Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/3388
Título: Metabolic engineering of clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol
Autor: González-Pajuelo, María
Meynial-Salles, Isabelle
Mendes, Filipa
Andrade, Jose Carlos
Vasconcelos, Isabel
Soucaille, Philippe
Palavras-chave: 3-propanediol
Clostridium acetobutylicum
Metabolic engineering
Vitamin B12-free biological process
Data: 2005
Editora: Elsevier
Citação: GONZÁLEZ-PAJUELO, María ...[et al.] - Metabolic engineering of clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol. Metabolic Engineering. ISSN 1096-7176. Vol. 7, n.º 5-6 (2005), p. 329–336
Resumo: Clostridium butyricum is to our knowledge the best natural 1,3-propanediol producer from glycerol and the only microorganism identified so far to use a coenzyme B12-independent glycerol dehydratase. However, to develop an economical process of 1,3-propanediol production, it would be necessary to improve the strain by a metabolic engineering approach. Unfortunately, no genetic tools are currently available for C. butyricum and all our efforts to develop them have been so far unsuccessful. To obtain a better ‘‘vitamin B12-free’’ biological process, we developed a metabolic engineering strategy with Clostridium acetobutylicum. The 1,3- propanediol pathway from C. butyricum was introduced on a plasmid in several mutants of C. acetobutylicum altered in product formation. The DG1(pSPD5) recombinant strain was the most efficient strain and was further characterized from a physiological and biotechnological point of view. Chemostat cultures of this strain grown on glucose alone produced only acids (acetate, butyrate and lactate) and a high level of hydrogen. In contrast, when glycerol was metabolized in chemostat culture, 1,3-propanediol became the major product, the specific rate of acid formation decreased and a very low level of hydrogen was observed. In a fed-batch culture, the DG1(pSPD5) strain was able to produce 1,3-propanediol at a higher concentration (1104mM) and productivity than the natural producer C. butyricum VPI 3266. Furthermore, this strain was also successfully used for very long term continuous production of 1,3-propanediol at high volumetric productivity (3 g L⁻¹ h⁻¹) and titer (788 mM).
URI: http://hdl.handle.net/10400.14/3388
Versão do Editor: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WN3-4GV988D-2&_user=2460358&_coverDate=11%2F30%2F2005&_rdoc=2&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info%28%23toc%236951%232005%23999929994%23613676%23FLA%23display%23Volume%29&_cdi=6951&_sort=d&_docanchor=&_ct=14&_acct=C000057417&_version=1&_urlVersion=0&_userid=2460358&md5=6e9705961b1b6fb012ca4f57229d4252&searchtype=a
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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