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COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection

2022-09-22Journal article Open access
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dc.contributor.authorEsteves, Eduardo
dc.contributor.authorMendes, Vera M.
dc.contributor.authorManadas, Bruno
dc.contributor.authorLopes, Rafaela
dc.contributor.authorBernardino, Liliana
dc.contributor.authorCorreia, Maria José
dc.contributor.authorBarros, Marlene
dc.contributor.authorEsteves, Ana Cristina
dc.contributor.authorRosa, Nuno
dc.date.accessioned2022-09-28T10:15:56Z
dc.date.available2022-09-28T10:15:56Z
dc.date.issued2022-09-22
dc.description.abstractCOVID-19 is the most impacting global pandemic of all time, with over 600 million infected and 6.5 million deaths worldwide, in addition to an unprecedented economic impact. Despite the many advances in scientific knowledge about the disease, much remains to be clarified about the molecular alterations induced by SARS-CoV-2 infection. In this work, we present a hybrid proteomics and in silico interactomics strategy to establish a COVID-19 salivary protein profile. Data are available via ProteomeXchange with identifier PXD036571. The differential proteome was narrowed down by the Partial Least-Squares Discriminant Analysis and enrichment analysis was performed with FunRich. In parallel, OralInt was used to determine interspecies Protein-Protein Interactions between humans and SARS-CoV-2. Five dysregulated biological processes were identified in the COVID-19 proteome profile: Apoptosis, Energy Pathways, Immune Response, Protein Metabolism and Transport. We identified 10 proteins (KLK 11, IMPA2, ANXA7, PLP2, IGLV2-11, IGHV3-43D, IGKV2-24, TMEM165, VSIG10 and PHB2) that had never been associated with SARS-CoV-2 infection, representing new evidence of the impact of COVID-19. Interactomics analysis showed viral influence on the host immune response, mainly through interaction with the degranulation of neutrophils. The virus alters the host’s energy metabolism and interferes with apoptosis mechanisms.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/jcm11195571pt_PT
dc.identifier.eid85139767427
dc.identifier.issn2077-0383
dc.identifier.pmcPMC9570692
dc.identifier.pmid36233441
dc.identifier.urihttp://hdl.handle.net/10400.14/39023
dc.identifier.wos000868033900001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCOVID-19pt_PT
dc.subjectSalivapt_PT
dc.subjectProteomicspt_PT
dc.subjectInteractomicspt_PT
dc.subjectOralintpt_PT
dc.titleCOVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue19pt_PT
oaire.citation.titleJournal of Clinical Medicinept_PT
oaire.citation.volume11pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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