRF scored 0.0012 while A-PRF+ obtained 0.0015 (p=0.01 unpaired t-test). Surface morphology observations with SEM, A-PRF+ showed to be the most porous platelet concentrate, with greatest fiber abundance and cell preservation. Conclusion: This study allowed to conclude that A-PRF+ protocol was able to produce membranes with higher maximum traction results than those found for A-PRF, indicating that the protocol with low centrifugation time, presented membranes with better viscoelastic strength when they are stretched by two opposed forces. To this phenomenon is added the architecture demonstrated in the A-PRF+ matrix and the optimized biological properties described in literature. A-PRF+, by the view of the developed findings in this work, a better option compared to A-PRF. Keywords: “Platelet-Rich Fibrin”, “Viscoelastic”, “Tensile Strength”, “Rupture”, “Porosity”, “Low-Speed Centrifugation Concept”.
Purpose: This study aimed to formulate a comparison of the mechanical properties of tensile strength and structural organization between membranes produced by A-PRF (Advanced Platelet-Rich Fibrin) and A-PRF+ (Advanced Platelet-Rich Fibrin+). Materials and Methods: Blood was collected from a healthy donor with no history of anticoagulant or immunosuppressant use, the membranes were prepared following the protocol indications defined in the literature for A-PRF and A-PRF+. From an N=16 for each protocol, 13 membranes of A-PRF and 12 of APRF+ were submitted to the traction test, evaluating maximum and average traction. Data was statistically analyzed using the unpaired t test. Membranes were then carefully observed in SEM (Scanning Electron Microscopy). Results: For maximum traction were obtained 0.0020 for A-PRF and 0.0022 for A-PRF+. Regarding the average traction, A-PRF scored 0.0012 while A-PRF+ obtained 0.0015 (p=0.01 unpaired t-test). Surface morphology observations with SEM, A-PRF+ showed to be the most porous platelet concentrate, with greatest fiber abundance and cell preservation. Conclusion: This study allowed to conclude that A-PRF+ protocol was able to produce membranes with higher maximum traction results than those found for A-PRF, indicating that the protocol with low centrifugation time, presented membranes with better viscoelastic strength when they are stretched by two opposed forces. To this phenomenon is added the architecture demonstrated in the A-PRF+ matrix and the optimized biological properties described in literature. A-PRF+, by the view of the developed findings in this work, a better option compared to A-PRF. Keywords: “Platelet-Rich Fibrin”, “Viscoelastic”, “Tensile Strength”, “Rupture”, “Porosity”, “Low-Speed Centrifugation Concept”.
Description
Keywords
Fibrina-rica em plaquetas Viscoelástica Teste de tensão Porosidade Rotura Platelet-rich fibrin Viscoelastic Tensile strength Rupture Porosity Low-speed centrifugation concept