Browsing by Author "Coscueta, Ezequiel"
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- Bioactive peptides produced from the mussel mytilus galloprovincialis by enzymatic hydrolysis with corolasePublication . Cunha, Sara; Castro, Rita de; Coscueta, Ezequiel; Pintado, Manuela
- A circular economy approach to leather by products valorization towards a zero-waste processPublication . Bonifácio-Lopes, Teresa; Afonso, Tiago Barros; Coscueta, Ezequiel; Costa, Eduardo M.; Pintado, ManuelaIntroduction: The tanning industry has a significant waste production and is a major contributor to environmental pollution; The adoption of circular economy principles will help repurposing by-products with a focus on reutilizing leather remnants; With this approach, the objective is to minimize environmental damage and effectively manage waste; This will contribute to sustainability with a reduction of overall waste and will align with UN sustainability goal 12 (Ensure sustainable consumption and production patterns) through the re-use of by products. Objectives: Exploration of different enzymes and conditions to enhance leather industry hydrolysis process to achieve a more sustainable and circular approach; Creation of versatile functional coatings within the leather industry; Utilization of commercial enzyme (Protabate P) and alternative enzymes (Bromelain and Alcalase) to validate its use; Valorization of protein-rich leather by-products. Conclusion: Protabate P 10% 24 h had higher hydrolysis degree (49.66%) and higher total protein (55103.43 μg protein/mL) than all the other enzymes. Regarding antioxidant activity, Alcalase 10% 24 h showed higher values (39.32 μM Trolox equivalents/mL). Apart from total protein, Bromelain 10% 24 h showed the lowest values for hydrolysis degree and antioxidant activity. Between time 0 and pre-final, molecules with MW <0.2 kDa consistently have a higher area. Conducting an enzyme inactivation (2 h/90 ºC) might result in molecule aggregation, leading to an increase in molecules with MW exceeding 50 kDa. Re-using leather byproducts can help the tanning industry to reduce the waste and achieve the UN sustainability goal nr 12. This study outlines the potential of re-using leather by-products to create versatile functional coatings through a circular and more sustainable process.
- A circular economy approach to leather by products valorization towards a zero-waste processPublication . Bonifácio-Lopes, Teresa; Afonso, Tiago Barros; Coscueta, Ezequiel; Costa, Eduardo; Pintado, ManuelaThe tanning industry, known for its significant waste production, has a great environmental footprint and is one of the main contributors of environmental pollution. To address this concern, this industry has been actively adopting circular economy principles to repurpose the by-products it generates. One of its key focuses is the reutilization of leather by-products, aiming to minimize environmental harm through effective waste management and resource conservation. This strategy shift not only contributes to reducing overall waste but also aligns itself with the 12nd UN sustainability goal (Ensure sustainable consumption and production patterns) by the re-use of its own byproducts. This study focused on exploring different enzymes and hydrolysis conditions for the repurposing of leather byproducts. The objective is to enhance the existing leather industry hydrolysis process, with a primary focus on achieving a more sustainable and circular approach towards the creation of versatile functional coatings within the leather industry. For this purpose, hydrolysis processes using commercial (ProtaBate P) and alternative enzymes (Bromelain and Alcalase) were optimized targeting its reduction from 24 to 6h reactions. Samples MW was determined through SE-HPLC analyses and hydrolysis percentage (TNBS), total protein (BCA) and antioxidant activity (ORAC) were also evaluated. The results demonstrate that all the enzymes had an effect in the leather by-product. It is also possible to conclude that Alcalase provided a better hydrolysis performance than ProtaBate P and Bromelain. Alcalase had higher hydrolysis percentage (70.12%), MWs between 10 and 50 kDa and a higher antioxidant activity (34.51 vs 32.87 µM Trolox equivalent/mL sample). This study outlines the potential of re-using leather by-products to create versatile functional coatings through a circular and more sustainable process.
- Cod skin collagen extraction and characterizationPublication . Coscueta, Ezequiel; Brassesco, María; Pintado, Manuela
- Cod skin collagen extraction and characterizationPublication . Coscueta, Ezequiel; Brassesco, María; Pintado, Manuela
- Defatted nannochloropsis oculata biomass: waste or resource?Publication . Sousa, Sérgio; Machado, Manuela; Coscueta, Ezequiel; Ferreira, Andreia S.; Nunes, Cláudia; Coimbra, Manuel A.; Freitas, Ana C.; Carvalho, Ana P.; Gomes, Ana M.Microalgae are recognized as a valuable source of a panoply of compounds. In addition to the extensively investigated lipid fraction comprising polyunsaturated fatty acids (PUFA), microalgae biomass also encompasses other compounds with potentially relevant biological activities. This work innovatively repurposes the defatted biomass (DB) of Nannochloropsis oculata, highlighting its potential value beyond the lipid fraction. By subjecting the DB to enzymatic hydrolysis, we explored an underutilized resource, potentially reducing waste and promoting sustainable bioprocessing. The resulting soluble fraction was chemically characterized and a comprehensive assessment of its chemical and biological activities was performed to ascertain its potential applications. Those included antioxidant, anti-hypertensive, and antidiabetic capacities, as well as potential metabolic inhibition, anti-inflammatory, and antimicrobial activities. The chemical characterization revealed the presence of several low molecular weight peptides (<1.2 kDa), as well as polysaccharides. The DB exhibited a relevant antioxidant capacity of 3.15 μmolTrolox equivalent(TE)/mgfreeze-dried biomass(FDB) and an IC50 of 77.3 ± 0.3 μgprotein/mL concerning angiotensin-I converting enzyme inhibitory activity, while α-glucosidase activity was inhibited by 19.4 %. Biological activities revealed no relevant inhibition of metabolic activity, an immunosuppression potential and anti-inflammatory activity (decreased expression of all pro-inflammatory cytokines in lipopolysaccharide (LPS)-induced inflammation) was also observed. Moreover, significant antimicrobial activity was observed, particularly against Gram-positive bacteria. These results underscored the potential of the DB to be utilized within a biorefinery concept, thereby transforming it into a resource (co-product) rather than considering it as waste. This study is groundbreaking due to its integrative approach, being the first to report the potential bioactivities of non-lipid extracts from N. oculata cultivated under modulated stress conditions.
- Enzymatic hydrolysis of soymilk byproduct (Okara) by proteases extracted from Cynara CardunculusPublication . Voss, Glenise B.; Coscueta, Ezequiel; Valente, Luisa M. P.; Pintado, Maria M.Soybeans are the main oilseed produced and consumed worldwide. Currently, Brazil, Argentina and China are the largest producers of soybeans. This oilseed has great commercial interest, especially, because of the oil extraction, soy proteins and soymilk. Soymilk is used as a base in a wide variety of products, including tofu, soy yogurt and cheese. This soybean product is achieved by aqueous extraction of whole soybeans. During this process, a by-product known as okara rich in fiber, protein and fat, is obtained (3,4 5). Okara is produced in high amounts, since for each 1 kg of processed soybeans about 1.1 kg of okara is produced (1). The okara by-product has a high nutritive value, as previously mentioned. However, during soymilk manufacturing, this by-product is submitted to a severe heat treatment, which causes a large protein denaturation and the resulting okara protein isolate has poor solubility, which restricts its direct use in food. Studies reported that protein can be produced from the okara and that the protein isolates are characterized as having good amino acid profile and showing good digestibility (2). Beyond other functional properties emulsification, foaming and binding properties were comparable to those of commercial soy isolate. Therefore, the main objective of this work was to study the enzymatic hydrolysis of two okara substrates (dry okara, previously autoclaved (OA) and not autoclaved (ONA)), achieved by an enzymatic aqueous extract of Cynara cardunculus. The okara used in this study was provided by a local soybean producer in Portugal (NUTRE). After collection the okara samples were divided in two batches and one was submitted to heat treatment (1 atm, 121 ° C for 20 minutes) and the other was not heat treated. Finally, both were dried at 65 °C until constant weight and then milled with a 1 mm mesh. Two hydrolysis factors (reaction time and ratio of enzyme/substrate, E/S) were selected. The following parameters were analyzed: degree of hydrolysis (DH), antioxidant activity (ABTS) and the profile of hydrolysis (RH) determined by FPLC (fast protein liquid chromatography gel filtration). The hydrolysis was performed using commercial crude extract of Cynara cardunculus at 55 ° C and pH 5.2 for 2.5 to 5 h. No significant differences on the DH were observed between OA and ONA or between hydrolysis factors tested using the method of o-phthaldialdehyde (OPA). However, chromatographic analysis by FPLC showed strong protein hydrolysis after 2.5 h with formation of peptides fractions, with no significant difference between OA and ONA, but showing increasing concentration according increasing E/S rations. The antioxidant activity results showed a higher antioxidant potential for OA than for ONA and directly proportional to the time of hydrolysis, which proves that previous protein denaturation may promote the release of more antioxidant peptides. Therefore, it can be concluded that the hydrolysis of okara protein with C. cardunculus generated peptide extracts with relevant antioxidant activity, which is affected by the denaturation state of the proteins.
- Harnessing agrifood by-products for sustainable protein development: a focus on circular economy and agriculturePublication . Paupério, Ana Isabel; Brassesco, María Emilia; Coscueta, Ezequiel; Ferreira, João Paulo; Pintado, Manuela
- Hydrolysates of mytilus galloprovincialis as a source of bioactive compoundsPublication . Cunha, Sara; Coscueta, Ezequiel; Nova, Paulo; Costa-Pinto, Ana Rita; Sarmento, Bruno; Pintado, Manuela
- Influence of temperature and pH on antioxidant and antihypertensive activities of a fish protein hydrolysatePublication . Moreira, Maria João Pinho; Ribeiro, Tânia Bragança; Coscueta, Ezequiel; Pintado, Manuela
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