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Development of a flow injection method for monitoring cell membrane damage of wine lactic acid bacteria

2007-04Journal article Restricted access
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dc.contributor.authorTorres, André F.
dc.contributor.authorMesquita, Paulo A. R.
dc.contributor.authorCampos, Francisco M.
dc.contributor.authorCouto, José A.
dc.contributor.authorTóth, Ildikó V.
dc.contributor.authorRangel, António O. S. S.
dc.contributor.authorHogg, Tim
dc.date.accessioned2011-09-12T16:45:04Z
dc.date.available2011-09-12T16:45:04Z
dc.date.issued2007-04
dc.description.abstractA flow injection analysis (FIA) system was developed for the determination of phosphate efflux from wine lactic acid bacteria (Oenococus oeni and Lactobacillus hilgardii) as an indication of cell membrane damage. The system allowed the direct injection of the cell suspension, avoiding the filtration step, with minimumsample treatment andminimized reagent consumption. The developed system is characterized by a linear concentration zone between 3.2310 5 and 4.84104 mol L1 PO4 3 and repeatability better than 2.9%. Bacterial suspensions were exposed to a chemical stress with phenolic acids and injected in the FIAsystem at regular intervals. The extracellular concentration of phosphate was measured spectrophotometrically. The experimental results obtained indicate that hydroxycinnamic acids (p-coumaric, ferulic and caffeic) induced faster phosphate leakage rates than hydroxybenzoic acids (vanillic and p-hydroxybenzoic) in both strains tested, which could be related to their higher lipophilic character.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citation"Microchimica acta". ISSN 0026-3672. 159: 1-2 (2007) 87-93por
dc.identifier.doi10.1007/s00604-007-0763-6
dc.identifier.urihttp://hdl.handle.net/10400.14/5386
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringer-Verlagpor
dc.subjectFlow injection analysispor
dc.subjectMembrane damagepor
dc.subjectPhenolic acidspor
dc.subjectPhosphate effluxpor
dc.subjectWine lactic acid bacteriapor
dc.titleDevelopment of a flow injection method for monitoring cell membrane damage of wine lactic acid bacteriapor
dc.typejournal article
dspace.entity.typePublication
person.familyNameMorais Sarmento de Campos
person.familyNameCouto
person.familyNameTóth
person.familyNameSantos Silva Rangel
person.familyNameHogg
person.givenNameFrancisco Manuel
person.givenNameJosé António
person.givenNameIldikó
person.givenNameAntónio Osmaro
person.givenNameTim
person.identifier1393111
person.identifier.ciencia-id4815-6AA3-E339
person.identifier.ciencia-id0E19-CD42-2983
person.identifier.ciencia-idD61D-7CFB-178A
person.identifier.ciencia-idFF11-88CC-8BD4
person.identifier.orcid0000-0001-8332-5043
person.identifier.orcid0000-0002-3156-1412
person.identifier.orcid0000-0002-7342-1063
person.identifier.orcid0000-0002-6486-8947
person.identifier.orcid0000-0003-2017-4131
person.identifier.ridAAZ-2861-2021
person.identifier.ridG-1733-2010
person.identifier.ridJXY-0365-2024
person.identifier.scopus-author-id35614525200
person.identifier.scopus-author-id35512332800
person.identifier.scopus-author-id7004827387
person.identifier.scopus-author-id7005603860
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication6b001d48-8bde-41c3-afa0-d6473fe74edf
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relation.isAuthorOfPublication12b8092d-fe59-408b-8058-47369a97d229
relation.isAuthorOfPublication.latestForDiscovery12b8092d-fe59-408b-8058-47369a97d229

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