Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/2695
Título: Study of the antibacterial effects of chitosans on bacillus cereus (and its spores) by atomic force microscopy imaging and nanoindentation
Autor: Fernandes, João C.
Eaton, Peter
Gomes, Ana M.
Pintado, Manuela E.
Malcata, F. Xavier
Palavras-chave: Atomicforcemicroscopy(AFM)
Data: 2009
Editora: Elsevier
Citação: "Ultramicroscopy". ISSN 0304-3991. 109: 8 (2009) 854–860
Resumo: Bacillus cereus is a Gram-positive, spore-forming bacterium that is widely distributed in nature. Its intrinsic thermal resistance coupled with the extraordinary resistance against common food preservation techniques makes it one of the most frequent food-poisoning microorganisms causing both intoxications and infections. In order to control B. cereus growth/sporulation, and hence minimize the aforementioned hazards, several antimicrobial compounds have been tested. The aim of this work was to assess by atomic force microscopy (AFM) the relationship between the molecular weight (MW) ofchitosan and its antimicrobial activity upon both vegetative and resistance forms of B. cereus. The use ofAFM imaging studies helped us to understand how chitosans with different MW act differently upon B. cereus. Higher MW chitosans (628 and 100 kDa) surrounded both forms of B. cereus cells by forming a polymer layer-which eventually led to the death of the vegetative form by preventing the uptake of nutrients yet did not affect the spores since these can survive for extended periods without nutrients. Chitooligosaccharides (COS) (<3kDa), on the other hand, provoked more visible damages in the B. cereus vegetative form-most probably due to the penetration of the cells by the COS. The use of COS by itself on B. cereus spores was not enough for the destruction ofa large number of cells, but it may well weaken the spore structure and its ability to contaminate, by inducing exosporium loss.
URI: http://hdl.handle.net/10400.14/2695
Versão do Editor: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TW1-4VW557X-3&_user=2460358&_coverDate=07%2F31%2F2009&_rdoc=8&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info%28%23toc%235549%232009%23998909991%231234075%23FLA%23display%23Volume%29&_cdi=5549&_sort=d&_docanchor=&_ct=48&_acct=C000057417&_version=1&_urlVersion=0&_userid=2460358&md5=2fe6ee24219ae7780f6cd7f7381b22c3&searchtype=a
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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