Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/2779
Título: Effects of chitooligosaccharides on human red blood cell morphology and membrane protein structure
Autor: Fernandes, João C.
Eaton, Peter
Nascimento, Henrique
Belo, Luís
Rocha, Susana
Vitorino, Rui
Amado, Francisco
Gomes, Joana
Santos-Silva, Alice
Pintado, Manuela E.
Malcata, F. Xavier
Data: 2008
Editora: American Chemical Society
Citação: "Biomacromolecules". ISSN 1525-7797. 9: 12 (2008) 3346-3352
Resumo: Recent studies of chitosan have increased the interest in its conversion to chitooligosaccharides (COSs) because these compounds are water-soluble and have potential use in several biomedical applications. Furthermore, such oligomers may be more advantageous than chitosans because of their much higher absorption profiles at the intestinal level, which permit their facilitated access to systemic circulation and potential distribution throughout the entire human body. In that perspective, it is important to clarify their effect on blood further, namely, on human red blood cells (RBCs). The aim of this work was thus to study the effect of two COS mixtures with different molecular weight (MW) ranges, <3 and <5 kDa, at various concentrations (5.0-0.005 mg/mL) on human RBCs. The interactions of these two mixtures with RBC membrane proteins and with hemoglobin were assessed, and the RBC morphology and surface structure were analyzed by optical microscopy (OM) and atomic force microscopy (AFM). In the presence of either COS mixture, no significant hemolysis was observed; however, at COS concentrations >0.1 mg/mL, changes in membrane binding hemoglobin were observed. Membrane protein changes were also observed with increasing COS concentration, including a reduction in both α- and β-spectrin and in band 3 protein, and the development of three new protein bands: peroxiredoxin 2, calmodulin, and hemoglobin chains. Morphologic evaluation by OM showed that at high concentrations COSs interact with RBCs, leading to RBC adhesion, aggregation, or both. An increase in the roughness of the RBC surface with increasing COS concentration was observed by AFM. Overall, these findings suggest that COS damage to RBCs was dependent on the COS MW and concentration, and significant damage resulted from either a higher MW or a greater concentration (>0.1 mg/mL).
URI: http://hdl.handle.net/10400.14/2779
Versão do Editor: http://pubs.acs.org/doi/abs/10.1021/bm800622f
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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