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Advisor(s)
Abstract(s)
The use of nanotechnology in medical sciences is an innovation that promises a new age of health. Among the different approaches explored so far, chitosan exhibits favourable and unique biological properties, such as biocompatibility, biodegradability, nonantigenic, non-toxicity and mucoadhesiveness. Natural extracts rich in antioxidant molecules have been incorporated in chitosan films, or macro/microparticles, becoming more effective as improving the antioxidant protection [da Silva 2010]. In the present study, chitosan nanoparticles with extracts of sage and savoury and rosmarinic acid were prepared and characterized in order to ensure their best size, efficient encapsulation and to test the retention of the active compounds and evaluate their controlled release performance. This work proposes for the first time in literature, a simultaneous HPLC method for the determination and quantification of phenolic and flavonoid compounds, namely quercetin and rosmarinic acid encapsulated into chitosan nanoparticles. The method can be used to determine the loading capacity and association efficiency as well as its in vitro release.