Percorrer por autor "Santos, Carla S."
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- Challenges in pea breeding for tolerance to drought: status and prospectsPublication . Bagheri, Maryam; Santos, Carla S.; Rubiales, Diego; Vasconcelos, Marta W.Drought is increasingly frequent in the context of climate change and is considered a major constraint for crop yield. Water scarcity can impair growth, disturb plant water relations, and reduce water use efficiency. Pea (Pisum sativum) is a temperate grain legume rich in protein, fiber, micronutrients, and bioactive compounds that can benefit human health. In reducing pea yield due to drought, the intensity and duration of stress are critical. This review describes several drought resistance mechanisms in pea based on morphology, physiology, and biochemical changes during/after the water deficit period. Drought tolerance of pea can be managed by adopting strategies such as screening, breeding, and marker-assisted selection. Therefore, various biotechnological approaches have led to the development of drought-tolerant pea cultivars. Finally, the main objective of the current research is to point out some useful traits for drought tolerance in peas and also, mention the methods that can be useful for future studies and breeding programmes.
- Chitosan increases Pinus pinaster tolerance to the pinewood nematode (Bursaphelenchus xylophilus) by promoting plant antioxidative metabolismPublication . Silva, Marta Nunes da; Santos, Carla S.; Cruz, Ana; López-Villamor, Adrián; Vasconcelos, Marta W.The pine wilt disease (PWD), for which no effective treatment is available at the moment, is a constant threat to Pinus spp. plantations worldwide, being responsible for significant economic and environmental losses every year. It has been demonstrated that elicitation with chitosan increases plant tolerance to the pinewood nematode (PWN) Bursaphelenchus xylophilus, the causal agent of the PWD, but the biochemical and genetic aspects underlying this response have not been explored. To understand the influence of chitosan in Pinus pinaster tolerance against PWN, a low-molecular-weight (327 kDa) chitosan was applied to mock- and PWN-inoculated plants. Nematode population, malondialdehyde (MDA), catalase, carotenoids, anthocyanins, phenolic compounds, lignin and gene expression related to oxidative stress (thioredoxin 1, TRX) and plant defence (defensin, DEF, and a-farnesene synthase, AFS), were analysed at 1, 7, 14, 21 and 28 days post-inoculation (dpi). At 28 dpi, PWN-infected plants elicited with chitosan showed a sixfold lower nematode population when compared to non-elicited plants. Higher levels of MDA, catalase, carotenoids, anthocyanins, phenolic compounds, and lignin were detected in chitosan-elicited plants following infection. The expression levels of DEF gene were higher in elicited plants, while TRX and AFS expression was lower, possibly due to the disease containment-effect of chitosan. Combined, we conclude that chitosan induces pine defences against PWD via modulation of metabolic and transcriptomic mechanisms related with plant antioxidant system.
- Combined ‘omics’ approaches to dissect the root´s iron uptake system in soybean plantsPublication . Vasconcelos, Marta W.; Santos, Carla S.; Machado, Adelaide; Pinheiro, Carla; Ricardo, Cândido Pinto
- A combined physiological and biophysical approach to understand the ligand-dependent efficiency of 3-hydroxy-4-pyridinone Fe-chelatesPublication . Santos, Carla S.; Leite, Andreia; Vinhas, Sílvia; Ferreira, Sofia; Moniz, Tânia; Vasconcelos, Marta W.; Rangel, Maria da ConceiçãoLigands of the 3‐hydroxy‐4‐pyridinone (3,4‐HPO) class were considered eligible to formulate new Fe fertilizers for Iron Deficiency Chlorosis (IDC). Soybean (Glycine max L.) plants grown in hydroponic conditions and supplemented with Fe‐chelate [Fe(mpp)3] were significantly greener, had increased biomass, and were able to translocate more iron from the roots to the shoots than those supplemented with an equal amount of the commercially available chelate [FeEDDHA]. To understand the influence of the structure of 3,4‐HPO ligand on the role of the Fe‐chelate to improve Fe‐uptake, we investigated and report here the effect of Fe‐chelates ([Fe(mpp)3], [Fe(dmpp)3], and [Fe(etpp)3]) in addressing IDC. Chlorosis development was assessed by measurement of morphological parameters, quantification of chlorophyll and Fe, and other micronutrient contents, as well as measurement of enzymatic activity (FCR) and gene expression (FRO2, IRT1, and Ferritin). All [Fe(3,4‐HPO)3] chelates were able to provide Fe to plants and prevent IDC but with a different efficiency depending on the ligand. We hypothesize that this may be related with the distinct physicochemical characteristics of ligands and complexes, namely, the diverse hydrophilic–lipophilic balance of the three chelates. To test the hypothesis, we performed an EPR biophysical study using liposomes prepared from a soybean (Glycine3 max L.) lipid extract and spin probes. The results showed that the most effective chelate [Fe(mpp)3] shows a preferential location close to the surface while the others prefer the hydrophobic region inside the bilayer.
- Comparative analysis of Iron Deficiency Chlorosis responses in soybean (Glycine max) and barrel medic (Medicago truncatula)Publication . Santos, Carla S.; Serrão, Inês; Vasconcelos, Marta W.Legume grains have an important socio-economical role, being highly utilized in human and animal nutrition. Although iron (Fe) is abundant in the earths crust, its limited solubility makes it poorly bioavailable for plants, contributing to iron deficiency chlorosis (IDC). In this work the physiological and molecular mechanisms associated with IDC were studied, namely, the mechanisms involved on Fe deficiency response, as well as a new Fe metabolism related gene in two important legume crops, Glycine max and Medicago truncatula. Fe deficient plants developed: decreased root and shoot length, increased number of secondary roots and lower chlorophyll levels. Fe shoot content decreased six- and 11-fold for G. max and M truncatula in Fe-deficiency. Whilst in G. max roots no significant differences were detected, in M. truncatula roots Fe decreased nine-fold in Fe-deficiency. Genes involved in Fe uptake (FRO2-like and IRT1-like), were over-expressed in roots of Fe-sufficient G. max and in Fe-deficient M. truncatula. VIT1-like, YSL1-like and ferritin presented higher expression levels in Fe-sufficient shoots and roots, whereas NRAMP3-like and GCN2-like showed higher expression values in Fe-deficiency.
- Correction to: resistance of Pinus pinea to Bursaphelenchus xylophilus explained by the dynamic response of phytohormones, antioxidant activity, and stress-related gene expression (Trees, (2025), 39, 1, (21), 10.1007/s00468-024-02594-7)Publication . Silva, Marta Nunes da; Santos, Carla S.; Solla, Alejandro; Gamir, Jordi; Flors, Victor; Sampedro, Luis; Zas, Rafael; Vasconcelos, Marta W.The corresponding author name has been incorrectly swapped in the original publication. The complete correct name should read as follows. Marta Nunes da Silva In addition, the funding information has been revised. The complete correct funding information is given below. The study was performed within the ‘Characterization of the pinewood nematode/Pinus system: a phytochemical and histopatho-logical approach’ project (PTDC/AGR-CFL/120184/2010), funded by Fundação para a Ciência e a Tecnologia (FCT). Additional financial support came from the Spanish National Research Agency FENOPIN (AGL2012-40151), RESILPINE (RTI2018-094691-B-C33) grants, Intramural-201640I030, OTR07700, IN607A2021/03, and from the FCT’s bilateral action Spain-Portugal PRIAIBPT-2011-1152 (NEMARES), UIDB/50016/2020 R&D Unit, and 2022.01903.CEECIND and 2023.06124.CEECIND programs. The original article has been corrected.
- Development of a healthy lentil-based food productPublication . Geraldo, Rafaela A. F.; Santos, Carla S.; Silva, Marta Nunes da; Pinto, Elisabete; Vasconcelos, Marta W.
- Development, processing, and acceptance of a faba bean (vicia faba var. Minor) based porridgePublication . Perez, Jazmín Ososrio; Santos, Carla S.; Vasconcelos, Marta
- Development, processing, and acceptance of a faba bean (Vicia faba) based porridgePublication . Perez, Jazmín Osorio; Santos, Carla S.; Vasconcelos, Marta W.The development of legume-based food products allows the promotion of healthy and nutritious plant-based alternatives, which are also environmentally sustainable. Porridges are often healthy, simple to prepare and suitable for various segments of the population, constituting a good option for food innovation. In this study a faba bean-based porridge was developed, using freeze-dried faba bean flour. A randomised sensorial analysis was performed. In terms of colour, 43% of panellists indicated that they “like it to some extent”, while the texture was the most appreciated characteristic which was evaluated. Results showed that overall appreciation of the porridge was 40%.
- Differential responses of Kabuli and Desi chickpeas (Cicer arietinum) to low water provision and their mineral profilingPublication . Medeiros, Janaína; Silva, Marta Nunes da; Santos, Carla S.; Vasconcelos, Marta W.Legume grains are of great importance for agriculture and the environment due to their ability to symbiotically fix atmospheric nitrogen and provide protein, minerals, vitamins, and other bioactive nutrients (Geraldo et al., 2022). Chickpea (Cicer arietinum) is one of the most consumed legumes worldwide and it has gained even more importance in recent decades. Production levels have increased, their incorporation as intercrops has been promoted, and they have been used in the formulation of novel food products (Saget et al., 2020). Nonetheless, the exploitation of traditional chickpea varieties, such as the Desi type (black coloured chickpea), has been overlooked, and the recovery of under-exploited traditional varieties could contribute to fostering biodiversity, and promoting environmental sustainability and diversifying diets. However, current knowledge on the nutritional profile of commercial and traditional chickpea varieties and their resilience degree to environmental stresses, such as water scarcity, is limited, thus being the focus of this work.