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Authors
Abstract(s)
Introdução: A periodontite é uma doença complexa, com vários fatores
etiológicos, que se desenvolve ao longo do tempo, através de acumulação de
placa dentária e de disbiose bacteriana. Tem como principal terapia adjuvante a
antibioticoterapia, que tem revelado problemas relativamente a resistência
bacteriana. Como tal, a terapia fotodinâmica antimicrobiana (aPDT) tem sido
estudada como potencial substituta da antibioticoterapia, como terapia
adjuvante. Neste trabalho, pretende-se estudar o potencial de uma nova
abordagem baseada da aPDT usando porfirinas como fotossensibilizadores e o
fotopolimerizador, equipamento disponível em todos os consultórios de medicina
dentária como fonte de luz.
Material e métodos: Realizaram-se ensaios in vitro e ex vivo em dentes de
fotoinativação de microrganismos periodontopatogénicos (bactéria E. faecalis e
o fungo C. albicans) usando porfirinas como fotossensibilizador e o
fotopolimerizador como fonte de luz. Para estes estudos, foi selecionada a
ProtoIX como fotossensibilizador (testada a 10 e 20 μM, nos ensaios in vitro e a
20 e 50 μM nos ensaios ex vivo) e o fotopolimerizador como fonte de luz.
Resultados: Nos ensaios in vitro, foram observados decréscimos da viabilidade
do fungo C. albicans e da bactéria E. faecalis superiores a 3 log UFC/mL e a 6
log UFC/mL, respetivamente, quando expostos a uma concentração de 20 µM
de ProtoIX e 20 min de irradiação com luz do fotopolimerizador. Nos ensaios ex
vivo, apenas se verificou um ligeiro decréscimo na sobrevivência do fungo C.
albicans (0,8 log UFC/mL), após a incubação com 20 μM de ProtoIX e 20 min de
irradiação com luz do fotopolimerizador.
Conclusão: Este estudo comprova a elevada potencialidade da aPDT para a
inativação de microrganismos periodontopatogénicos usando a ProtoIX como
fotossensibilizador e o fotopolimerizador como fonte de luz. No entanto, mais
estudos deverão ser realizados de forma a otimizar este protocolo para a sua
aplicação em ambiente clínico.
Introduction: Periodontitis is a complex disease, with multiple etiologic factors, which develops through time, due to dental plaque accumulation and bacterial dysbiosis. Antibiotic therapy is the most used adjuvant therapy, which have demonstrated having issues related to bacterial resistance. So, antimicrobial PhotoDynamic Therapy (aPDT) has been studied as a potential substitute for antibiotic therapy, as an adjuvant therapy. In this work, it is intended to study the potential of a new approach using aPDT with protoporphyrin IX as photosensitizer and dental curing light, equipment which is available in every dental clinic, as light source. Material and methods: We have performed photoinactivation of periodontopathogens (Enterococcus faecalis bacteria and Candida albicans fungi) studies in vitro and ex vivo in teeth using porphyrins as photosensitizer and dental curing light as light source. For these studies, we have selected ProtoIX as photosensitizer (tested at 10 and 20 µM in vitro and at 20 and 50 µM) and dental curing light as light source. Results: In in vitro studies, we have observed viability decrease on C. albicans fungi and E. faecalis bacteria, above 3 log UFC/mL and 6 log UFC/mL, respectively, when exposed with a ProtoIX concentration of 20 µM and 20 minutes of dental curing light irradiation. In ex vivo studies, we have observed a slight decrease of C. albicans fungi survival (0.8 log UFC/mL), after its incubation on 20 µM of ProtoIX and 20 minutes irradiation, with a dental curing light. Conclusion: This study has shown aPDT’s high potential of periodontopathogens inactivation using ProtoIX as photosensitizer and dental curing light as light source. However, several more studies must be conducted to improve this protocol, so it can be used in clinical environments.
Introduction: Periodontitis is a complex disease, with multiple etiologic factors, which develops through time, due to dental plaque accumulation and bacterial dysbiosis. Antibiotic therapy is the most used adjuvant therapy, which have demonstrated having issues related to bacterial resistance. So, antimicrobial PhotoDynamic Therapy (aPDT) has been studied as a potential substitute for antibiotic therapy, as an adjuvant therapy. In this work, it is intended to study the potential of a new approach using aPDT with protoporphyrin IX as photosensitizer and dental curing light, equipment which is available in every dental clinic, as light source. Material and methods: We have performed photoinactivation of periodontopathogens (Enterococcus faecalis bacteria and Candida albicans fungi) studies in vitro and ex vivo in teeth using porphyrins as photosensitizer and dental curing light as light source. For these studies, we have selected ProtoIX as photosensitizer (tested at 10 and 20 µM in vitro and at 20 and 50 µM) and dental curing light as light source. Results: In in vitro studies, we have observed viability decrease on C. albicans fungi and E. faecalis bacteria, above 3 log UFC/mL and 6 log UFC/mL, respectively, when exposed with a ProtoIX concentration of 20 µM and 20 minutes of dental curing light irradiation. In ex vivo studies, we have observed a slight decrease of C. albicans fungi survival (0.8 log UFC/mL), after its incubation on 20 µM of ProtoIX and 20 minutes irradiation, with a dental curing light. Conclusion: This study has shown aPDT’s high potential of periodontopathogens inactivation using ProtoIX as photosensitizer and dental curing light as light source. However, several more studies must be conducted to improve this protocol, so it can be used in clinical environments.
Description
Keywords
Periodontite aPDT ProtoIX Fotopolimerizador Periodontitis Dental curing light