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Effects of chitooligosaccharides on human red blood cell morphology and membrane protein structure

2008Journal article Restricted access
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dc.contributor.authorFernandes, João C.
dc.contributor.authorEaton, Peter
dc.contributor.authorNascimento, Henrique
dc.contributor.authorBelo, Luís
dc.contributor.authorRocha, Susana
dc.contributor.authorVitorino, Rui
dc.contributor.authorAmado, Francisco
dc.contributor.authorGomes, Joana
dc.contributor.authorSantos-Silva, Alice
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2010-10-11T12:08:14Z
dc.date.available2010-10-11T12:08:14Z
dc.date.issued2008
dc.description.abstractRecent studies of chitosan have increased the interest in its conversion to chitooligosaccharides (COSs) because these compounds are water-soluble and have potential use in several biomedical applications. Furthermore, such oligomers may be more advantageous than chitosans because of their much higher absorption profiles at the intestinal level, which permit their facilitated access to systemic circulation and potential distribution throughout the entire human body. In that perspective, it is important to clarify their effect on blood further, namely, on human red blood cells (RBCs). The aim of this work was thus to study the effect of two COS mixtures with different molecular weight (MW) ranges, <3 and <5 kDa, at various concentrations (5.0-0.005 mg/mL) on human RBCs. The interactions of these two mixtures with RBC membrane proteins and with hemoglobin were assessed, and the RBC morphology and surface structure were analyzed by optical microscopy (OM) and atomic force microscopy (AFM). In the presence of either COS mixture, no significant hemolysis was observed; however, at COS concentrations >0.1 mg/mL, changes in membrane binding hemoglobin were observed. Membrane protein changes were also observed with increasing COS concentration, including a reduction in both α- and β-spectrin and in band 3 protein, and the development of three new protein bands: peroxiredoxin 2, calmodulin, and hemoglobin chains. Morphologic evaluation by OM showed that at high concentrations COSs interact with RBCs, leading to RBC adhesion, aggregation, or both. An increase in the roughness of the RBC surface with increasing COS concentration was observed by AFM. Overall, these findings suggest that COS damage to RBCs was dependent on the COS MW and concentration, and significant damage resulted from either a higher MW or a greater concentration (>0.1 mg/mL).por
dc.identifier.citation"Biomacromolecules". ISSN 1525-7797. 9: 12 (2008) 3346-3352por
dc.identifier.doi10.1021/bm800622f
dc.identifier.urihttp://hdl.handle.net/10400.14/2779
dc.language.isoengpor
dc.publisherAmerican Chemical Societypor
dc.titleEffects of chitooligosaccharides on human red blood cell morphology and membrane protein structurepor
dc.typejournal article
dspace.entity.typePublication
person.familyNameFernandes
person.familyNameBelo
person.familyNamePintado
person.familyNameMalcata
person.givenNameJoão
person.givenNameLuís
person.givenNameMaria Manuela
person.givenNameFrancisco
person.identifier456608
person.identifier.ciencia-id881D-7FFD-DD7D
person.identifier.ciencia-idC319-EED1-E15D
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-1556-1698
person.identifier.orcid0000-0002-3941-6850
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridK-3126-2013
person.identifier.ridK-5878-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id35274726800
person.identifier.scopus-author-id6602879850
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id7102542478
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication3654b7b5-7ad2-4f2f-9114-93dc094f8e9e
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