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Advisor(s)
Abstract(s)
Two up-flow fixed bed reactors (UFBR) were
operated for 8 months treating a model synthetic wastewater containing 2-fluorobenzoate (2-FB) and dichloromethane
(DCM). The stability of the reactors under dynamic conditions, that is, sequentially alternating pollutants (SAP), shock loads, and starvation periods was assessed. Two
support materials were used: expanded clay (EC) that does not adsorb 2-FB or DCM, and granular-activated carbon (GAC) that adsorbs 180 mg gg⁻¹ of 2-FB and 390 mg gg⁻¹ of DCM. The reactors were inoculated with a 2-FB-degrading strain (FB2) and a DCM degrader (TM1). 2-FB was fed at organic loads ranging from 0 to 800 mg L⁻¹ d⁻¹, while DCM was fed at 0–250 mg L⁻¹ d⁻¹. 2-FB or DCM were never detected at the outlet of the GAC reactor, while in the EC reactor outlet small amounts were observed. Nevertheless, the highest biological elimination capacity was observed in
the EC reactor (over 700 mg L⁻¹ d⁻¹ of 2-FB). DGGE analysis revealed a fairly stable bacterial community with the largest shifts occurring during starvation periods and changes in feed composition. Several bacterial strains isolated from the reactors showed capacity for 2-FB degradation, while only strain TM1 degraded DCM.
Description
Keywords
Up-flow fixed bed reactor 2-fluorobenzoate Dichloromethane Biodegradation sequentially alternating pollutants Microbial dynamics
Pedagogical Context
Citation
"Biotechnology and Bioengineering". ISSN 0006-3592. 99: 4 (2008) 800-810
Publisher
Wiley Blackwell