Browsing by Author "Freitas, Ana Cristina"
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- Aggregation properties of probiotic strains under aerobic and anaerobic conditionsPublication . Machado, Daniela; Andrade, José Carlos; Barbosa, Joana Cristina; Costa, Francisca; Fonseca, Mariana; Almeida, Diana; Freitas, Ana Cristina; Gomes, Ana Maria
- Aggregation properties of probiotic strains under aerobic and anaerobic conditionsPublication . Machado, Daniela; Andrade, José Carlos; Barbosa, Joana Cristina; Costa, Francisca; Fonseca, Mariana; Almeida, Diana; Freitas, Ana Cristina; Gomes, Ana Maria
- Akkermansia muciniphila antimicrobial susceptibility profilePublication . Barbosa, Joana Cristina; Machado, Daniela; Almeida, Diana; Andrade, José Carlos; Freitas, Ana Cristina; Gomes, Ana Maria
- Akkermansia muciniphila robustness towards different temperatures, atmospheres and gastrointestinal conditionsPublication . Freitas, Ana Cristina; Machado, Daniela; Almeida, Diana; Seabra, Catarina; Andrade, José Carlos; Gomes, Ana Maria
- Akkermansia muciniphila robustness towards different temperatures, atmospheres and gastrointestinal conditionsPublication . Freitas, Ana Cristina; Machado, Daniela; Almeida, Diana; Seabra, Catarina; Andrade, José Carlos; Gomes, Ana Maria
- Analytical approaches for proteomics and lipidomics of arsenic in algaePublication . Freitas, Ana Cristina; Gomes, Ana MariaArsenic (As) is an element with a complex chemistry found in relatively high concentrations in the marine environment. A diverse group of As compounds or As species exist in the marine environment and these are of importance to human health given their potential cytotoxicity. It has been pointed out that marine algae are the origin of such As compounds, since they accumulate arsenate from seawater and metabolize it into several organoarsenic species of both lipophilic and hydrophilic nature. In this chapter, it is intended to discuss lipidomics approaches for identification of the > 70 existing lipophilic arsenic species (arsenolipids) in algae which include arsenic incorporated into phospholipids, hydrocarbons, long-chain alcohols, and polyunsaturated fatty acids. In turn, proteomic analysis will also be presented and discussed in its role, for example, to investigate whether cells show a specific response or survival strategy when under arsenic-induced stress. Research on proteome involvement in stress and tolerance to arsenic offers new tools to understand the associated physiological and biochemical mechanisms connected with different algae responses. Protein biomarkers are also of interest to enable indication of quantitative changes in some physiological parameters as the result of stress or toxicity.
- Antimicrobial susceptibility profile of faecalibacterium prausnitzii DSM 17677 a novel probiotic candidatePublication . Machado, Daniela; Barbosa, Joana; Domingos, Melany; Almeida, Diana; Andrade, José Carlos; Gomes, Ana Maria; Freitas, Ana Cristina
- Bioconversion of fish discards through the production of lactic acid bacteria and metabolites: sustainable application of fish peptones in nutritive fermentation mediaPublication . Vázquez, José António; Durán, Ana I.; Menduíña, Araceli; Nogueira, Margarita; Gomes, Ana Maria; Antunes, Joana; Freitas, Ana Cristina; Dagá, Esther; Dagá, Paula; Valcarcel, JesusIn the current work, we study the capacity of 30 peptones obtained by enzyme proteolysis of ten discarded fish species (hake, megrim, red scorpionfish, pouting, mackerel, gurnard, blue whiting, Atlantic horse mackerel, grenadier, and boarfish) to support the growth and metabolite production of four lactic acid bacteria (LAB) of probiotic and technological importance. Batch fermentations of Lactobacillus plantarum, L. brevis, L. casei, and Leuconostoc mesenteroides in most of the media formulated with fish peptones (87% of the cases) led to similar growths (quantified as dry-weight biomass and viable cells) and metabolites (mainly lactic acid) than in commercial control broth (MRS). Comparisons among cultures were performed by means of the parameters obtained from the mathematical fittings of experimental kinetics to the logistic equation. Modelling among experimental and predicted data from each bioproduction was generally accurate. A simple economic assessment demonstrated the profitability achieved when MRS is substituted by media formulated with fish discards: a 3–4-fold reduction of costs for LAB biomass, viable cells formation, and lactic and acetic acid production. Thus, these fish peptones are promising alternatives to the expensive commercial peptones as well as a possible solution to valorize discarded fish biomasses and by-products.
- Characterization of a concentrated second cheese wheyPublication . Faustino, Margarida; Salsinha, Ana Sofia; Pimentel, Lígia; Alcalá, Luis; Freitas, Ana Cristina; Gomes, Ana Maria; Pereira, Carlos Dias; Pintado, Manuela
- Characterization of concentrated second cheese wheyPublication . Faustino, Margarida; Salsinha, Ana Sofia; Pimentel, Lígia; Alcalá, Luis; Freitas, Ana Cristina; Gomes, Ana Maria; Pereira, Carlos Dias; Pintado, ManuelaThe residual deproteinized whey known as second cheese whey or “Sorelho”, is a byproduct obtained from whey cheese manufacture. It is commonly obtained in small/medium scale cheese plants and, although it has little to no commercial value, it is produced in relatively high volumes, which results in a complex disposal process. Therefore, the objective of this work was to characterize the nutritional composition of two concentrated deproteinized wheys (sheep and goat), obtained through ultrafiltration/diafiltration with a 10 kDa membrane followed by freezedrying. From the nutritional standpoint, it was possible to observe that after ultrafiltration, samples were mostly comprised of protein 75-58% (w/w). Samples also exhibited a low mineral and lactose contents (1.7-3.9% (w/w) and 0.93 -69%(w/w), respectively). The fatty acid composition profile was predominantly composed of short- and medium chain fatty acids including butyric, caproic and caprylic acids, with long chain fatty acids, such as palmitoleic, rumenic, trans vaccenic, docosapentaenoic and docosahexaenoic acids being also detected in both goat and sheep Sorelho, but in the goat Sorelho the concentration was bigger of these fatty acids. When regarding the amino acids identified aspartic acid, glutamic acid, alanine and tyrosine were the most predominant amino acids found in the sheep Sorelho while in the goat Sorelho aspartic acid, glutamic acid, asparagine, threonine and valine were prevalent. Overall, these results provide some insights into Sorelhos’ potential usage as source of bioactive compounds for posterior incorporation into foods.