| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 6.48 MB | Adobe PDF |
Authors
Advisor(s)
Abstract(s)
Introdução: A periodontite crónica avançada tem características muito
específicas, sendo a evolução desta dependente do estado de saúde oral e
periodontal do paciente, resultantes de fatores biológicos, hábitos de higiene e
saúde, genética e idade dos indivíduos. No entanto, o fator de maior importância
para a evolução da patologia periodontal é a presença de periodonto-patógenos
responsáveis por se aproveitarem do estado periodontal fragilizado.
O objetivo deste estudo foi a caracterização do biofilme periodonto-patogénico
numa amostra da população de pacientes com periodontite crónica avançada da
UCP através da avaliação da saúde oral e periodontal e da deteção molecular de
periodonto-patógenos específicos da PCA na saliva e no biofilme.
Materiais e Métodos: Uma amostra de 29 indivíduos foi estudada, constituindose
o grupo de controlo com 10 pacientes totalmente saudáveis e o grupo teste
com 19 pacientes com periodontite crónica avançada. Foram adotadas 3
estratégias para recolha de dados: questionário para recolha de dados
sociodemográficos, avaliação clínica da saúde oral e periodontal e recolha de
saliva e biofilme dentário.
Resultados: O estado saudável do grupo controlo foi confirmado tendo em conta
a ausência de doença periodontal, sendo que estes apenas estão presentes em
pacientes do grupo teste. O índice médio do CPO-d total do grupo controlo foi de
7 ± 4,137, ao contrário do grupo teste onde o índice médio do CPO-d total de
15,11 ± 9,11. Porphyromonas gingvalis e Filifactor alocis foram detetados no
biofilme de pacientes com PCA sendo possível realizar uma confirmação não
clínica da patologia nestas amostras.
Conclusão: No caso dos indivíduos com PCA, é observada a presença de
doença periodontal instalada, através da presença de bactérias específicas desta
periodontite no biofilme das bolsas periodontais do grupo teste, fazendo com que
a recolha e análise de biofilme seja de maior confiança.
Introduction: The evolution of severe chronic periodontitis is reliant on the patients oral and periodontal health status due to resulting biological factors, hygiene and health related habits, genetical determinants and patients age. Despite this, the major factors that most influence the evolution of the periodontal disease are the specific periodontal pathogens responsible for taking advantage of a debilitated periodontal status that enhances periodontal disease progression. The main objective was to study the periodontal pathogenic biofilm profile in individuals with severe chronic periodontitis disease from the dental clinic of UCP by evaluating oral and periodontal status and by molecular detection of periodontal pathogens in salva and biofilm samples of healthy and periodontal patients. Material and Methods: A sample of 29 patients was studied resulting in a control group with 10 completely healthy patients and a test group with 19 patients with severe chronic periodontitis. Three different data collection strategies were employed: sociodemographic data collection survey, clinical evaluation of oral and periodontal status and saliva and biofilm sample collection. Results: The healthy status of the control group was confirmed and no periodontal disease was reported. The same could not be said about the test group where severe chronic periodontitis was present on all samples. The DMFT index average score for the control group was 7 ± 4,137 contrasting with the test group’s average DMFT index of 15,11 ± 9,11. Porphyromonas gingvalis and Filifactor alocis were only detected on the dental biofilm of periodontal sockets of patients with severe chronic periodontitis making it possible to get a non-clinical confirmation of it. Conclusion: It is possible to observe the installed periodontal disease in patients with severe chronic periodontitis through the presence of specific periodontal bacteria in the biofilm of periodontal sockets of the test group, making the biofilm sample and analysis more reliable.
Introduction: The evolution of severe chronic periodontitis is reliant on the patients oral and periodontal health status due to resulting biological factors, hygiene and health related habits, genetical determinants and patients age. Despite this, the major factors that most influence the evolution of the periodontal disease are the specific periodontal pathogens responsible for taking advantage of a debilitated periodontal status that enhances periodontal disease progression. The main objective was to study the periodontal pathogenic biofilm profile in individuals with severe chronic periodontitis disease from the dental clinic of UCP by evaluating oral and periodontal status and by molecular detection of periodontal pathogens in salva and biofilm samples of healthy and periodontal patients. Material and Methods: A sample of 29 patients was studied resulting in a control group with 10 completely healthy patients and a test group with 19 patients with severe chronic periodontitis. Three different data collection strategies were employed: sociodemographic data collection survey, clinical evaluation of oral and periodontal status and saliva and biofilm sample collection. Results: The healthy status of the control group was confirmed and no periodontal disease was reported. The same could not be said about the test group where severe chronic periodontitis was present on all samples. The DMFT index average score for the control group was 7 ± 4,137 contrasting with the test group’s average DMFT index of 15,11 ± 9,11. Porphyromonas gingvalis and Filifactor alocis were only detected on the dental biofilm of periodontal sockets of patients with severe chronic periodontitis making it possible to get a non-clinical confirmation of it. Conclusion: It is possible to observe the installed periodontal disease in patients with severe chronic periodontitis through the presence of specific periodontal bacteria in the biofilm of periodontal sockets of the test group, making the biofilm sample and analysis more reliable.
Description
Keywords
Saliva Saúde oral Periodontite crónica avançada Biofilme dentário Periodonto-patógenos Saliva Oral health Severe chronic periodontitis Dental biofilm Periodontal pathogens