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Clonal interference and genomic repair during strain coexistence in the gut

2025-07-07Artigo de investigação Acesso aberto
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dc.contributor.authorFrazão, Nelson
dc.contributor.authorSeixas, Elsa
dc.contributor.authorMischler, Manolo
dc.contributor.authorMoura-de-Sousa, Jorge
dc.contributor.authorBarreto, Hugo C.
dc.contributor.authorGordo, Isabel
dc.date.accessioned2025-09-02T13:34:12Z
dc.date.available2025-09-02T13:34:12Z
dc.date.issued2025-07-07
dc.description.abstractHumans and other mammals are colonized by multiple strains of Escherichia coli, but the tempo and mode of evolution of different coexisting strains, between whom horizontal gene transfer (HGT) can occur, is poorly understood. Here, we follow in real time the evolution of two phylogenetic distinct strains of E. coli that co-colonize the mouse gut with different population sizes. We find qualitative differences in evolutionary dynamics between strains within the same host. In the strain with larger population size intense clonal interference occurs and polymorphism at a neutral marker locus is maintained, while in the strain with lower population size complete selective sweeps and loss of neutral marker polymorphism occurs. Strain coexistence is also accompanied by rich dynamics of HGT from one strain to the other. Strikingly, a rare HGT event could restore a previously lost genomic region in the recipient strain. Furthermore, we detect for the first time a case of phage piracy in the gut, where a putative phage satellite, lacking essential genes for their own replication, was likely mobilized by a helper phage to transfer between bacterial hosts. Our results show that HGT is a key mechanism underlying genetic exchanges and adaptive genomic repair in the mammalian gut.eng
dc.identifier.citationFrazão, N., Seixas, E., Mischler, M., & Moura-de-Sousa, J. et al. (2025). Clonal interference and genomic repair during strain coexistence in the gut. PLoS Genetics, 21(7), Article e1011777. https://doi.org/10.1371/journal.pgen.1011777
dc.identifier.doi10.1371/journal.pgen.1011777
dc.identifier.eid105009910087
dc.identifier.issn1553-7390
dc.identifier.otherf5a80db0-897f-4409-afae-4e7a550671f3
dc.identifier.pmcPMC12251178
dc.identifier.pmid40623055
dc.identifier.urihttp://hdl.handle.net/10400.14/54605
dc.identifier.wos001524169900004
dc.language.isoeng
dc.peerreviewedyes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleClonal interference and genomic repair during strain coexistence in the guteng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.issue7
oaire.citation.titlePLoS Genetics
oaire.citation.volume21
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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