Browsing by Author "Andrade, J. C."
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- 1,3-Propanediol continuous production by Clostridium butyricum VP1 1718: effect of dilution rate and substrate concentrationPublication . Gonzalez-Pajuelo, M.; Ribeiro-Cruz, A. P.; Sousa- Monagas, C. M.; Andrade, J. C.; Vasconcelos, I.
- Akkermansia muciniphila antimicrobial susceptibility profilePublication . Barbosa, J. C.; Machado, D.; Almeida, D.; Andrade, J. C.; Freitas, A. C.; Gomes, A. M.
- Analyzing the potential of selected gut commensal strains to produce antimicrobial peptides: phenotypic and in silico approachesPublication . Fonseca, M.; Machado, D.; Vedor, R.; Andrade, J. C.; Gomes, A. M.; Barbosa, J. C.The World Health Organization estimates that the number of antibiotic resistance-related deaths could reach 10 million by 2050 [1]. Given the dynamics and high diversity of microorganisms that inhabit the gastrointestinal tract, this is the ideal place to discover new antimicrobial peptides (AMP) to replace traditional antibiotics [2]. Among the most extensively studied members are the commensal strains Akkermansia muciniphila DSM 22959 and Faecalibacterium duncaniae DSM 17677, which are reported to have a beneficial impact on intestinal health [3;4]. Antimicrobial peptides are small molecules that act as the first line of defense against microbial invaders, playing a vital role in the innate immune system [5]. One approach to identify new strategies to combat antimicrobial resistance is to evaluate the ability of these bacteria to produce AMP.
- Antioxidative peptides: trends and perspectives for future researchPublication . Freitas, A. C.; Andrade, J. C.; Silva, F. M.; Rocha-Santos, T. A. P.; Duarte, A. C.; Gomes, Ana M. P.In recent years, much attention has been given to dietary antioxidants, especially polyphenols. Several peptides derived from protein molecules have also been found to show antioxidant capacity along with other biological properties and thus there is an increasing interest in these compounds as health promoters. This review summarizes and discusses the main sources of antioxidative peptides with focus on food-derived peptides (animal, plant and marine sources), methods of preparation, antioxidant capacity evaluation as well as their proposed mechanisms of action. A discussion of the potential health effects and comments on the different applications for these antioxidants and their potential research interest are also subject of this review.
- Characterization of potential CLA-producing strains according to LA tolerancePublication . Fontes, A. L.; Pimentel, L. L.; Salsinha, A. S.; Cardoso, B.; Andrade, J. C.; Rodríguez-Alcalá, L. M.; Gomes, A. M.Introduction: Linoleic acid (LA) present in ruminants diet goes through a series of reactions within the rumen, called the biohydrogenation pathway. This pathway ends up converting LA to stearic acid (C18), but several intermediate products are formed, such as conjugated linoleic acid (CLA) isomers, which have been extensively studied for their potential bioactive properties1,2. Different ruminal bacteria are involved in this process, however, strains of lactobacilli, bifidobacteria and propionibacteria isolated from human intestine and dairy products have also demonstrated the ability to produce those fatty acids3. Most in vitro CLA production assays normally test potential producing strains by culturing them in the presence of a pure LA solution at 0.5 mg/mL or less4. However, possibly there are some strains that can tolerate higher concentrations of LA and if they are producers, CLA yields may probably be higher. Objective: The major aim of this work was to determine the maximum LA concentration that potential CLA-producing strains can tolerate in further in vitro production assays. Conclusions: In conclusion, potential CLA-producing strains exhibit different LA tolerance degrees, being lactobacilli the more tolerant at the highest LA concentration assayed. Currently overlooked, this is an important parameter to consider in future production tests.
- Continuous fermentation of glycerol: a comparative study of two strains of clostridium acetobutylicumPublication . Andrade, J. C.; Vasconcelos, I.
- Continuous fermentation of glycerol: a comparative study of two strains of clostridium acetobutylicumPublication . Andrade, J. C.; Vasconcelos, I.
- Hydrophobicity and aggregation properties of gut commensals faecalibacterium duncaniae DSM 17677 and akkermansia muciniphila DSM 22959Publication . Fonseca, M.; Machado, D.; Vedor, R.; Barbosa, J. C.; Andrade, J. C.; Gomes, A. M.Probiotics have been emerging as a promising approach to prevent and control foodborne diseases [1]. In the last years, the bacterial species isolated from gut microbiota, such as Faecalibacterium spp. and Akkermansia muciniphila, have been proposed as novel probiotic candidates [2]. The cell surface hydrophobicity, auto-aggregation and co-aggregation with pathogens are considered desirable characteristics of probiotic strains and these properties may be used in preliminary screening to identify potential probiotic microorganisms appropriate for human or animal use [3].
- Incorporation of Akkermansia muciniphila DSM 22959 in chocolate matricesPublication . Vedor, R.; Machado, D.; Barbosa, J. C.; Andrade, J. C.; Gomes, A. M.
- Investigating the susceptibility of the next generation probiotic faecalibacterium prausnitzii under stress conditionsPublication . Machado, D.; Domingos, M.; Almeida, D.; Barbosa, J. C.; Andrade, J. C.; Freitas, A. C.; Gomes, A. M.