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Metabolic engineering of clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol

2005Journal article Open access
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dc.contributor.authorGonzález-Pajuelo, María
dc.contributor.authorMeynial-Salles, Isabelle
dc.contributor.authorMendes, Filipa
dc.contributor.authorAndrade, José Carlos
dc.contributor.authorVasconcelos, Isabel
dc.contributor.authorSoucaille, Philippe
dc.date.accessioned2010-11-11T16:46:47Z
dc.date.available2010-11-11T16:46:47Z
dc.date.issued2005
dc.description.abstractClostridium butyricum is to our knowledge the best natural 1,3-propanediol producer from glycerol and the only microorganism identified so far to use a coenzyme B12-independent glycerol dehydratase. However, to develop an economical process of 1,3-propanediol production, it would be necessary to improve the strain by a metabolic engineering approach. Unfortunately, no genetic tools are currently available for C. butyricum and all our efforts to develop them have been so far unsuccessful. To obtain a better ‘‘vitamin B12-free’’ biological process, we developed a metabolic engineering strategy with Clostridium acetobutylicum. The 1,3- propanediol pathway from C. butyricum was introduced on a plasmid in several mutants of C. acetobutylicum altered in product formation. The DG1(pSPD5) recombinant strain was the most efficient strain and was further characterized from a physiological and biotechnological point of view. Chemostat cultures of this strain grown on glucose alone produced only acids (acetate, butyrate and lactate) and a high level of hydrogen. In contrast, when glycerol was metabolized in chemostat culture, 1,3-propanediol became the major product, the specific rate of acid formation decreased and a very low level of hydrogen was observed. In a fed-batch culture, the DG1(pSPD5) strain was able to produce 1,3-propanediol at a higher concentration (1104mM) and productivity than the natural producer C. butyricum VPI 3266. Furthermore, this strain was also successfully used for very long term continuous production of 1,3-propanediol at high volumetric productivity (3 g L⁻¹ h⁻¹) and titer (788 mM).por
dc.description.versionN/A
dc.identifier.citationGONZÁLEZ-PAJUELO, María ...[et al.] - Metabolic engineering of clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol. Metabolic Engineering. ISSN 1096-7176. Vol. 7, n.º 5-6 (2005), p. 329–336por
dc.identifier.doi10.1016/j.ymben.2005.06.001
dc.identifier.urihttp://hdl.handle.net/10400.14/3388
dc.language.isoengpor
dc.publisherElsevierpor
dc.subject3-propanediolpor
dc.subjectGlycerolpor
dc.subjectClostridium acetobutylicumpor
dc.subjectMetabolic engineeringpor
dc.subjectVitamin B12-free biological processpor
dc.titleMetabolic engineering of clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerolpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleMetabolic Engineering
person.familyNameAndrade
person.familyNameVasconcelos
person.givenNameJosé
person.givenNameIsabel
person.identifier684026
person.identifier.ciencia-id2D1D-00CA-016F
person.identifier.ciencia-id851B-41B9-4746
person.identifier.orcid0000-0002-2403-6062
person.identifier.orcid0000-0002-0565-3516
person.identifier.ridN-3870-2013
person.identifier.scopus-author-id55415967800
person.identifier.scopus-author-id7003767794
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb5156d42-c49f-4991-8aa9-fc77cfff8a33
relation.isAuthorOfPublication251ce926-a408-483f-a3df-acebe6631477
relation.isAuthorOfPublication.latestForDiscoveryb5156d42-c49f-4991-8aa9-fc77cfff8a33

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