Browsing by Author "Michel, Mariela R."
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- Enhancing the insecticidal efficacy of Allium sativum extracts through microencapsulation via complex coacervationPublication . Michel, Mariela R.; Aguilar-Zárate, Mayra; Perales-Rosas, Daniel; Martínez-Ávila, Guillermo Cristian G.; Gómez-García, Ricardo; Tafolla-Arellano, Julio C.; Rojas, Romeo; Aguilar-Zárate, PedroGarlic (Allium sativum L.) has been widely studied for its insecticidal properties. The primary bioactive molecule in garlic extracts include allicin, alliin, S-allylcysteine, diallyl disulfide, diallyl trisulfide, diallyl sulfide and ajoene. However, these compounds degrade under environmental conditions once extracted. This study aimed to enhance the effectiveness of garlic extracts in controlling Tenebrio molitor by optimizing microencapsulation techniques. The garlic extracts were encapsulated using the complex coacervation method, with independent variables including pH levels (3, 6 and 9), whey protein isolate (WPI) (4 %, 6 % and 8 % w/v) and pectin (0.50 %, 0.75 % and 1.00 % w/v). A Taguchi L9 (33) orthogonal array was employed to design 9 treatments and T. molitor mortality was assessed 72 h after a 10 sec immersion of the insects in the treatments. Statistical analysis revealed that WPI had the most significant influence (24.52 %), followed by pH (18.82 %) and pectin (7.79 %). The interaction between pH and pectin had the greatest effect on the encapsulation process, accounting for 38.65 % of the influence. The optimal microencapsulation conditions were predicted by software to be pH 3, a pectin concentration of 0.75 % w/v and a WPI concentration of 4.00 % w/v, resulting in a signal-to-noise (S/N) ratio of 42.30. Experimental validation of these conditions produced an S/N ratio of 18.54, corresponding to a T. molitor mortality rate of 92 ± 4.47 %. The resulting microcapsules had diameters ranging from 1-5 μm. Complex coacervation is a highly promising method for microencapsulating garlic extracts and preserving their insecticidal properties.
- Grape pomace - advances in its bioactivity, health benefits, and food applicationsPublication . Almanza-Oliveros, Angélica; Bautista-Hernández, Israel; Castro-López, Cecilia; Aguilar-Zárate, Pedro; Meza-Carranco, Zahidd; Rojas, Romeo; Michel, Mariela R.; Martínez-Ávila, Guillermo Cristian G.From a circular economy perspective, the appropriate management and valorization of winery wastes and by-products are crucial for sustainable development. Nowadays, grape pomace (GP) has attracted increasing interest within the food field due to its valuable content, comprising nutritional and bioactive compounds (e.g., polyphenols, organic and fatty acids, vitamins, etc.). Particularly, GP polyphenols have been recognized as exhibiting technological and health-promoting effects in different food and biological systems. Hence, GP valorization is a step toward offering new functional foods and contributing to solving waste management problems in the wine industry. On this basis, the use of GP as a food additive/ingredient in the development of novel products with technological and functional advantages has recently been proposed. In this review, we summarize the current knowledge on the bioactivity and health-promoting effects of polyphenolic-rich extracts from GP samples. Advances in GP incorporation into food formulations (enhancement of physicochemical, sensory, and nutritional quality) and information supporting the intellectual property related to GP potential applications in the food industry are also discussed.
- Location and tissue effect on physicochemical, mineral and amino acid profile of Moringa oleiferaPublication . Gómez-Martínez, Martha; Garcia-Ortiz, Jesús David; Gómez-Martínez, Susana; Michel, Mariela R.; López-Badillo, Claudia M.; Pintado, Manuela; Rodríguez-Herrera, RaúlIntroduction: Moringa oleifera is a medicinal plant with multiple biological uses, whose tissues have not been studied individually. The objective of this work was to evaluate the location effect on physicochemical, techno-functional, mineral and amino acid profile of two Moringa tissues (leaflets and petioles). Methods: Two Moringa tissues grown in three different locations were characterized physicochemically (fat, fiber, moisture, protein, carbohydrates), techno-functionally (water-holding capacity, swelling capacity, oil-holding capacity and organic molecule absorption capacity), mineral (by fluorecancer x-rays) and aminoacid (by HPLC). Results: Results indicated that leaflets showed higher dry matter content (89.40 ± 0.86 %), ash (11.66 ± 0.59 %), protein (34.61 ± 3.75 %), fat (15.01 ± 1.51 %), water-holding capacity (5.03 mL/g), and swelling capacity (7.28 cm3/g) compared to petioles which presented higher fiber content (36. 21 ± 3.75 %), oil-holding capacity (2.67 g oil/g), and organic molecule absorption capacity (2.91 mL oil/g). In addition, leaflets showed a higher mineral content in comparison to petioles, in both tissues, the major elements were Ca, K, Mg and S. On the other hand, leaflets showed a higher content and diversity of essential and nonessential amino acids. Conclusion: In conclusion, quality and physicochemical, techno-functional, elemental and amino-acid profile are affected by tissue and location.
- The specific encapsulation of procyanidins from litchi peel and coffee pulp extracts via spray-drying using green polymersPublication . Vázquez-Núñez, María de los Ángeles; Aguilar-Zárate, Mayra; Gómez-García, Ricardo; Reyes-Luna, Carlos; Aguilar-Zárate, Pedro; Michel, Mariela R.Polyphenols called procyanidins can be extracted from agro-industrial waste like litchi peel and coffee pulp. However, their efficacy is limited due to instability, which hinders both the bioavailability and preservation of their activity. This study aims to establish the ideal encapsulation conditions required to preserve the procyanidin properties found in extracts taken from litchi peel and coffee pulp. To attain the maximum procyanidin encapsulation efficacy (EE), the Taguchi method was utilized to streamline the spray-drying conditions for different wall materials—maltodextrin (MD), whey protein (WP), citrus pectin (CP), and skim milk (SM). The optimized conditions consisted of feed flow (3, 4.5, and 6 mL/min), temperature (125, 150, and 175 °C), and airflow (30, 35, and 40 m3/h). The microcapsules were characterized using ABTS, DPPH, lipoperoxidation, and scanning electron microscopy. Objective evaluations revealed that MD was the most effective encapsulation material for the litchi extract, whereas WP was the optimal option for the coffee extract. Of all the factors considered in the spray-drying process, feed flow had the strongest impact. The spray-drying process for the litchi peel extracts achieved high procyanidin encapsulation efficiencies at a feed flow rate of 4.5 mL/min, a temperature of 150 °C, and an airflow rate of 35 m3/h. Meanwhile, the coffee extract spray drying achieved similar results at a feed flow rate of 4.5 mL/min, a temperature of 175 °C, and an airflow rate of 40 m3/h. Encapsulation efficiencies of 98.1% and 93.6% were observed for the litchi and coffee extracts, respectively, under the mentioned optimal conditions. The microencapsulation process was successful in preserving the antioxidant properties of procyanidins. The microcapsules’ size ranged from 2.6 to 3.2 micrometers. The results imply that the phenolic compounds present in the extracts function as effective antioxidant agents.