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Advisor(s)
Abstract(s)
A flow injection analysis (FIA) system was
developed for the determination of phosphate efflux from wine lactic acid bacteria (Oenococus oeni and Lactobacillus hilgardii) as an indication of cell membrane
damage. The system allowed the direct injection of the cell suspension, avoiding the filtration step, with minimumsample treatment andminimized reagent consumption.
The developed system is characterized by
a linear concentration zone between 3.2310 5 and
4.84104 mol L1 PO4
3 and repeatability better than
2.9%. Bacterial suspensions were exposed to a chemical stress with phenolic acids and injected in the FIAsystem
at regular intervals. The extracellular concentration of phosphate was measured spectrophotometrically. The
experimental results obtained indicate that hydroxycinnamic acids (p-coumaric, ferulic and caffeic) induced faster phosphate leakage rates than hydroxybenzoic
acids (vanillic and p-hydroxybenzoic) in both strains tested, which could be related to their higher lipophilic character.
Description
Keywords
Flow injection analysis Membrane damage Phenolic acids Phosphate efflux Wine lactic acid bacteria
Citation
"Microchimica acta". ISSN 0026-3672. 159: 1-2 (2007) 87-93
Publisher
Springer-Verlag