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Study of the antibacterial effects of chitosans on bacillus cereus (and its spores) by atomic force microscopy imaging and nanoindentation

2009Journal article Restricted access
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dc.contributor.authorFernandes, João C.
dc.contributor.authorEaton, Peter
dc.contributor.authorGomes, Ana M.
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2010-10-07T14:58:43Z
dc.date.available2010-10-07T14:58:43Z
dc.date.issued2009
dc.description.abstractBacillus cereus is a Gram-positive, spore-forming bacterium that is widely distributed in nature. Its intrinsic thermal resistance coupled with the extraordinary resistance against common food preservation techniques makes it one of the most frequent food-poisoning microorganisms causing both intoxications and infections. In order to control B. cereus growth/sporulation, and hence minimize the aforementioned hazards, several antimicrobial compounds have been tested. The aim of this work was to assess by atomic force microscopy (AFM) the relationship between the molecular weight (MW) of chitosan and its antimicrobial activity upon both vegetative and resistance forms of B. cereus. The use of AFM imaging studies helped us to understand how chitosans with different MW act differently upon B. cereus. Higher MW chitosans (628 and 100 kDa) surrounded both forms of B. cereus cells by forming a polymer layer—which eventually led to the death of the vegetative form by preventing the uptake of nutrients yet did not affect the spores since these can survive for extended periods without nutrients. Chitooligosaccharides (COS) (o3 kDa), on the other hand, provoked more visible damages in the B. cereus vegetative form—most probably due to the penetration of the cells by the COS. The use of COS by itself on B. cereus spores was not enough for the destruction of a large number of cells, but it may well weaken the spore structure and its ability to contaminate, by inducing exosporium loss.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citation"Ultramicroscopy". ISSN 0304-3991. 109: 8 (2009) 854–860por
dc.identifier.doi10.1016/j.ultramic.2009.03.015
dc.identifier.urihttp://hdl.handle.net/10400.14/2695
dc.language.isoengpor
dc.publisherElsevierpor
dc.subjectAtomicforcemicroscopy(AFM)por
dc.subjectChitosanpor
dc.subjectAntimicrobialpor
dc.subjectChitooligosaccharidepor
dc.titleStudy of the antibacterial effects of chitosans on bacillus cereus (and its spores) by atomic force microscopy imaging and nanoindentationpor
dc.typejournal article
dspace.entity.typePublication
person.familyNameFernandes
person.familyNameGomes
person.familyNamePintado
person.familyNameMalcata
person.givenNameJoão
person.givenNameAna Maria
person.givenNameMaria Manuela
person.givenNameFrancisco
person.identifier162628
person.identifier456608
person.identifier.ciencia-id881D-7FFD-DD7D
person.identifier.ciencia-idAA12-BFC1-B2E3
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-1556-1698
person.identifier.orcid0000-0001-7883-2446
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridK-3126-2013
person.identifier.ridB-9944-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id35274726800
person.identifier.scopus-author-id57190668768
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id7102542478
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscoveryc18c2fb1-f3dc-4274-a646-23d456432798

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