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- Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatmentPublication . Calheiros, Cristina S. C.; Pereira, Sofia I. A.; Franco, Albina R.; Castro, Paula M. L.Constructed wetlands (CWs) are biological wastewater treatment systems that comprise several components where plants and associated organisms play an important role in water depuration. Microbial studies emphasize bacterial dynamics, whereas studies of arbuscular mycorrhizal fungi (AMF) are scarce and the functional role of AMF in aquatic and wetland plants is poorly understood. The aim of this study was to analyze the AMF communities colonizing the roots of Canna indica, Canna flaccida, and Watsonia borbonica inhabiting a CW treating wastewater of a tourism unit. The dynamics of the AMF communities were evaluated by Denaturing Gradient Gel Electrophoresis (DGGE) of 18S rRNA gene amplification products along cold (C) and hot (H) seasons for three consecutive years. DGGE profiles allowed the estimation of AMF species richness (S), and Shannon-Wienner (H) and Pielou (J) indexes, for the different plant species, showing differences between species and along the years. Excised bands from DGGE were analyzed and identified through sequencing for arbuscular mycorrhiza, revealing the presence of AMF strains closely related to Glomus sp., Rhizophagus sp. and Acaulospora sp. genera. Concomitant water quality analyses showed that the system was effective in organic and nutrient removal during the sampling period. Findings from this study suggest that AMF diversity found in the CW is influenced by the water constituents, season, and plant species.
- Constructed Wetlands for freshwater and saline aquaculture wastewater treatment: a microcosm experiencePublication . Jesus, J. M.; Borges, M. T.; Calheiros, Cristina S. C.; Castro, Paula M. L.The aquaculture industry discharges large volumes of nutrient rich wastewater, contributing to eutrophication events. Recent culture intensification methodologies such as recirculation (RAS) and shallow raceway (SRS) systems discharge wastewater with even higher nutrient concentrations, though at lower volumes (Rana et al., 2005). Hence, efluent treatment options are of vital importance. Constructed wetlands (CWs) are a possible but underexplored treatment solution even for high salinity situations (Lymbery et al., 2006) consisting of planted shallow channels, relying upon biological, physical and chemical processes to treat wastewater (EPA, 2000). Therefore, this study aims to extend the knowledge on the possible use of CWs for aquaculture wastewater treatment, either fresh or saline. To fulfill this goal, several microcosms were created simulating subsurface systems with HRT = 7 days and planted with Typha latifolia. Substrate used was expanded clay 8 – 12.5 mm Ø. Macrophyte survival, growth, and nutrient removal (phosphate, nitrite, nitrate and ammonia) were assessed over a period of 4 weeks. Freshwater (simulated) and saline (real) fish farm effluents (recirculation SRS, 2.4% salinity) were tested. Results showed that plants adapted better to freshwater conditions exhibiting higher growth rate. Plants at 2.4% salinity did not growth in height after 2 weeks, but survival remained high.The microcosm wetland system was able to treat the effluent by removing 61%, 78% and 98% of NH4, NO2 and PO4, respectively, in fresh water, and 94%; 78%, 34% and 100% of NH4, NO2, NO3 and PO4, respectively, in saline wastewater. An improvement of removal with time was observed, suggesting the existence of a system adaptation period. The microcosm treatment was able to reduce nutrient concentrations to legally acceptable values. Hence, constructed wetlands can be an adequate solution for aquaculture efffluent treatment. Further studies are necessary, however, to achieve a better adaptation of the plant used to salinity.
- Evaluation of different substrates to support the growth of Typha latifolia in constructed wetlands treating tannery wastewater over long-term operationPublication . Calheiros, Cristina S. C.; Rangel, António O. S. S.; Castro, Paula M. L.The aim of this study was to investigate the performance of horizontal subsurface flow constructed wetlands planted with Typha latifolia treating tannery wastewater under long-term operation. Two expanded clay aggregates (Filtralite® MR3–8-FMR and Filtralite® NR3–8-FNR) and a fine gravel-FG were used as substrate for the constructed wetland units plus one unit with FMR was left as an unvegetated control. The systems were subject to three hydraulic loadings, 18,8 and 6cm d⁻¹, and to periods of interruption in the feed. The relationship between the substrate, plant development and removal efficiency, especially of organic matter, was investigated. Organic loadings up to 1800 kg BOD5 ha⁻¹ d⁻¹ and 3849 kg COD ha⁻¹ d⁻¹ were applied leading to mass removals of up to 652 kg BOD₅ ha⁻¹ d⁻¹ and 1869 kg COD ha⁻¹ d⁻¹, respectively. The three different substrates were adequate for the establishment of T. latifolia, although the clay aggregates allowed for higher plant propagation levels. The units with FNR and FMR achieved significantly higher COD and BOD₅ removal when compared to the FG and to the unplanted units. The systems proved to be tolerant to high organic loadings and to interruptions in feed suggesting this technology as a viable option for the biological treatment of tannery wastewater.
- Constructed wetlands for tannery wastewater treatment in Portugal: ten years of experiencePublication . Calheiros, Cristina S. C.; Rangel, António O. S. S.; Castro, Paula M. L.Wastewaters from tannery industry are complex in composition and providing adequate treatment can be difficult. Constructed wetlands (CW) are regarded as an alternative treatment to the conventional biological systems, as a developing cost-effective and environmentally friendly phytoremediation technology. The present review compiles and integrates information on CWs technology for the needs of the tannery sector. The following issues arise as crucial for the implementation of such systems, namely i) an accurate wastewater characterization and an effective pretreatment before reaching the CW, ii) choosing the plants species better adapted to the imposed conditions, iii) substrate selection and iv) range of organic loadings applied. The examples practiced in Portugal give indication that horizontal subsurface flow systems, with expanded clay media, are a suitable option to be considered when dealing with high organic loading tannery wastewater (up to c.a. 3800 kgCODha−1d−1), being resilient to a wide range of hydraulic variations. Plants such as Phragmites and Typha have shown to be adequate for tannery wastewater depuration, with Arundo donax proving resilient to high salinity wastewaters. The flexibility of implementation allows the CW to be adapted to different sites with different configurations, being suitable as main secondary or tertiary treatment stage.
- Biological processes for industrial wastewater treatment- case study in the leather industryPublication . Calheiros, Cristina S. C.; Rangel, António O. S. S.; Castro, Paula M. L.
- Constructed wetland systems vegetated with different plants applied to the treatment of tannery wastewaterPublication . Calheiros, Cristina S.C.; Rangel, António O.S.S.; Castro, Paula M.L.Wastewaters from leather processing are very complex and lead to water pollution if discharged untreated, especially due to its high organic loading. In this study the survival of different plant species in subsurface horizontal flow constructed wetlands receiving tannery wastewater was investigated. Five pilot units were vegetated with Canna indica, Typha latifolia, Phragmites australis, Stenotaphrum secundatum and Iris pseudacorus, and a sixth unit was left as an unvegetated control. The treatment performance of the systems under two different hydraulic loading rates, 3 and 6 cmd1, was assessed. COD was reduced by 41–73% for an inlet organic loading varying between 332 and 1602 kg ha1 d1 and BOD5 was reduced by 41–58% for an inlet organic loading varying between 218 and 780 kg ha 1 d1. Nutrient removal occurred to lower extents. Phragmites australis and Typha latifolia were the only plants that were able to establish successfully. Despite the high removal of organic content from the influent wastewater, during 17 months of operation, no significant differences in performance were observed between units.
- Constructed wetland with a polyculture of ornamental plants for wastewater treatment at a rural tourism facilityPublication . Calheiros, Cristina S. C.; Bessa, Vânia S.; Mesquita, Raquel B. R.; Brix, Hans; Rangel, António O. S. S.; Castro, Paula M. L.Sewage management in remote rural and mountain areas constitutes a challenge because of the lack of adequate infrastructure and economic capability. Tourism facilities, in particular, possess a special challenge because of huge variability in sewage production and composition as a consequence of variations in number of guests and their activities. Constructed wetlands (CWs) are recognized as a robust and economical ecotechnology capable of meeting these challenges. A horizontal subsurface flow CW system was established at a guest house located in a rural and mountain area of Portugal. The substrate of the bed was an expanded clay substrate, and the system was planted with a polyculture of ornamental flowering plants (Canna flaccida, Zantedeschia aethiopica, Canna indica, Agapanthus africanus and Watsonia borbonica). The load and composition of sewage varied significantly seasonally (17–579 kg COD ha−1 d−1), but removal efficiencies of BOD and COD were generally high (>90%) and independent of the loading conditions. The system also reduced PO43− (up to 92%), NH4+ (up to 84%) and total coliform bacteria (up to 99%). The ornamental polyculture provided an aesthetic pleasing system with different appearance during the seasons. Of the five species tested, four grew well (C. flaccida, C. indica, Z. aethiopica and W. borbonica), whereas A. africanus was outcompeted. The system owner cut flowers from the CW system and used them for decorations at the guest house. It was demonstrated that CW systems planted with a polyculture of ornamental plant species, besides the water treatment function, possess several additional benefits including aesthetics and biodiversity enhancement.
- Toxicity abatement of wastewaters from tourism units by constructed wetlandsPublication . Calheiros, Cristina S. C.; Castro, Paula M. L.; Gavina, Ana; Pereira, RuthThe present research intended to investigate the toxicity abatement of domestic wastewater after passing a biosystem composed of a constructed wetland (CW) followed by a pond. The wastewater was generated in a tourism house in a rural and mountainous context and passed through a septic tank before being diverted to a CW followed by a pond. A battery of ecotoxicological tests, comprising microalgae (Raphidocelis subcapitata), macrophytes (Lemna minor), cladocerans (Daphnia magna), and bacteria (Aliivibrio fischeri), was used to assess the toxicity of the wastewater collected before and after the CW and the water of the pond. Physicochemical parameters (pH, conductivity, chemical oxygen demand, biochemical oxygen demand, total suspended solids, phosphates, ammonium, and nitrate) were also determined. The CW was able to remove carbon and nutrients from the water with a concomitant reduction of its toxicity. This study, reinforced the added value of using toxicity tests as a complement to CW operational monitoring to validate the solution and to analyze possible readjustments that may be required to improve efficiency. This study lends further support to the claim that CWs can be a sustainable solution for treating small volumes of domestic wastewater in a rural context.
- Spatial-temporal changes in removal of fecal indicators and diversity of bacterial communities in a constructed wetland with ornamental plantsPublication . Calheiros, Cristina S. C.; Pereira, Sofia I. A.; Franco, Albina R.; Castro, Paula M. L.The present study was undertaken in a constructed wetland (CW), setup in a tourism house, for domestic wastewater treatment. The influence of season variations on the abundance of fecal indicator organisms (total coliforms and Escherichia coli) in the wastewater and in the substrate and the roots of plants inhabiting the inlet and outlet zones of the CW was evaluated along three consecutive years. The structure and diversity of bacterial communities associated to the CW’s substrate of inlet and outlet zones was also analyzed overtime. Wastewater was characterized for physicochemical and microbiological parameters and the bacterial communities colonizing the substrate surface, were analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). The CW was effective in removing COD, BOD5, TSS, PO43−, NH4+, NO3−, and NO2− . It was also effective in removing fecal indicators, with a generalized decrease of total coliforms and E. coli in the substrate and in the wastewater from inlet to outlet of up to 2–3 log. The structure and composition of bacterial communities associated with the substrate was mainly influenced by the year rather than by the season or the CW zone.