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Advisor(s)
Abstract(s)
Antibiotics are a therapeutic class widely found inenvironmental matrices and
extensively studied due to its persistence and implications for multi-resistant bacteria
development. Degradation of four fluoroquinolone antibiotics, namely Ofloxacin (OFL),
Norfloxacin (NOR), Ciprofloxacin (CPF) and Moxifloxacin (MOX), at 10 mg L-1 using a
mixed bacterial culture, was assessed for 60 days. The assays were followed by a
developed and validated analytical method of HPLC with Fluorescence Detection using a
Luna PFP (2) 3µm column. The optimized conditions allowed picturing
metabolites/transformation products formation and accumulation during the process,
stating an incomplete mineralization, also shown byfluoride release. OFL and MOX
presented the highest (98.3%) and the lowest (80.5%) extent of degradation after 19 days
of assay, respectively. Some of these intermediate compounds were identified by LCMS/MS in selected degradation samples. Most of the intermediates were already
described as biodegradation and/or photodegradationproducts in different conditions,
but new and/or unknown metabolites were also present.
Description
Keywords
Biodegradation Fluoroquinolones LC-MS/MS Metabolites
Pedagogical Context
Citation
MAIA, Alexandra S. [et al.] - Enantioselective Determination of Fluoxetine and Norfluoxetine in Wastewater. In 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques, Amsterdam, The Netherlands, 16-20 June 2013 – In HPLC 2013 - 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques: Book of Abstracts, (2013) p.379