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Avaliação dos níveis de NAMPT na saliva de pacientes com doença periodontal
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Abstract(s)
Introdução: A periodontite é uma doença inflamatória crónica que afeta os tecidos de suporte dentário, tendo impacto significativo na qualidade de vida dos pacientes. O diagnóstico precoce é essencial para travar a sua progressão. A nova classificação introduzida em 2018 introduziu critérios por estágios e graus, realçando fatores de risco como tabagismo e diabetes. Biomarcadores salivares, como a visfatina (NAMPT) surgem como ferramentas promissoras para o diagnóstico não invasivo. Objetivo: Avaliar a visfatina/NAMPT como biomarcador salivar da periodontite, relacionando os seus níveis com a gravidade clínica e fatores de risco. Material e métodos: Estudo observacional com 32 pacientes diagnosticados na consulta de periodontologia na clínica da FMD-UCP. Foram recolhidos dados clínicos e amostras de saliva e biofilme. A quantificação de NAMPT na saliva foi feita por ELISA. Resultados: Este estudo avaliou os níveis de NAMPT salivar em 19 indivíduos com diferentes estados de saúde periodontal. Os valores mais elevados foram encontrados nos grupos de indivíduos com periodontite. Fatores sistémicos e modificáveis, como o tabagismo e a escovagem dos dentes menos frequente, associaram-se a concentrações superiores de NAMPT. Conclusão: Observou-se uma tendência de aumento dos níveis salivares de NAMPT e a gravidade da periodontite, sugerindo o seu valor como biomarcador inflamatório. Este trabalho potencia novas abordagens de diagnóstico não invasivo e gestão dos pacientes com doença periodontal.
Introduction: Periodontitis is a chronic inflammatory disease affecting thooth-supporting tissues, with significant impact on quality of life of patients. Early diagnosis is crucial to halt its progression. The 2018 classification introduced staging and grading, emphasizing risk factors like smoking and diabetes. Salivary biomarkers, such as visfatina (NAMPT), show promise for non-invasive diagnostics. Objective: To assess salivary visfatin/NAMPT as a biomarker of periodontal disease, integrating clinical data, risk factors, and diagnostic support technologies. Materials and methods: Observational study involving 32 patients diagnosed at the periodontology consultation at the FMD-UCP clinic. Clinical data, as well as saliva and biofilm samples, were collected. The quantification of NAMPT in saliva was performed using ELISA. Results: This study assessed salivary NAMPT levels in 19 individuals with different periodontal health statuses. Higher levels were observed in the group with periodontitis. Systemic and modifiable factors such as smoking and less frequent thoothbrushing were associated with increased concentrations. Conclusion: An increasing trend in salivary NAMPT levels was observed with the severity of periodontitis, suggesting its value as an inflammatory biomarker. This work supports approaches for non-invasive diagnosis and management of patients with periodontial disease.
Introduction: Periodontitis is a chronic inflammatory disease affecting thooth-supporting tissues, with significant impact on quality of life of patients. Early diagnosis is crucial to halt its progression. The 2018 classification introduced staging and grading, emphasizing risk factors like smoking and diabetes. Salivary biomarkers, such as visfatina (NAMPT), show promise for non-invasive diagnostics. Objective: To assess salivary visfatin/NAMPT as a biomarker of periodontal disease, integrating clinical data, risk factors, and diagnostic support technologies. Materials and methods: Observational study involving 32 patients diagnosed at the periodontology consultation at the FMD-UCP clinic. Clinical data, as well as saliva and biofilm samples, were collected. The quantification of NAMPT in saliva was performed using ELISA. Results: This study assessed salivary NAMPT levels in 19 individuals with different periodontal health statuses. Higher levels were observed in the group with periodontitis. Systemic and modifiable factors such as smoking and less frequent thoothbrushing were associated with increased concentrations. Conclusion: An increasing trend in salivary NAMPT levels was observed with the severity of periodontitis, suggesting its value as an inflammatory biomarker. This work supports approaches for non-invasive diagnosis and management of patients with periodontial disease.
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Keywords
Periodontite Nicotamide phosphoribosyltransferase Biomarcadores Enzyme-linked immunosorbent assay Progressão da doença Periodontitis Biomarkers Saliva Diagnosis disease progression
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