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Linkage of cytosolic peroxiredoxin 2 to erythrocyte membrane imposed by hydrogen peroxide-induced oxidative stress

2009Journal article Restricted access
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dc.contributor.authorRocha, Susana
dc.contributor.authorCosta, Elísio
dc.contributor.authorCoimbra, Susana
dc.contributor.authorNascimento, Henrique
dc.contributor.authorCatarino, Cristina
dc.contributor.authorRocha-Pereira, Petronila
dc.contributor.authorQuintanilha, Alexandre
dc.contributor.authorBelo, Luís
dc.contributor.authorSantos-Silva, Alice
dc.date.accessioned2010-10-16T11:51:14Z
dc.date.available2010-10-16T11:51:14Z
dc.date.issued2009
dc.description.abstractHuman erythrocyte peroxiredoxin 2 (Prx2) is a typical 2-cys cytosolic peroxiredoxin with thiol-dependent hydrogen peroxide scavenger activity. In a previous work, we reported Prx2 erythrocyte membrane linkage in some Hereditary Spherocytosis patients and that it seemed to be related to oxidative stress. The aim of the present work was to determine if Prx2 linkage to erythrocyte membrane could be induced by oxidative stress mediated by H2O2 and to further understand how and why this process occurs. We performed in vitro assays in which catalase or both Hb autoxidation and catalase were inhibited, under H2O2-induced oxidative stress conditions. Erythrocyte membrane linked Prx2 was detected by immunoblotting and quantified by densitometry. As oxidative stress markers, we determined membrane bound hemoglobin and lipid peroxidation, and we found that their values increased with H2O2 concentration. Prx2 linkage to the membrane also rose with increasing H2O2 concentration, and was only observed when the oxidized form of the enzyme was present in the cytosol. Oxidized Hb and Prx2 membrane linkages appear to be independent processes, although, both result from oxidative stress and may be useful as oxidative stress and/or erythrocyte damage/senescence markers.por
dc.identifier.citationROCHA, Susana ...[et al.] - Linkage of cytosolic peroxiredoxin 2 to erythrocyte membrane imposed by hydrogen peroxide-induced oxidative stress. Blood Cells, Molecules, and Diseases. ISSN 1079-9796. Vol. 43, n.º 1 (2009), p. 68–73por
dc.identifier.doi10.1016/j.bcmd.2009.03.002
dc.identifier.eid68649102973
dc.identifier.pmid19375361
dc.identifier.urihttp://hdl.handle.net/10400.14/3002
dc.identifier.wos000271076500013
dc.language.isoengpor
dc.publisherElsevierpor
dc.subjectPeroxiredoxin 2por
dc.subjectErythrocyte membranepor
dc.subjectOxidative stresspor
dc.subjectHemoglobin autoxidationpor
dc.titleLinkage of cytosolic peroxiredoxin 2 to erythrocyte membrane imposed by hydrogen peroxide-induced oxidative stresspor
dc.typejournal article
dspace.entity.typePublication
person.familyNameBelo
person.givenNameLuís
person.identifier.ciencia-idC319-EED1-E15D
person.identifier.orcid0000-0002-3941-6850
person.identifier.ridK-5878-2013
person.identifier.scopus-author-id6602879850
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3654b7b5-7ad2-4f2f-9114-93dc094f8e9e
relation.isAuthorOfPublication.latestForDiscovery3654b7b5-7ad2-4f2f-9114-93dc094f8e9e

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