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Authors
Abstract(s)
O tratamento endodôntico tem como objetivo, otimizar a desinfeção do canal
radicular e prevenir a reinfeção. Os microrganismos predominantes em dentes
tratados com sintomatologia são Enterococcus faecalis, Fusobacterium
nucleatum e do reino Fungi a Candida albicans.
Os oceanos contêm inúmeros habitats específicos diferentes, sendo
necessário que os microrganismos desenvolvam diferentes mecanismos de
adaptação para sobreviverem. Alguns destes são a produção de moléculas e
enzimas específicas que lhes conferem propriedades antibacterianas,
antifúngicas, anti-inflamatórias, antiprotozoárias, antivirais, anticancerígenas...
Este trabalho tem como objetivo determinar se extratos de fungos marinhos,
(extratos de Penicillium lusitanum e de Aspergillus affinis) são eficazes na
eliminação de Enterococcus faecalis e de Candida albicans durante o processo
de irrigação endodôntica. Três concentrações dos dois extratos de fungos
marinhos foram preparadas e testadas. Protocolos de produção, extração,
difusão em disco e de viabilidade celular foram seguidos de forma a obter
resultados quanto à inibição e à viabilidade celular que estes extratos de fungos
são capazes de produzir em E. faecalis e C. albicans.
Analisando os resultados, é percetível que os extratos de fungos de P.
lusitanum e A. affinis se mostraram eficazes quando usados de forma isolada na
inibição de C. albicans e de E. faecalis, respetivamente, o que são resultados
animadores para a continuação desta investigação. Com esta investigação foi
ainda possível abrir novos possíveis caminhos para a utilização dos extratos de
fungos de P. lusitanum noutras áreas da medicina, como por exemplo no
tratamento de Candidíase.
Endodontic treatment aims to optimize root canal disinfection and prevent reinfection. The predominant microorganisms in teeth treated with symptoms are Enterococcus faecalis, Fusobacterium nucleatum and from the kingdom Fungi to Candida albicans. The oceans contain many different specific habitats, requiring microorganisms to develop different adaptation mechanisms to survive. Some of these are the production of specific biomolecules and enzymes that give them antibacterial, antifungal, anti-inflammatory, antiprotozoal, antiviral, anticancer properties... This work aims to determine whether extracts of marine fungi (Penicillium lusitanum and Aspergillus affinis extracts) during the irrigation process are effective in eliminating Enterococcus faecalis and Candida albicans. Three concentrations of the two marine fungus extracts were prepared and tested. Production, extraction, disk diffusion and cell viability protocols were followed in order to obtain results regarding the inhibition and cell viability that these fungal strata are capable of producing in E. faecalis and C. albicans. Analysing the results, it is noticeable that the fungal extracts of P. lusitanum and A. affinis proved to be effective when used alone in inhibiting C. albicans and E. faecalis, respectively, which are encouraging results for the continuation of this investigation. With this research it was also possible to open new possible ways for the use of fungal extracts of P. lusitanum in other areas of medicine, such as in the treatment of Candidiasis.
Endodontic treatment aims to optimize root canal disinfection and prevent reinfection. The predominant microorganisms in teeth treated with symptoms are Enterococcus faecalis, Fusobacterium nucleatum and from the kingdom Fungi to Candida albicans. The oceans contain many different specific habitats, requiring microorganisms to develop different adaptation mechanisms to survive. Some of these are the production of specific biomolecules and enzymes that give them antibacterial, antifungal, anti-inflammatory, antiprotozoal, antiviral, anticancer properties... This work aims to determine whether extracts of marine fungi (Penicillium lusitanum and Aspergillus affinis extracts) during the irrigation process are effective in eliminating Enterococcus faecalis and Candida albicans. Three concentrations of the two marine fungus extracts were prepared and tested. Production, extraction, disk diffusion and cell viability protocols were followed in order to obtain results regarding the inhibition and cell viability that these fungal strata are capable of producing in E. faecalis and C. albicans. Analysing the results, it is noticeable that the fungal extracts of P. lusitanum and A. affinis proved to be effective when used alone in inhibiting C. albicans and E. faecalis, respectively, which are encouraging results for the continuation of this investigation. With this research it was also possible to open new possible ways for the use of fungal extracts of P. lusitanum in other areas of medicine, such as in the treatment of Candidiasis.
Description
Keywords
Endodontia Irrigação Desinfeção intracanalar Fungos marinhos Endodontics Irrigation Intracanal disinfection Marine fungi