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Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages

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This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster analysis, it was possible to select the 2/2, N5, 2a, 135KP and 120 phages, as potential elements of a cocktail as a means of efficiently eliminating the greatest number of several types of Salmonella. The 2/2 and N5 phages were also the most efficacious infective elements against the Typhimurium and Enteritidis serovars, respectively.

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Salmonella spp. Bacteriophages Spot test Lytic spectra Phage typing Cluster analysis Discriminant analysis

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Citação

AMORIM, Lara R. P... [et al.] - Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteriophages. Food Control. ISSN 0956-7135. Vol. 21, n.º 5 (2010), p. 754-759

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