Percorrer por autor "Leal, D."
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- Impact of feeding a new sustainable insect hydrolysate to adult Beagle dogs on diet digestibility and cardiac healthPublication . Leal, D.; Barroso, C.; Fontes-Sousa, A. P.; Borges, S.; Maia, M. R. G.; Almeida, A.; Pintado, M.; Fonseca, A. J. M.; Cabrita, A. R. J.Introduction: In recent years, there has been increasing attention on sustainable protein sources for both food and feed, leading to a growing interest in edible insects. However, the potential impact of their bioactive properties on health remains limited. This study aimed to address this gap by investigating the effects of dietary inclusion of Hermetia illucens (BSF) hydrolysates on diet palatability, nutrient digestibility, and cardiac health of adult Beagle dogs. Material and methods: Three complete commercial diets were formulated with the inclusion of 3% shrimp hydrolysate or 3% BSF hydrolysate obtained by enzymatic hydrolysis with Alcalase® 2.5 L or Corolase PP. Palatability was assessed in two-bowl tests with 12 dogs. The feeding trial followed a replicated Latin square design with 12 dogs, three periods of 28 days each, and three diets. Cardiac biomarkers and apparent total tract digestibility of nutrients and energy were evaluated at the end of each period. Results: The dietary inclusion of BSF hydrolysates was well accepted by dogs, with the BSF-Alcalase diet showing higher dry matter and protein digestibility (P < 0.05). No significant differences were noted in serum levels of C-reactive protein, cardiac troponin I, and pro B-type natriuretic peptide among diets and echocardiographic measurements were within the reference ranges. However, dogs on the BSF-Alcalase diet tended (P < 0.10) to increase serum angiotensin-converting enzyme activity and decrease serum insulin-like growth factor 1 levels, while arterial blood pressure tended to be lower in dogs on the BSF-Corolase diet. Conclusion: Dogs readily accepted diets supplemented with BSF hydrolysates, but the nutritional impact of insect hydrolysates varied depending on the enzymatic hydrolysis meth od employed. While BSF-Alcalase improved diet digestibility, BSF-Corolase may influence mechanisms regulating blood pressure.
- New ingredients with functional properties from hermetia illucens and tenebrio molitor through the enzymatic hydrolysis with corolasePublication . Leal, D.; Borges, S.; Almeida, A.; Maia, M. R. G.; Fonseca, A. J. M.; Cabrita, A. R. J.; Pintado, M.
- Protein hydrolysates from Tenebrio molitor and Hermetia illucens as novel food sources for dogsPublication . Leal, D.; Borges, S.; Almeida, A.; Pintado, M.; Fonseca, A. J. M.; Cabrita, A. R. J.There has been a growing interest in insect meals as a sustainable alternative protein source for animal food and feed. In parallel, insect hydrolysates have been investigated in vitro for their bioactive properties, but the impact of dietary inclusion on dog’s nutritional parameters needs to be elucidated. This study aimed to evaluate the chemical composition and bioactive properties of four insect hydrolysates obtained from the enzymatic hydrolysis of Tenebrio molitor and Hermetia illucens using ALCALASE 2.5L and Corolase PP enzymes and investigate the effects of their dietary inclusion on diet palatability, digestibility, and fecal characteristics and metabolites of adult Beagle dogs. Four two-bowl tests were performed using 12 adult Beagle dogs to assess palatability by the pairwise comparison between the control diet (a commercial diet with 3% of shrimp hydrolysate) and each of the four experimental diets (control diet with the replacement of 3% (w/w) of shrimp hydrolysate by 3% of each insect protein hydrolysate). A digestibility trial designed according to a replicated Latin square 5 × 5 design, with ten adult dogs, five periods of 10 d each, and five diets was performed to evaluate the effects on food intake, fecal characteristics and metabolites, apparent total tract digestibility, and estimated metabolizable energy content of the control and experimental diets. Chemical composition and in vitro antioxidant and antihypertensive activities of insect hydrolysates depended on the insect species and enzyme used. No differences were observed in the first diet approached or tasted, but the inclusion of hydrolysates of T. molitor obtained from Corolase PP hydrolysis showed a greater intake ratio (P = 0.032). Food intake, diet digestibility and fecal characteristics were not different between diets, except for an increased fecal caproate concentration in dogs fed the control diet (P = 0.024). The dietary inclusion of insect hydrolysates did not affect nutritional parameters, and further investigation is needed to evaluate their health-promoting properties for pet foods and supplements.