Browsing by Author "Machado, D."
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- Akkermansia muciniphila antimicrobial susceptibility profilePublication . Barbosa, J. C.; Machado, D.; Almeida, D.; Andrade, J. C.; Freitas, A. C.; Gomes, A. M.
- Analyzing the potential of selected gut commensal strains to produce antimicrobial peptides: phenotypic and in silico approachesPublication . Fonseca, M.; Machado, D.; Vedor, R.; Andrade, J. C.; Gomes, A. M.; Barbosa, J. C.The World Health Organization estimates that the number of antibiotic resistance-related deaths could reach 10 million by 2050 [1]. Given the dynamics and high diversity of microorganisms that inhabit the gastrointestinal tract, this is the ideal place to discover new antimicrobial peptides (AMP) to replace traditional antibiotics [2]. Among the most extensively studied members are the commensal strains Akkermansia muciniphila DSM 22959 and Faecalibacterium duncaniae DSM 17677, which are reported to have a beneficial impact on intestinal health [3;4]. Antimicrobial peptides are small molecules that act as the first line of defense against microbial invaders, playing a vital role in the innate immune system [5]. One approach to identify new strategies to combat antimicrobial resistance is to evaluate the ability of these bacteria to produce AMP.
- Characterization of 70.5% cocoa content dark chocolate incorporated with Bifidobacterium animalis subspecies lactis BB-12®Publication . Vedor, R.; Machado, D.; Barbosa, J. C.; Gomes, A. M.Currently, probiotics are defined as "live microorganisms that, when administered in adequate amounts, confer a health benefit on the host” [1].Bifidobacterium animalis subsp. lactis BB-12® is one of the most common probiotics used as a food supplement [2]. Chocolate is one of the most popular and appealing culinary products, with various cocoa percentages available to consumers [3;4]. Several studies have suggested that chocolate, particularly dark chocolate, may offer health benefits due to the presence of a diverse array of bioactive compounds [5]. In this sense, a growing interest in using chocolate as a carrier for probiotic delivery has emerged.
- Formulation strategies for enhancing growth of akkermansia muciniphila and its survival through lyophilisation and storage at air ambientPublication . Freitas, A.; Almeida, D.; Machado, D.; Mendo, S.; Gomes, A.Gut bacterium Akkermansia muciniphila has been recognized as a new potential probiotic due to promising outcomes in the prevention and treatment of several human diseases. However, despite its multiple beneficial effects, its viability is compromised by the presence of oxygen. This has so far precluded its application namely in the development of nutraceutical/therapeutic formulations. In order to enhance growth of A. muciniphila and its survival through lyophilisation and storage at air ambient, formulation strategies are presented based on use of prebiotic compounds, antioxidants and cryoprotectants. According to our best knowledge, there is no information regarding lyophilisation procedures involving A. muciniphila as well as its subsequent storage at ambient air.
- Hydrophobicity and aggregation properties of gut commensals faecalibacterium duncaniae DSM 17677 and akkermansia muciniphila DSM 22959Publication . Fonseca, M.; Machado, D.; Vedor, R.; Barbosa, J. C.; Andrade, J. C.; Gomes, A. M.Probiotics have been emerging as a promising approach to prevent and control foodborne diseases [1]. In the last years, the bacterial species isolated from gut microbiota, such as Faecalibacterium spp. and Akkermansia muciniphila, have been proposed as novel probiotic candidates [2]. The cell surface hydrophobicity, auto-aggregation and co-aggregation with pathogens are considered desirable characteristics of probiotic strains and these properties may be used in preliminary screening to identify potential probiotic microorganisms appropriate for human or animal use [3].
- Incorporation of Akkermansia muciniphila DSM 22959 in chocolate matricesPublication . Vedor, R.; Machado, D.; Barbosa, J. C.; Andrade, J. C.; Gomes, A. M.
- Investigating the susceptibility of the next generation probiotic faecalibacterium prausnitzii under stress conditionsPublication . Machado, D.; Domingos, M.; Almeida, D.; Barbosa, J. C.; Andrade, J. C.; Freitas, A. C.; Gomes, A. M.
- Potential of Akkermansia muciniphila DSM 22959 and Faecalibacterium duncaniae DSM 17677 as live biotherapeutics for intestinal infectionsPublication . Machado, D.; Vedor, R.; Fonseca, M.; Bento, M.; Barbosa, J. C.; Almeida, D.; Andrade, J. C.; Gomes, A. M.