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Extraction and characterization of extracellular polymeric substances from aerobic granular sludge from a full-scale sequencing batch reactor in Portugal

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dc.contributor.authorOliveira, Ana
dc.contributor.authorMesquita, Daniela P.
dc.contributor.authorAmorim, Catarina L.
dc.contributor.authorAmaral, A. L.
dc.contributor.authorFerreira, Eugénio C.
dc.contributor.authorLoosdrecht, Mark V.
dc.contributor.authorCastro, Paula M. L.
dc.date.accessioned2018-05-15T14:29:17Z
dc.date.available2018-05-15T14:29:17Z
dc.date.issued2017
dc.description.abstractAerobic granular sludge (AGS) is a recently developed technology for wastewater treatment. This system is able to manage higher amounts of wastewater and requires less surface area than conventional systems. The granules consist of microorganisms embedded in a self-produced extracellular polymeric substances (EPS) matrix. EPS are high molecular weight polymers, which can be metabolic products of microorganisms (e.g. proteins, polysaccharides, humic substances, nucleic acids) or be due to cell lyses. Accumulation on the cells surface of such EPS forms a protective barrier for the cells from the external environment. This work focus on the extraction and quantification of EPS from AGS from a large scale bioreactor in Portugal, during approximately 4 months. Given the environmental and chemical differences that these granules are subjected to one of the goals was to assess variability in the EPS production and characterize the granules morphology and composition in a large scale environment. EPS was extracted from AGS as described by Felz et al. using the sodium carbonate method. EPS biochemical characterization was made using colorimetric methods to access the proteins, polysaccharides and humic acids, and a fluorometric method to access the DNA content using a Qubit fluorometer. Quantitative image analysis was used to evaluate the size and several morphological parameters (e.g. roundness, robustness, compactness, and others) of the granules. Variations in the EPS composition were observed throughout the experience. Such variations can be due to chemical differences in the influent water and the fact that the loads can vary throughout the year. Consequently, the EPS production and composition in the granules was affected. Morphological parameters, namely roundness, compactness and robustness of the biomass are indicative factors of a stable granular biomass during the monitoring period. In summary, despite the fact that EPS composition changed in response to external stimuli, the granules showed morphological stability throughout the analysis.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.citationOliveira, A., Mesquita, D. P., Amorim, C. L., Amaral, A. L., Ferreira, E. C., Loosdrecht, M. V., Castro, P. M. L. (2018). Extraction and characterization of extracellular polymeric substances from aerobic granular sludge from a full-scale sequencing batch reactor in Portugal. MICROBIOTEC ´17 - 2017, Book of Abstracts - Congress of Microbiology and Biotechnology, 7-9 December, Porto, Portugal, p.45pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.14/24310
dc.language.isoengpt_PT
dc.relationNORTE-08-5369-FSE-000007pt_PT
dc.relationNORTE-01-0145-FEDER-000004pt_PT
dc.relationPOCI-01-0145-FEDER-006684pt_PT
dc.titleExtraction and characterization of extracellular polymeric substances from aerobic granular sludge from a full-scale sequencing batch reactor in Portugalpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardNumberUID/Multi/50016/2013
oaire.awardNumberSFRH/BPD/96481/2013
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F96481%2F2013/PT
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.titleMICROBIOTEC ´17 - Congress of Microbiology and Biotechnologypt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameOliveira
person.familyNameCastro
person.givenNameAna
person.givenNamePaula
person.identifier1415634
person.identifier2013444
person.identifier.ciencia-id4713-31C8-A356
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0001-8012-4203
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridJ-3404-2013
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id55937245800
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationbc42fa97-aa34-428f-b73f-d4327706efec
relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication.latestForDiscoveryf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isProjectOfPublication3fe5970e-de39-42f7-a29d-18521b591a09
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