Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/3002
Título: Linkage of cytosolic peroxiredoxin 2 to erythrocyte membrane imposed by hydrogen peroxide-induced oxidative stress
Autor: Rocha, Susana
Costa, Elísio
Coimbra, Susana
Nascimento, Henrique
Catarino, Cristina
Rocha-Pereira, Petronila
Quintanilha, Alexandre
Belo, Luís
Santos-Silva, Alice
Palavras-chave: Peroxiredoxin 2
Erythrocyte membrane
Oxidative stress
Hemoglobin autoxidation
Data: 2009
Editora: Elsevier
Citação: ROCHA, Susana ...[et al.] - Linkage of cytosolic peroxiredoxin 2 to erythrocyte membrane imposed by hydrogen peroxide-induced oxidative stress. Blood Cells, Molecules, and Diseases. ISSN 1079-9796. Vol. 43, n.º 1 (2009), p. 68–73
Resumo: Human erythrocyte peroxiredoxin 2 (Prx2) is a typical 2-cys cytosolic peroxiredoxin with thiol-dependent hydrogen peroxide scavenger activity. In a previous work, we reported Prx2 erythrocyte membrane linkage in some Hereditary Spherocytosis patients and that it seemed to be related to oxidative stress. The aim of the present work was to determine if Prx2 linkage to erythrocyte membrane could be induced by oxidative stress mediated by H2O2 and to further understand how and why this process occurs. We performed in vitro assays in which catalase or both Hb autoxidation and catalase were inhibited, under H2O2-induced oxidative stress conditions. Erythrocyte membrane linked Prx2 was detected by immunoblotting and quantified by densitometry. As oxidative stress markers, we determined membrane bound hemoglobin and lipid peroxidation, and we found that their values increased with H2O2 concentration. Prx2 linkage to the membrane also rose with increasing H2O2 concentration, and was only observed when the oxidized form of the enzyme was present in the cytosol. Oxidized Hb and Prx2 membrane linkages appear to be independent processes, although, both result from oxidative stress and may be useful as oxidative stress and/or erythrocyte damage/senescence markers.
URI: http://hdl.handle.net/10400.14/3002
Versão do Editor: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBV-4W329H8-2&_user=2460358&_coverDate=08%2F31%2F2009&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000057417&_version=1&_urlVersion=0&_userid=2460358&md5=0599861281940a9fd00ad669ab98da72&searchtype=a
Aparece nas colecções:ICS(P) - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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